SBI-425
Based on 5 publication(s) in Google Scholar
SBI-425 is an orally active and potent TNAP (tissue-nonspecific alkaline phosphatase) inhibitor (IC50=16 nM). SBI-425 inhibits TNAP in the vasculature, improving cardiovascular parameters and survival.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 98.88%
- CAS. Nr.: 1451272-71-1
- Formel: C13H12ClN3O4S
- Molecular Weight:341.77
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Metabolism/Protease Compound Library
HY-L012
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Anti-Cardiovascular Disease Compound Library
HY-L046
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Orally Active Compound Library
HY-L061
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Phosphatase Inhibitor Library
HY-L081
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Heterocyclic Compound Library
HY-L138
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Metabolic Enzyme Compound Library
HY-L146
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Highly Selective Inhibitors Library
HY-L158
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
Publications Citing Use of MedChemExpress (MCE) SBI-425
More
Biologische Aktivität
IC50: 16 nM (TNAP)[1]
| Compds | SBI-425 |
| Clp (mL/min/kg) | 5.14 |
| Vd (L/kg) | 1.03 |
| Cmax (μg/mL) | 178 |
| AUC (μg*hr/mL) | 848 |
| t1/2 (hr) | 2.3 |
| %F | 58 |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 1451272-71-1
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Appearance Solid
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Molecular Weight 341.77
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Formel C13H12ClN3O4S
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Color White to off-white
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SMILES
O=C(C1=CC(NS(=O)(C2=CC(Cl)=CC=C2OC)=O)=CN=C1)N
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (5)
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Journal Impact Factor
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Most Recent
Lösungsmittel & Löslichkeit
DMSO : 86.67 mg/mL (253.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.17 mg/mL (6.35 mM); Clear solution
This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Pinkerton AB, et al. Discovery of 5-((5-chloro-2-methoxyphenyl)sulfonamido)nicotinamide (SBI-425), a potent and orally bioavailable tissue-nonspecific alkaline phosphatase (TNAP) inhibitor. Bioorg Med Chem Lett. 2018 Jan 1;28(1):31-34. [Content Brief]
[2]. Sheen CR, et al. Pathophysiological role of vascular smooth muscle alkaline phosphatase in medial artery calcification. J Bone Miner Res. 2015 May;30(5):824-36. [Content Brief]
[3]. Brichacek AL, et al. Systemic inhibition of tissue-nonspecific alkaline phosphatase alters the brain-immune axis in experimental sepsis. Sci Rep. 2019 Dec 11;9(1):18788. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9259 mL | 14.6297 mL | 29.2594 mL | 73.1486 mL |
| 5 mM | 0.5852 mL | 2.9259 mL | 5.8519 mL | 14.6297 mL | |
| 10 mM | 0.2926 mL | 1.4630 mL | 2.9259 mL | 7.3149 mL | |
| 15 mM | 0.1951 mL | 0.9753 mL | 1.9506 mL | 4.8766 mL | |
| 20 mM | 0.1463 mL | 0.7315 mL | 1.4630 mL | 3.6574 mL | |
| 25 mM | 0.1170 mL | 0.5852 mL | 1.1704 mL | 2.9259 mL | |
| 30 mM | 0.0975 mL | 0.4877 mL | 0.9753 mL | 2.4383 mL | |
| 40 mM | 0.0731 mL | 0.3657 mL | 0.7315 mL | 1.8287 mL | |
| 50 mM | 0.0585 mL | 0.2926 mL | 0.5852 mL | 1.4630 mL | |
| 60 mM | 0.0488 mL | 0.2438 mL | 0.4877 mL | 1.2191 mL | |
| 80 mM | 0.0366 mL | 0.1829 mL | 0.3657 mL | 0.9144 mL | |
| 100 mM | 0.0293 mL | 0.1463 mL | 0.2926 mL | 0.7315 mL |
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