Biotin-11-dCTP 

Biotin-11-dCTP is a biotinylated deoxynucleoside triphosphate and an important DNA labeling reagent. In random primer DNA labeling reactions, Biotin-11-dCTP incorporates into newly synthesized DNA strands to generate labeled DNA probes suitable for hybridization applications. In addition, Biotin-11-dCTP can serve as a substrate for terminal deoxynucleotidyl transferase to end-label oligonucleotides for telomere sequence detection, or to label the cut ends of linearized DNA molecules, thereby supporting streptavidin-based electron microscopy analysis. For example, Biotin-11-dCTP can label the cut ends of linearized DNA molecules under the action of dGTP and avian myeloblastosis virus reverse transcriptase^[1][2].

Biotin-11-dCTP (200-fold excess over oligomer 3' ends) enables effective end-labeling of (GGGTTA)₇ and (TAACCC)₇ oligomers, which then hybridize to telomeric sequences in human, Chinese hamster, and rat chromosomes^[1].
Biotin-11-dCTP (0.02 mM; 45 min at 37°C) effectively labels linearized hemimethylated circular G-T heteroduplex DNA termini to enable mapping of mismatch repair excision tracts via electron microscopy^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

879.49

C₂₈H₄₃Li₃N₇O₁₆P₃S

Liquid

Colorless to light yellow

[H][C@@]1(NC(N2)=O)[C@@]2([H])[C@H](CCCCC(NCCCCCC(NCC#CC(C(N)N3)=CN([C@H]4C[C@H](O)[C@@H](COP(OP(OP(O)(O[Li])=O)(O[Li])=O)(O[Li])=O)O4)C3=O)=O)=O)SC1

Room temperature in continental US; may vary elsewhere.

Solution, -20°C, protect from light, 2 years

• [1]. Moyzis RK, et al. A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes. Proc Natl Acad Sci U S A. 1988;85(18):6622-6626.  [Content Brief]

  [2]. Grilley M, et al. Bidirectional excision in methyl-directed mismatch repair. J Biol Chem. 1993;268(16):11830-11837.  [Content Brief]