Art. -Nr.: HY-D1533

RhoNox-1

45 Cited Publications

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Based on 45 publication(s) in Google Scholar

RhoNox-1 is a fluorescent probe for the specific detection of divalent iron ions, and when RhoNox-1 reacts with Fe²⁺. RhoNox-1 can generate an irreversible orange (red) fluorescent product (Ex/Em：540/575 nm) . FeRhoNox-1 can enter the cell well, suitable for the detection of Fe²⁺ in living cells, and tends to be localized in the Golgi apparatus.

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Reinheit: 98.54%
CAS. Nr.: 1447815-38-4
Formel: C₂₈H₃₀N₂O₄
Molecular Weight:458.55

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

RhoNox-1 Chemical Structure

CAS. Nr.: 1447815-38-4

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
- Emission (Em)
- Excitation (Ex)
Chemical Information
Publications (45)
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of RhoNox-1 working solution
1.1 Preparation of the stock solution
Dissolve RhoNox-1 in DMSO to obtain 1 mM of stock solution.
1.2 Preparation of RhoNox-1 working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of RhoNox-1 working solution according to the actual situation.
2. Cell staining (Suspension cells)
2.1 Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×10⁶/mL.
2.2 Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
3. Cell staining (Adherent cells)
3.1 Culture adherent cells on sterile coverslips.
3.2 Remove the coverslip from the medium and aspirate excess medium.
3.3 Add 100 μL of working solution, gently shake it to completely cover the cells, and then incubate at room temperature for 5-30 minutes.
3.4 Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Emission (Em)

575

Excitation (Ex)

540

Chemical Information

CAS. Nr. 1447815-38-4
Appearance Solid
Molecular Weight 458.55
Formel C₂₈H₃₀N₂O₄
Color Pink to red
SMILES

O=C(C1=C(C2=C3C=C/C(C=C3OC4=CC([N+](CC)(CC)[O-])=CC=C42)=[N+](CC)\CC)C=CC=C1)[O-]
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (45)

All Flow CytometryCell Imaging/Staining

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Journal Impact Factor

Nat Commun

Iron overload in the tumor microenvironment induces CD8+ T cell ferroptosis and dysfunction. [Abstract]

2026 May 22. PMID: 42173913
J Nanobiotechnology

Therapeutic translation of traditional Chinese medicine Huangqi derived exosome like nanoparticles: targeting prostate cancer through ferroptosis activation, immune reprogramming, and microbiome modulation. [Abstract]

2026 Mar 11. PMID: 41814394
J Nanobiotechnology

Anthocyanin-functionalized selenocysteine nanotherapeutics alleviate cisplatin nephrotoxicity by inhibiting oxidative stress and ferroptosis. [Abstract]

2025 Nov 5;23(1):703. PMID: 41194109

RhoNox-1 purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Nov 5;23(1):703. [Abstract]

RhoNox‐1 (1 µM; 30 min). Representative images of HK-2 cells stained with RhoNox-1 probe.
J Nanobiotechnology

Quadruple synergistic amplification of ferroptosis for precision glioblastoma therapy: a luteolin-coordinated ferric ion nanoplatform. [Abstract]

2025 Sep 25;23(1):605. PMID: 40999469
Adv Sci (Weinh)

Piezo1 Upregulation in Monocyte-Derived Macrophages Impairs Post-Myocardial Infarction Cardiac Repair via Defective Efferocytosis and Enhanced Ferroptosis. [Abstract]

2025 Nov 10:e10991. PMID: 41214880

RhoNox-1 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 10:e10991. [Abstract]

RhoNox‐1 (10 µM; 30 min). The levels of Fe2+ in BMDMs were analyzed by immunostaining and flow cytometry analysis of FeRhoNox-1.
Adv Sci (Weinh)

PHOSPHO1 Suppresses Ferroptosis in Retinal Pigment Epithelial Cells by Reducing the Levels of Phosphatidylethanolamine Molecular Species. [Abstract]

2025 Jul;12(28):e2505359. PMID: 40396905

RhoNox-1 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul;12(28):e2505359. [Abstract]

