Biotin-11-dCTP
Based on 1 publication(s) in Google Scholar
Biotin-11-dCTP is a biotinylated deoxynucleoside triphosphate and an important DNA labeling reagent. In random primer DNA labeling reactions, Biotin-11-dCTP incorporates into newly synthesized DNA strands to generate labeled DNA probes suitable for hybridization applications. In addition, Biotin-11-dCTP can serve as a substrate for terminal deoxynucleotidyl transferase to end-label oligonucleotides for telomere sequence detection, or to label the cut ends of linearized DNA molecules, thereby supporting streptavidin-based electron microscopy analysis. For example, Biotin-11-dCTP can label the cut ends of linearized DNA molecules under the action of dGTP and avian myeloblastosis virus reverse transcriptase.
For research use only. We do not sell to patients.
- Purity: 99.1%
- Formula: C28H43Li3N7O16P3S
- Molecular Weight:879.49
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Storage:
Solution, -20°C, protect from light, 2 years
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Biotin-11-dCTP
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Biological Activity
Biotin-11-dCTP (200-fold excess over oligomer 3' ends) enables effective end-labeling of (GGGTTA)7 and (TAACCC)7 oligomers, which then hybridize to telomeric sequences in human, