BI-0319

BI-0319 (0.1 nM-100 µM; 6-21 days) exerts weak inhibitory effects on anchorage-independent growth in 11 HCC cell lines, and induces sustained degradation of PTK2 protein in HSC-3, C32, CFPAC-1 and A549 cells, but shows no significant antiproliferative activity^[1].
BI-0319 (10 nM-25 µM; 18 h) induces dose-dependent degradation of PTK2 protein in A549 cells (with a DC₅₀ of 243 nM) and 11 HCC cell lines^[1].
BI-0319 (3 μM; 18 h) exerts highly selective degradation of PTK2 protein and off-target degradation of PDE6D protein in A549 cells^[1].
BI-0319 (10 nM-3.16 µM; 18-72 h) exerts a time-dependent slow degradation effect on PTK2 protein in Hep3B2.1-7 cells, and this degradation capacity is significantly enhanced when combined with 10 μM Cyclosporin A (HY-B0579) (pDC₅₀ = 7.4, D_max = 88%)^[1].
BI-0319 inhibits the activity of the purified human PTK2 kinase domain with an IC₅₀ of 19 nM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.