Beta-asarone
Based on 4 publication(s) in Google Scholar
Beta-asarone is an orally active and BBB-penetrable anti-inflammatory agent and neuroprotective agent, which is the major ingredient of Acorus tatarinowii Schott. Beta-asarone can protect nerve cells from apoptosis and autophagy, inhibit expression of α-synuclein, as well as myocardial protection. Beta-asarone can be used in the study of neurological and cardiovascular diseases.
For research use only. We do not sell to patients.
- Purity : 99.96%
- CAS No.: 5273-86-9
- Formula: C12H16O3
- Molecular Weight:208.25
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Beta-asarone
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Customer Validation & Images
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Cell Proliferation/Viability Assay
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WB
All α-synuclein Isoforms
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Biological Activity
Description
IC50 & Target
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α-synuclein |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| HeLa | IC50 |
126.11 μM
Compound: A, beta-Asarone
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Cytotoxicity against human HeLa cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human HeLa cells assessed as growth inhibition after 72 hrs by MTT assay
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[PMID: 23395966] |
| MCF7 | IC50 |
129.35 μM
Compound: A, beta-Asarone
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Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTT assay
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[PMID: 23395966] |
| Oocyte | EC50 |
171.5 μM
Compound: 1
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Allosteric modulation of rat gamma-aminobutyric acid receptor A alpha1beta2gamma2S expressed in Xenopus oocytes assessed as potentiation of GABA-mediated chloride current after 20 secs by voltage clamp assay
Allosteric modulation of rat gamma-aminobutyric acid receptor A alpha1beta2gamma2S expressed in Xenopus oocytes assessed as potentiation of GABA-mediated chloride current after 20 secs by voltage clamp assay
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[PMID: 21563811] |
| SW982 | IC50 |
>150 μM
Compound: A, beta-Asarone
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Cytotoxicity against human SW982 cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human SW982 cells assessed as growth inhibition after 72 hrs by MTT assay
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[PMID: 23395966] |
In Vitro
Beta-asarone (7.5-30 μg/mL; 25 h) can protect PC12 cells against Aβ1-42 induced cytotoxicity and autophagy[1].
Beta-asarone (100 μM; 2 h) inhibits the up-regulation of MALAT1 and α-synuclein expression in SH-SY5Y cells treated with MPP+ (HY-W008719)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:MPP+ (HY-W008719) treated SH-SY5Y cells
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Concentration:100 μM
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Incubation Time:2 h
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Result:Down-regulated the mRNA levels of MALAT1 and α-synuclein.
In Vivo
Beta-asarone (oral administration; 12.5-50 mg/kg; 28 days) alleviates Aβ1–42-induced apoptosis of hippocampal neurons in a rat model of Alzheimer's disease[3].
Beta-asarone (intraperitoneal injection; 10-30 mg/kg; single dose) has anti-inflammatory and protective effects in a rat model of myocardial ischemia-reperfusion[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:MPTP (HY-15608) treated male C57BL/6 mice aged 9-10 weeks old[2]
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Dosage:10 mg/kg
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Administration:Intragastric administration; 28 days
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Result:Markedly increased the TH+ cells of MPTP induced PD mouse.
Down-regulated the expression levels of MALAT1 and α-synuclein in midbrain.
Increased the expression levels of Bcl-2.
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Animal Model:Ischemic-reperfusion treated male Sprague-Dawley rats (240-280g)[4]
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Dosage:10, 20 and 30 mg/kg
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Administration:Intraperitoneal injection (i.p.); single dose
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Result:Significantly improved the heart outcome after myocardial ischemia and reperfusion in terms of less infarction size and lower serum cTNT concentration.
Evidently inhibited inflammatory response with less granulocyte infiltration, mild tissue edema and lower tissue MPO content.
Suppressed NLRP3 signal pathway and cardiac cell’s pyroptosis for less protein expressions of ASC and NLRP3, lower level cleavage activation of caspase-1and GSDMSD, and lower serum IL-1β concentration.
Well preserved the left ventricular performance with higher ejection fraction and fractional shortening.
Chemical Information
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CAS No. 5273-86-9
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Appearance Liquid (Density: 1.073 g/cm3)
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Molecular Weight 208.25
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Formula C12H16O3
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Color Colorless to light yellow
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SMILES
C/C=C\C1=C(OC)C=C(OC)C(OC)=C1
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (4)
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Journal Impact Factor
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Most Recent
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Eur J Pharmacol
Beta-asarone alleviated cerebral ischemia/reperfusion injury by targeting PINK1/Parkin-dependent mitophagy. [Abstract]2025 Sep 5:1002:177831. PMID: 40490171 -
Cancer Med
β-asarone inhibits the migration, invasion, and EMT of bladder cancer through activating ER stress. [Abstract]2023 Jun;12(12):13610-13622. PMID: 37306628 -
Biochem Genet
β-Asarone Inhibits Carboplatin Resistance in Retinoblastoma Cells Through the UCA1/miR-206/NRP1 Axis. [Abstract]2024 Dec 24. PMID: 39718722 -
Int Ophthalmol
β-asarone attenuates the proliferation, migration and enhances apoptosis of retinoblastoma through Wnt/β-catenin signaling pathway. [Abstract]2023 May;43(5):1687-1699. PMID: 36372820
Beta-asarone purchased from MedChemExpress. Usage Cited in: Int Ophthalmol. 2023 May;43(5):1687-1699. [Abstract]
Beta-asarone (β-asarone; 100 μM; 24 h) signifcantly reduces the proliferation of RB cells.
Beta-asarone purchased from MedChemExpress. Usage Cited in: Int Ophthalmol. 2023 May;43(5):1687-1699. [Abstract]
Beta-asarone (β-asarone; 100 μM; 4 h) facilitates the apoptosis of RB cells.
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (480.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.75 mg/mL (13.21 mM); Clear solution
This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.75 mg/mL (13.21 mM); Clear solution
This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocols
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Apoptosis
Apoptosis, also called programmed cell death, is generally characterized by distinct morphological characteristics.
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TUNEL staining for apoptotic DNA fragmentation
TUNEL staining detects DNA strand breaks by using terminal deoxynucleotidyl transferase to add labeled nucleotides to exposed 3′-OH DNA termini, generating either microscopic staining in fixed cells or tissue sections, or fluorescence/cytometric signal in cell suspensions. TUNEL positivity reflects DNA fragmentation but should not be interpreted alone as definitive apoptosis, because TUNEL can also label necrotic, autolytic, mechanically damaged, or DNA-repair-associated DNA breaks.
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Annexin V plus membrane-impermeant dye apoptosis staining
Annexin V-based apoptosis assays rely on the detection of phosphatidylserine (PS) externalization from the inner leaflet of the plasma membrane to the outer leaflet, an early biochemical hallmark of apoptosis. Fluorescently labeled Annexin V binds PS in a calcium-dependent manner, enabling identification of early apoptotic cells by flow cytometry or fluorescence microscopy. When combined with a membrane-impermeant DNA-binding dye (e. g. , propidium iodide), this approach allows discrimination between viable (Annexin V−/dye−), early apoptotic (Annexin V+/dye−), and late apoptotic or necrotic (Annexin V+/dye+) cell populations by assessing membrane integrity and PS exposure.
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Autophagy
Autophagy is a process in which eukaryotic cells use lysosomes to degrade their own cytoplasmic proteins and damaged organelles under the regulation of autophagy related gene (Atg). Microtubule-associated proteins light chain 3 (LC3) is recognized as autophagy marker, which transfers from cytoplasmic LC3 (LC3-I) to membrane type (LC3-II). LC3-II/I ratio could be detected by Western Blot and fluorescence microscopy.
