Calcein-AM

Calcein-AM purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2022 Aug 11;21:20-31. [Abstract]

Customer Validation
HUVECs proliferation is assessed by Calcein-AM, and the results show that HP-β-CD and HP-β-CD@Res could effectively promote the proliferation of HUVECs.

Calcein-AM purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 Oct;9(30):e2203031. [Abstract]

Customer Validation
XPS analysis of CaALN powder before and after immersing in FeCl3 solution for 24 h. Detection of intracellular Fe concentration using Calcein-AM in MGC 803 cells after treatment with NaALN, CaALN, and DFX at 25 mg/L for 24 h by FCM analysis. Then these cells are washed with PBS and incubated with Calcein-AM (0.5 μM) for 15 min at 37 °C in the dark.

Calcein-AM purchased from MedChemExpress. Usage Cited in: Pharmaceuticals. 2022 Aug 26;15(9):1059. [Abstract]

Customer Validation
For U251 cells, Calcein-AM (10 μM) solution and 0.5 μL PI staining solution (15 μM) were added into each well and stained for 10 min.

Calcein-AM purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2021 May 18;10(5):798. [Abstract]

Customer Validation
HeLa cells were pretreated with 20 μM Fe(II) for 2 h and then incubated with Calcein-AM (0.5 μM). After 15 min, cells were washed and incubated with or without CAPE and its analogues for 15 min. The fluorescence intensity of cells was measured by flow cytometry.

Calcein-AM purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2021 Jun:188:114583. [Abstract]

Customer Validation
Cells were incubated with fluorescence probes Calcein-AM (1 μM) and propidium iodide (5 μM) for 5 min and imaged through a fluorescence inverted microscope.

Calcein-AM purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2020 Sep;10(9):1694-1708. [Abstract]

Customer Validation
HUVECs were pre-treated with SMI or Rd for 24 h and seeded on Matrigel for 5.5 h followed by 0.5 h of Calcein-AM staining.

Calcein-AM purchased from MedChemExpress. Usage Cited in: EMBO Rep. 2019 Jul;20(7):e47563. [Abstract]

Customer Validation
Cells were trypsinized, washed twice with 0.5 ml PBS, and then incubated with 0.2 μM Calcein‐AM at 37°C for 15 min. Then, the cells were washed twice with 0.5 ml PBS and either incubated with 100 μM Deferiprone or left untreated at 37°C for 1 h. The cells were analyzed with a flow cytometer. Calcein-AM was excited at 488 nm, and fluorescence was measured at 525 nm.