Intracellular Fe²⁺ levels under 30 µM Erastin or 500 µM FAC (Ammonium iron(III) citrate) treatment for 4 h were detected with the RhoNox-1 (1μM; 1 h) fluorescent probe.
Biomaterials

Self-amplifying nanomedicine reprograms redox metabolism to trigger immunogenic ferroptosis in colon cancer: multiomics identifies AMPD3 as a novel regulator. [Abstract]

2026 Oct:333:124196. PMID: 41950646
Research (Wash D C)

Targeting the Exonic Circular OGT RNA/O-GlcNAc Transferase/Forkhead Box C1 Axis Inhibits Asparagine- and Alanine-Mediated Ferroptosis Repression in Neuroblastoma Progression. [Abstract]

2025 May 23:8:0703. PMID: 40416363
J Control Release

Polyhedral magnetic nanoparticles induce apoptosis in gastric cancer stem cells and suppressing tumor growth through magnetic force generation. [Abstract]

2024 Jul 24:373:370-384. PMID: 39032573
Cell Death Dis

Selenomethionine as a dual-mechanism ferroptosis inhibitor: selenium-supply-driven GPX4 biosynthesis beyond transsulfuration and reductive-capacity-mediated ROS scavenging independent of GPX4 activity. [Abstract]

2026 Feb 14;17(1):224. PMID: 41688429
Cell Commun Signal

Uncovering the role of ferroptosis in Bietti crystalline dystrophy and potential therapeutic strategies. [Abstract]

2024 Jul 11;22(1):359. PMID: 38992691
Phytomedicine

Synergistic effects of heterophyllin B with nintedanib against experimental pulmonary fibrosis in mice. [Abstract]

2025 Aug:144:156922. PMID: 40513322
Adv Healthc Mater

Membrane Intercalation of a Conjugated Oligoelectrolyte Photosensitizer Enables Efficient Anticancer Photodynamic Therapy. [Abstract]

2025 Jul;14(18):e2501300. PMID: 40411856
Apoptosis

Adverse outcome pathway analysis identifies DRP1-driven mitochondrial dysfunction as a central event in silver nanoparticle-induced hepatocyte ferroptosis. [Abstract]

2026 Mar 19;31(3):103. PMID: 41851563
Int J Biol Macromol

The STING-OLA1-Keap1-Nrf2 axis regulates ferroptosis in premature ovarian failure. [Abstract]

2025 Dec 4;337(Pt 1):149424. PMID: 41352507
Microbiol Res

Copper gluconate drives adherent-invasive Escherichia coli LF82 into a viable-but-non-culturable state: Mechanisms of persistence and susceptibility. [Abstract]

2026 May:306:128457. PMID: 41619420
Free Radic Biol Med

Tartrate-Resistant Acid Phosphatase 5 (TRAP/ACP5) aggravates atherosclerosis by regulating macrophage polarization and promoting ferroptosis. [Abstract]

2026 Apr:247:486-501. PMID: 41692316
Free Radic Biol Med

Brazilin and its oxidized form "brazilein", the natural fluorescently traceable agents, toxify Plasmodium falciparum to pyknotic death via disrupting its iron homeostasis. [Abstract]

2026 Feb 28:248:367-385. PMID: 41771360
Free Radic Biol Med

Cigarette smoke disrupts osteogenic-adipogenic balance via Nrf2/HERC2 axis-driven ferroptosis. [Abstract]

2025 Sep 10:S0891-5849(25)00971-2. PMID: 40939850
ACS Appl Mater Interfaces

Nanovesicles Synergistically Regulate Ferroptosis Characteristic Macrophage-Induced Angiogenesis via Ferritin Heavy Chain 1/Glutathione Peroxidase 4 Pathway to Promote Peripheral Nerve Regeneration. [Abstract]

2025 Oct 8;17(40):55876-55894. PMID: 40958080
ACS Appl Mater Interfaces

Polydopamine@Iron/Ellagic Acid Nanoparticles: Self-Reinforcing Ferroptosis-Apoptosis Synergy Disrupts Energy Metabolism for Melanoma Therapy. [Abstract]

