AZD8701
Based on 1 Customer Validation
AZD8701 (IONIS-1063734) is an antisense oligonucleotide targeting FOXP3 in regulatory T cells (Tregs), with a human IC50 of 65.2 nM. AZD8701 binds to intronic sites of all FOXP3 pre-mRNA isoforms and mediates dose-dependent FOXP3 knockdown via free uptake. AZD8701 can be used in cancer-related research.
For research use only. We do not sell to patients.
- Purity: 96.61%
- CAS No.: 2603457-08-3
- Molecular Weight:5355.77
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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FOXP3 65.2 nM (IC50) |
AZD8701 (65 nM) potently knocks down FOXP3 in primary human Tregs (IC50 65 nM) via free uptake, reduces expression of key FOXP3 target genes by 25-50%, and impairs Treg suppressive function in vitro[1].
AZD8701 (0.001-10 μM; 9 days) potently reduces FOXP3 protein expression in primary human Tregs (EC50 = 44 nM, IC50 = 65.2 nM) and downregulates FOXP3-dependent immunosuppressive biomarkers CTLA4, ICOS, GITR, and CCR8 at 1 μM[2].
AZD8701 (1 μM; 9 days) strongly reduces FOXP3 protein expression in human thymic natural Tregs and effector Tregs subsets from healthy donor PBMCs[2].
AZD8701 reduces the suppressive function of human inducible Tregs in an in vitro effector T cell proliferation assay[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
AZD8701 (6.25-50 mg/kg; s.c.; daily; 4 days) drives dose-dependent knockdown of FOXP3 mRNA and protein in human Tregs in humanized NSG mice[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) (female, engrafted with 1×107 human peripheral blood mononuclear cells)[2]
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Dosage:6.25 mg/kg; 12.5 mg/kg; 25 mg/kg; 50 mg/kg
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Administration:s.c.; daily; 4 days
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Result:Promoted dose-dependent knockdown of FOXP3 mRNA in splenic human Tregs.
Promoted significant downregulation of FOXP3 protein levels in splenic, blood, and bone marrow human Tregs at 25 mg/kg, reducing the percentage of FOXP3+ CD4+ T cells in spleen.
Chemical Information
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CAS No. 2603457-08-3
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Appearance Solid
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Molecular Weight 5355.77
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Color White to off-white
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SMILES
[AZD8701]
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Synonyms
IONIS-1063734
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2242 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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