Basic Blue 20

Cat. No.: HY-W110781: FAQs
Data Sheet Handling Instructions

Molarity Calculator

Basic Blue 20 is a very convenient red-emitting DNA stains. Basic Blue 20 has relatively narrow excitation and emission spectra, with peaks at 633 and 677 nm, respectively. Basic Blue 20 also has a very high resistance to photobleaching.

For research use only. We do not sell to patients.

Basic Blue 20 Chemical Structure

CAS No. : 82-94-0

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Other size		Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Molecular Weight

458.47

Formula

C₂₆H₃₃Cl₂N₃

CAS No.

82-94-0

Appearance

Solid

Color

Brown to black

SMILES

C[N+](C)(C)C1=CC=C(/C(C2=CC=C(N(C)C)C=C2)=C3C=C/C(C=C\3)=[N+](C)/C)C=C1.[Cl-].[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (272.65 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 4.72 mg/mL (10.30 mM; ultrasonic and warming and adjust pH to 9 with HCl and heat to 80°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1812 mL	10.9058 mL	21.8117 mL
5 mM	0.4362 mL	2.1812 mL	4.3623 mL
10 mM	0.2181 mL	1.0906 mL	2.1812 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.54 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.54 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Prieto D, et al. A fast, low cost, and highly efficient fluorescent DNA labeling method using methyl green. Histochem Cell Biol. 2014;142(3):335-345. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.1812 mL	10.9058 mL	21.8117 mL	54.5292 mL
	5 mM	0.4362 mL	2.1812 mL	4.3623 mL	10.9058 mL
	10 mM	0.2181 mL	1.0906 mL	2.1812 mL	5.4529 mL
DMSO	15 mM	0.1454 mL	0.7271 mL	1.4541 mL	3.6353 mL
	20 mM	0.1091 mL	0.5453 mL	1.0906 mL	2.7265 mL
	25 mM	0.0872 mL	0.4362 mL	0.8725 mL	2.1812 mL
	30 mM	0.0727 mL	0.3635 mL	0.7271 mL	1.8176 mL
	40 mM	0.0545 mL	0.2726 mL	0.5453 mL	1.3632 mL
	50 mM	0.0436 mL	0.2181 mL	0.4362 mL	1.0906 mL
	60 mM	0.0364 mL	0.1818 mL	0.3635 mL	0.9088 mL
	80 mM	0.0273 mL	0.1363 mL	0.2726 mL	0.6816 mL
	100 mM	0.0218 mL	0.1091 mL	0.2181 mL	0.5453 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Basic Blue 20 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Basic Blue 2082-94-0Basic Blue20Basic Blue-20DNA Stainred-emittingDNAnarrowexcitationemissionphotobleachingInhibitorinhibitorinhibit

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