2025 Oct 22;17(42):57903-57918. PMID: 41074794

RhoNox-1 purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2025 Oct 22;17(42):57903-57918. [Abstract]

RhoNox-1 (10 μM; 1 h). Fluorescence images showing intracellular Fe²⁺ (red; FeRhoNox-1) and nuclear staining (blue; Hoechst) in B16 cells after incubation with PFE-PEG NPs or PFE-PEG NPs plus NIR irradiation.
J Med Chem

Discovery of a Hydroxamic Acid-Based HDAC11 Isoform-Selective Inhibitor with Oral Anti-AML Potency. [Abstract]

2026 Mar 26;69(6):6816-6834. PMID: 41787751
J Med Chem

HDAC11 Inhibition as a Potential Therapeutic Strategy for AML: Target Identification, Lead Discovery, Antitumor Potency, and Mechanism Investigation. [Abstract]

2025 Apr 24;68(8):8124-8142. PMID: 40177883

RhoNox-1 purchased from MedChemExpress. Usage Cited in: J Med Chem. 2025 Apr 24;68(8):8124-8142. [Abstract]

RhoNox-1 (5 μM; 20 min). Flow cytometry analysis for intracellular Fe²⁺ levels (RhoNox-1) in U937 cell treated with DMSO or A9 for 48 h.
J Ethnopharmacol

Sanwu Huangqin decoction induces ferroptosis in colorectal cancer cells by triggering NCOA4/FTH1-mediated ferritinophagy. [Abstract]

2026 Oct 28:369:121932. PMID: 42219071
Ecotoxicol Environ Saf

Blue LED light induces dry eye disease via NCOA4-mediated ferritinophagy and iron-overload. [Abstract]

2026 May:316:120150. PMID: 42019352
Ecotoxicol Environ Saf

Integrating network pharmacology and experimental validation to decipher the protective effect of icariin against testicular aging. [Abstract]

2025 Sep 15:303:119063. PMID: 40961599
Biochem Pharmacol

PTBP1 acts as a tumor suppressor in glioma by promoting HMOX1-dependent ferroptosis. [Abstract]

2025 Sep:239:117041. PMID: 40484043
Life Sci

M2 macrophages secrete CCL20 to regulate iron metabolism and promote daunorubicin resistance in AML cells. [Abstract]

2025 Jan 15:361:123297. PMID: 39645162
Commun Biol

Resveratrol offers therapeutic potential in obesity-related osteoarthritis by targeting ferroptosis via G3BP1-SGs/mTOR modulation. [Abstract]

2026 Jun 3. PMID: 42237016
Commun Biol

Zingerone treats postmenopausal osteoporosis via increased ferroptosis sensitivity by p53-mediated regulation of SAT1 and GPX4 expression. [Abstract]

2025 Sep 26;8(1):1367. PMID: 41006700
Int J Mol Sci

Investigating the Role of Peroxisomes in Regulating Breast Cancer Stem Cell Mechanisms. [Abstract]

2025 Nov 25;26(23):11389. PMID: 41373547
Int Immunopharmacol

Oltipraz attenuated cerebral ischemia-reperfusion injury through inhibiting the oxidative stress and ferroptosis in mice. [Abstract]

2024 Oct 25:140:112800. PMID: 39096875
Mol Neurobiol

ROS Regulate Rotenone-induced SH-SY5Y Dopamine Neuron Death Through Ferroptosis-mediated Autophagy and Apoptosis. [Abstract]

2025 Jul;62(7):9271-9289. PMID: 40097764
Chem Res Toxicol

Nrf2 Activation Mitigates Silver Nanoparticle-Induced Ferroptosis in Hepatocytes. [Abstract]

2025 Sep 15;38(9):1485-1494. PMID: 40888047
J Biol Chem

Exosomes drive ferroptosis by stimulating iron accumulation to inhibit bacterial infection in crustaceans. [Abstract]

