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  2. Fluorescent Dye
  3. ATTO 550

ATTO 550 is a new type of fluorescent dye with high fluorescence yield, which can be used for most labeling applications, maximum excitation/emission wavelength: 554/576 nm.

For research use only. We do not sell to patients.

ATTO 550

ATTO 550 Chemical Structure

CAS No. : 1251752-59-6

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Description

ATTO 550 is a new type of fluorescent dye with high fluorescence yield, which can be used for most labeling applications, maximum excitation/emission wavelength: 554/576 nm.

Molecular Weight

694.21

Formula

C37H44ClN3O8

CAS No.
Appearance

Solid

Color

Brown to reddish brown

SMILES

O=C(CCCN(C)C(C1=C(C2=C3C=C4C(C)CC(C)(C)NC4=CC3=[O+]C5=C2C=C6C(NC(C)(CC6C)C)=C5)C=CC=C1)=O)O.O=Cl(=O)([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

Ethanol : 20 mg/mL (28.81 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4405 mL 7.2024 mL 14.4049 mL
5 mM 0.2881 mL 1.4405 mL 2.8810 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Concentration (final)

C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol 1 mM 1.4405 mL 7.2024 mL 14.4049 mL 36.0122 mL
5 mM 0.2881 mL 1.4405 mL 2.8810 mL 7.2024 mL
10 mM 0.1440 mL 0.7202 mL 1.4405 mL 3.6012 mL
15 mM 0.0960 mL 0.4802 mL 0.9603 mL 2.4008 mL
20 mM 0.0720 mL 0.3601 mL 0.7202 mL 1.8006 mL
25 mM 0.0576 mL 0.2881 mL 0.5762 mL 1.4405 mL
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
ATTO 550
Cat. No.:
HY-D2017
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