Cat. No.: HY-147092

Oryzalin

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

Oryzalin is a dinitroaniline herbicide with antimitotic and antileishmanial activities. Oryzalin can bind to plant tubulin, inhibit the polymerization of microtubules in plant cells, and affect chromosome migration. Oryzalin can also be used to induce chromosome doubling.

For research use only. We do not sell to patients.

Purity: 99.62%
CAS No.: 19044-88-3
Formula: C₁₂H₁₈N₄O₆S
Molecular Weight:346.36

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Oryzalin Chemical Structure

CAS No.: 19044-88-3

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Publications Citing Use of MedChemExpress (MCE) Oryzalin

Nat Commun. 2025 Jul 18;16(1):6621. [Abstract]

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Cell Imaging/Staining

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Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-8	IC₅₀	800 nM Compound: 2	Antimicrobial activity against Cryptosporidium parvum infected in HCT8 cells assessed as parasite count after 72 to 120 hrs Antimicrobial activity against Cryptosporidium parvum infected in HCT8 cells assessed as parasite count after 72 to 120 hrs	[PMID: 21889338]
HFF	IC₅₀	0.25 μM Compound: 10	Antimicrobial activity against Toxoplasma gondii RH infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	1.26 μM Compound: 10	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin F24H mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin F24H mutation infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	1.4 μM Compound: 10	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin H28Q mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin H28Q mutation infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	1.5 μM Compound: 10	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin S6I mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin S6I mutation infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	1.6 μM Compound: 10	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin I235L mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin I235L mutation infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	14.7 μM Compound: 10	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin V4L mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin V4L mutation infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	16 μM Compound: 10	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin R243S mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin R243S mutation infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	2.2 μM Compound: 10	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin R243C mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin R243C mutation infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	2.3 μM Compound: 10	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin I235V mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin I235V mutation infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	25.3 μM Compound: 10	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin T239I mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin T239I mutation infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	27.7 μM Compound: 10	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin L136F mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin L136F mutation infected in HFF cells by plaque assay	[PMID: 20145086]
HFF	IC₅₀	5.5 μM Compound: 10	Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin I235T mutation infected in HFF cells by plaque assay Antimicrobial activity against Toxoplasma gondii RH containing alpha1-tubulin I235T mutation infected in HFF cells by plaque assay	[PMID: 20145086]
J774	IC₅₀	44 μM Compound: 2 (Oryzalin)	In vitro cytotoxicity against mouse J774 macrophages. In vitro cytotoxicity against mouse J774 macrophages.	[PMID: 15027874]
PC-3	IC₅₀	56 μM Compound: 2 (Oryzalin)	In vitro cytotoxicity against mouse PC3 prostate cancer cell line. In vitro cytotoxicity against mouse PC3 prostate cancer cell line.	[PMID: 15027874]

In Vitro

Oryzalin (0.1 μM; 5 min) inhibits anaphase chromosome migration, depolymerizes microtubules, and prevents the polymerization of new microtubules at all stages of the mitotic cycle in the endosperm cells of Haemanthus katherinae Bak^[1].
Oryzalin (5-30 μM; in agar solution for 20-30 days) can double the chromosomes in isolated haploid apple stem segments with high survival rate and efficiency^[2].
Oryzalin (6-500 μM; 1 h) has high hemolytic activity in human red blood cells, with a hemolysis rate of approximately 20% at 100 μM^[2].
Oryzalin (6-50 μM; 48 h) has antileishmanial activity in THP-1 cells infected with L. infantum, with an IC₅₀ of 24.2 μM^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 19044-88-3
Appearance Solid
Molecular Weight 346.36
Formula C₁₂H₁₈N₄O₆S
Color Orange to red
SMILES

O=S(C1=CC([N+]([O-])=O)=C(N(CCC)CCC)C([N+]([O-])=O)=C1)(N)=O
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

All Cell Imaging/Staining

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Journal Impact Factor

Nat Commun

ATG8ylation-mediated tonoplast invagination mitigates vacuole damage. [Abstract]

2025 Jul 18;16(1):6621. PMID: 40681515

Oryzalin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 18;16(1):6621. [Abstract]

Oryzalin (10 μM) and latrunculin B (10 μM) were employed to disrupt microtubule and microfilament structures, respectively.

Solvent & Solubility

In Vitro:

DMSO : 233.33 mg/mL (673.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8872 mL

14.4358 mL

28.8717 mL

5 mM

0.5774 mL

2.8872 mL

5.7743 mL

10 mM

0.2887 mL

1.4436 mL

2.8872 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (623 KB)
- English - EN (623 KB)
- Français - FR (623 KB)
- Deutsch - DE (623 KB)
- Norwegian - NO (623 KB)
- Español - ES (623 KB)
- Swedish - SV (623 KB)
- Italian - IT (623 KB)
- Korean - KR (623 KB)
- Portuguese - PT (623 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Morejohn LC, et al. Oryzalin, a dinitroaniline herbicide, binds to plant tubulin and inhibits microtubule polymerization in vitro. Planta. 1987 Oct;172(2):252-64. [Content Brief]

[2]. Bouvier L, et al. Oryzalin as an efficient agent for chromosome doubling of haploid apple shoots in vitro. Plant Breeding, 1994, 113(4): 343-346.

[3]. Lopes RM, et al. Formulation of oryzalin (ORZ) liposomes: in vitro studies and in vivo fate. Eur J Pharm Biopharm. 2012 Oct;82(2):281-90. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.8872 mL	14.4358 mL	28.8717 mL	72.1792 mL
	5 mM	0.5774 mL	2.8872 mL	5.7743 mL	14.4358 mL
	10 mM	0.2887 mL	1.4436 mL	2.8872 mL	7.2179 mL
	15 mM	0.1925 mL	0.9624 mL	1.9248 mL	4.8119 mL
	20 mM	0.1444 mL	0.7218 mL	1.4436 mL	3.6090 mL
	25 mM	0.1155 mL	0.5774 mL	1.1549 mL	2.8872 mL
	30 mM	0.0962 mL	0.4812 mL	0.9624 mL	2.4060 mL
	40 mM	0.0722 mL	0.3609 mL	0.7218 mL	1.8045 mL
	50 mM	0.0577 mL	0.2887 mL	0.5774 mL	1.4436 mL
	60 mM	0.0481 mL	0.2406 mL	0.4812 mL	1.2030 mL
	80 mM	0.0361 mL	0.1804 mL	0.3609 mL	0.9022 mL
	100 mM	0.0289 mL	0.1444 mL	0.2887 mL	0.7218 mL

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Oryzalin

Anti-Infection Compound Library

Cell Cycle/DNA Damage Compound Library

Metabolism/Protease Compound Library

Anti-Aging Compound Library

Cytoskeleton Compound Library

Anti-Lung Cancer Compound Library

Protein-protein Interaction Inhibitor Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Pesticide Compound Library

High-Throughput Bioactive Compound Library

High-Efficiency Gene Editing Compound Library

Environmental Pollutant Compound Library

Publications Citing Use of MedChemExpress (MCE) Oryzalin

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Oryzalin

Anti-Infection Compound Library

Cell Cycle/DNA Damage Compound Library

Metabolism/Protease Compound Library

Anti-Aging Compound Library

Cytoskeleton Compound Library

Anti-Lung Cancer Compound Library

Protein-protein Interaction Inhibitor Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Pesticide Compound Library

High-Throughput Bioactive Compound Library

High-Efficiency Gene Editing Compound Library

Environmental Pollutant Compound Library

Publications Citing Use of MedChemExpress (MCE) Oryzalin

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Oryzalin Related Classifications

HY-147092 Related Classifications