BAY-8400
Based on 3 publication(s) in Google Scholar
BAY-8400 is an orally active, potent and selective DNA-dependent protein kinase (DNA-PK) inhibitor (IC50=81 nM). BAY-8400 can be used for the research of cancer.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.38%
- No. CAS: 2763602-59-9
- Fòrmula: C21H17F2N5O
- Peso molecular:393.39
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) BAY-8400
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Actividad biológica
Descripciòn
IC50 & Target
IC50: 81 nM (DNA-PK)[1]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HT-29 | IC50 |
2540 nM
Compound: 25; BAY-8400
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Antiproliferative activity against human HT-29 cells incubated for 4 days by crystal violet staining based assay
Antiproliferative activity against human HT-29 cells incubated for 4 days by crystal violet staining based assay
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[PMID: 34428039] |
| HT-29 | IC50 |
358 nM
Compound: 25; BAY-8400
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Antiproliferative activity against human HT-29 cells incubated for 4 days in presence of bleomycin by crystal violet staining based assay
Antiproliferative activity against human HT-29 cells incubated for 4 days in presence of bleomycin by crystal violet staining based assay
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[PMID: 34428039] |
| SU-DHL-8 | IC50 |
9 nM
Compound: 25; BAY-8400
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Antiproliferative activity against human SU-DHL-8 cells incubated for 4 days by crystal violet staining based assay
Antiproliferative activity against human SU-DHL-8 cells incubated for 4 days by crystal violet staining based assay
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[PMID: 34428039] |
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 2763602-59-9
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Appearance Solid
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Peso molecular 393.39
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Fòrmula C21H17F2N5O
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Color Off-white to light yellow
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SMILES
FC(C1=CC(C2=CC=C3N=CC4=NN=C(N4C3=C2)C5=CCCOCC5)=CN=C1)F
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
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Journal Impact Factor
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Most Recent
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Nat Biotechnol
High-efficiency transgene integration by homology-directed repair in human primary cells using DNA-PKcs inhibition. [Abstract]2024 May;42(5):731-744. PMID: 37537500 -
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Solvente y solubilidad
In Vitro:
DMSO : 62.5 mg/mL (158.88 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.29 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (276 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5420 mL | 12.7100 mL | 25.4201 mL | 63.5502 mL |
| 5 mM | 0.5084 mL | 2.5420 mL | 5.0840 mL | 12.7100 mL | |
| 10 mM | 0.2542 mL | 1.2710 mL | 2.5420 mL | 6.3550 mL | |
| 15 mM | 0.1695 mL | 0.8473 mL | 1.6947 mL | 4.2367 mL | |
| 20 mM | 0.1271 mL | 0.6355 mL | 1.2710 mL | 3.1775 mL | |
| 25 mM | 0.1017 mL | 0.5084 mL | 1.0168 mL | 2.5420 mL | |
| 30 mM | 0.0847 mL | 0.4237 mL | 0.8473 mL | 2.1183 mL | |
| 40 mM | 0.0636 mL | 0.3178 mL | 0.6355 mL | 1.5888 mL | |
| 50 mM | 0.0508 mL | 0.2542 mL | 0.5084 mL | 1.2710 mL | |
| 60 mM | 0.0424 mL | 0.2118 mL | 0.4237 mL | 1.0592 mL | |
| 80 mM | 0.0318 mL | 0.1589 mL | 0.3178 mL | 0.7944 mL | |
| 100 mM | 0.0254 mL | 0.1271 mL | 0.2542 mL | 0.6355 mL |
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