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BAMEA-O16B 

Cat. No.: HY-139306 Purity: ≥98.0%
Handling Instructions

BAMEAO16B is a lipid nanoparticle. BAMEAO16B integrated with disulfide bonds, can efficiently deliver Cas9 mRNA and sgRNA into cells while releasing RNA in response to the reductive intracellular environment for genome editing. BAMEAO16B can be used for the research of gene editing.

For research use only. We do not sell to patients.

BAMEA-O16B Chemical Structure

BAMEA-O16B Chemical Structure

CAS No. : 2490668-30-7

Size Price Stock Quantity
50 mg USD 950 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

BAMEAO16B is a lipid nanoparticle. BAMEAO16B integrated with disulfide bonds, can efficiently deliver Cas9 mRNA and sgRNA into cells while releasing RNA in response to the reductive intracellular environment for genome editing. BAMEAO16B can be used for the research of gene editing[1].

In Vitro

BAMEA-O16B/Cas9 mRNA/sgHPV18 (HeLa cells) treatment significantly prohibits HeLa growth compared to that of a scramble sgRNA and Cas9 mRNA delivery. BAMEA-O16B shows RNA delivery efficiency. BAMEA-O16B shows mRNA encapsulation efficiency. BAMEA-O16B shows GFP knockout efficiency. BAMEA-O16B mediated Cas9mRNA delivery is able to regulate endogenous gene expression. BAMEA-O16B/RNA treated cells shows a higher endosome escape efficiency than that of BAMEA-O16/RNA treatment. BAMEA-O16B/RFP mRNA (HeLa cells) nanoparticles results in efficient RFP expression[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

BAMEA-O16B/Cas9 mRNA/sgRNA (I.v.) nanoparticle effectively knocks mouse serum proprotein convertase subtilisin/kexin type 9 (PCSK9) level down to 20% of nontreated mouse. BAMEA-O16B/Cas9 mRNA/sgPCSK9 nanoparticle reduces mouse serum PCSK9 down to 20% of that with DPBS injection or BAMEA-O16B/Cas9 mRNA/scramblesgRNA nanoparticle injections[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1114.89

Formula

C₅₆H₁₁₁N₃O₆S₆

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

Ethanol : 100 mg/mL (89.69 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.8969 mL 4.4847 mL 8.9695 mL
5 mM 0.1794 mL 0.8969 mL 1.7939 mL
10 mM 0.0897 mL 0.4485 mL 0.8969 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (2.24 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% EtOH    90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (2.24 mM); Suspended solution; Need ultrasonic

  • 3.

    Add each solvent one by one:  10% EtOH    90% corn oil

    Solubility: ≥ 2.5 mg/mL (2.24 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Product Name:
BAMEA-O16B
Cat. No.:
HY-139306
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