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Apoptosis Solutions
Apoptosis is a regulated, generally non-lytic cell-death pathway that removes unwanted, damaged, infected, or abnormal cells through coordinated morphological changes, caspase activation, DNA fragmentation, and membrane remodeling. The intrinsic apoptosis pathway is controlled mainly by mitochondrial outer membrane permeabilization, BCL-2 family proteins, cytochrome c release, apoptosome formation, caspase-9 activation, and downstream executioner caspase-3/7 activation. The extrinsic apoptosis pathway is initiated by death receptors such as Fas, TNFR, and TRAIL receptors, which recruit adaptor proteins and activate caspase-8 before engaging executioner caspases or mitochondrial amplification through BID cleavage. Apoptosis is linked to many phenotypes, including cancer cell killing, tissue homeostasis, immune regulation, neurodegeneration, infection response, and treatment-induced cytotoxicity; unresolved questions include how apoptosis interacts with necroptosis, pyroptosis, ferroptos
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
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Lysosome and acidic-vesicle live-cell staining
Lysosome and acidic-vesicle live-cell staining detects acidic intracellular compartments by using membrane-permeant acidotropic probes that accumulate in low-pH vesicles, including lysosomes, late endosomes, autolysosomes, and acidic phagosomes. LysoTracker staining is commonly used as an intensity-based readout of acidic lysosomal compartment abundance or enlargement, while acridine orange produces green fluorescence in less concentrated compartments and red fluorescence after concentration-dependent accumulation in acidic vesicular organelles. Loss or reduction of acridine-orange red signal can be used as a readout of lysosomal membrane permeabilization or reduced acidic-vesicle integrity. This protocol is designed for live cultured cells and can be adapted for fluorescence microscopy, high-content imaging, plate-reader readout, or flow cytometry when the selected literature supports the readout. Because these dyes report acidotropic accumulation rather than lysosome identity alone,
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Macroautophagy Solutions
Macroautophagy is a conserved lysosome-dependent degradation pathway in which cytoplasmic material is sequestered into double-membrane autophagosomes and delivered to lysosomes for degradation and recycling. The pathway supports cellular homeostasis during nutrient limitation, organelle stress, protein-aggregate accumulation, infection, differentiation, and tissue remodeling by coupling cargo sequestration, autophagosome maturation, lysosomal fusion, and degradation of cargo-derived macromolecules. The core molecular sequence includes initiation by nutrient- and stress-regulated autophagy machinery, autophagosome nucleation, LC3/ATG8-family conjugation to autophagosomal membranes, cargo selection through receptors such as SQSTM1/p62, autophagosome-lysosome fusion, and lysosomal degradation. LC3 was identified as a mammalian homolog of yeast Atg8 that localizes to autophagosomal membranes after processing, and p62/SQSTM1 was shown to connect ubiquitinated cargo with autophagic degradati
Purity & Documentation
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Data Sheet (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Xue Z, et al. Beta-asarone attenuates amyloid beta-induced autophagy via Akt/mTOR pathway in PC12 cells. Eur J Pharmacol. 2014 Oct 15;741:195-204. [Content Brief]
[2]. Zhang QS, et al. Beta-asarone protects against MPTP-induced Parkinson's disease via regulating long non-coding RNA MALAT1 and inhibiting α-synuclein protein expression. Biomed Pharmacother. 2016 Oct;83:153-159. [Content Brief]
[3]. Liu J, et al. Beta-asarone attenuates neuronal apoptosis induced by Beta amyloid in rat hippocampus. Yakugaku Zasshi. 2010 May;130(5):737-46. [Content Brief]
[4]. Xiao B, et al. Beta-Asarone Alleviates Myocardial Ischemia-Reperfusion Injury by Inhibiting Inflammatory Response and NLRP3 Inflammasome Mediated Pyroptosis. Biol Pharm Bull. 2020 Jul 1;43(7):1046-1051. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 4.8019 mL | 24.0096 mL | 48.0192 mL | 120.0480 mL |
| 5 mM | 0.9604 mL | 4.8019 mL | 9.6038 mL | 24.0096 mL | |
| 10 mM | 0.4802 mL | 2.4010 mL | 4.8019 mL | 12.0048 mL | |
| 15 mM | 0.3201 mL | 1.6006 mL | 3.2013 mL | 8.0032 mL | |
| 20 mM | 0.2401 mL | 1.2005 mL | 2.4010 mL | 6.0024 mL | |
| 25 mM | 0.1921 mL | 0.9604 mL | 1.9208 mL | 4.8019 mL | |
| 30 mM | 0.1601 mL | 0.8003 mL | 1.6006 mL | 4.0016 mL | |
| 40 mM | 0.1200 mL | 0.6002 mL | 1.2005 mL | 3.0012 mL | |
| 50 mM | 0.0960 mL | 0.4802 mL | 0.9604 mL | 2.4010 mL | |
| 60 mM | 0.0800 mL | 0.4002 mL | 0.8003 mL | 2.0008 mL | |
| 80 mM | 0.0600 mL | 0.3001 mL | 0.6002 mL | 1.5006 mL | |
| 100 mM | 0.0480 mL | 0.2401 mL | 0.4802 mL | 1.2005 mL |
Beta-asarone Related Classifications
HY-N1501 Related Classifications
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