2023 Dec;299(12):105463. PMID: 37977221
Naunyn Schmiedebergs Arch Pharmacol

Andrographolide blocked the progression of endometriosis by promoting ferroptosis via inhibiting anabolism of serine. [Abstract]

2025 Dec 18. PMID: 41408483
J Photochem Photobiol B

Blue light induced ferroptosis via STAT3/GPX4/SLC7A11/FTH1 in conjunctiva epithelium in vivo and in vitro. [Abstract]

2024 Jun:255:112908. PMID: 38663336
PLoS Genet

Age-associated decline of Coenzyme A leads to intestinal stem cells dysfunction via disturbing iron homeostasis. [Abstract]

2025 May 30;21(5):e1011704. PMID: 40446021
J Funct Foods

2025 Jun.
Neurotoxicology

Insights into cytotoxicity and redox modulation by the herbicide linuron and its metabolite, 3,4-dichloroaniline. [Abstract]

2025 Sep 17:103324. PMID: 40972898
Int J Biochem Cell Biol

Chromobox protein homolog 7 (CBX7) deficiency inhibits osteoblast ferroptosis by activating the Nrf2 function in type 2 diabetic osteoporosis. [Abstract]

2025 Dec:189:106866. PMID: 40998236
Theriogenology

Regulation of autophagy-Ferroptosis crosstalk by the SLC7A11-GPX4 axis during sperm capacitation. [Abstract]

2026 Apr 1:254:117795. PMID: 41483535
Indian J Ophthalmol

Ranitidine protects Müller cells against ferroptosis in diabetic retinopathy by regulating the AKT1/GSK3β pathway. [Abstract]

2026 Feb 1;74(2):250-258. PMID: 41581041
J Clin Pharm Ther

2026.
SSRN

2025 Jan 14.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (218.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1808 mL

10.9039 mL

21.8079 mL

5 mM

0.4362 mL

2.1808 mL

4.3616 mL

10 mM

0.2181 mL

1.0904 mL

2.1808 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Mukaide T, et al. Histological detection of catalytic ferrous iron with the selective turn-on fluorescent probe RhoNox-1 in a Fenton reaction-based rat renal carcinogenesis model. Free Radic Res. 2014 Sep;48(9):990-5. [Content Brief]

[2]. Jamnongkan W, et al. Upregulation of transferrin receptor-1 induces cholangiocarcinoma progression via induction of labile iron pool. Tumour Biol. 2017 Jul;39(7):1010428317717655. [Content Brief]

[3]. Ito F, et al. Contrasting intra- and extracellular distribution of catalytic ferrous iron in ovalbumin-induced peritonitis. Biochem Biophys Res Commun. 2016 Aug 5;476(4):600-606. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1808 mL	10.9039 mL	21.8079 mL	54.5197 mL
	5 mM	0.4362 mL	2.1808 mL	4.3616 mL	10.9039 mL
	10 mM	0.2181 mL	1.0904 mL	2.1808 mL	5.4520 mL
	15 mM	0.1454 mL	0.7269 mL	1.4539 mL	3.6346 mL
	20 mM	0.1090 mL	0.5452 mL	1.0904 mL	2.7260 mL
	25 mM	0.0872 mL	0.4362 mL	0.8723 mL	2.1808 mL
	30 mM	0.0727 mL	0.3635 mL	0.7269 mL	1.8173 mL
	40 mM	0.0545 mL	0.2726 mL	0.5452 mL	1.3630 mL
	50 mM	0.0436 mL	0.2181 mL	0.4362 mL	1.0904 mL
	60 mM	0.0363 mL	0.1817 mL	0.3635 mL	0.9087 mL
	80 mM	0.0273 mL	0.1363 mL	0.2726 mL	0.6815 mL
	100 mM	0.0218 mL	0.1090 mL	0.2181 mL	0.5452 mL

Keywords

RhoNox-1

45 Cited Publications

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RhoNox-1

Publications Citing Use of MedChemExpress (MCE) RhoNox-1

Biologische Aktivität

Chemical Information

Publications (45)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

RhoNox-1 Related Classifications

HY-D1533 Related Classifications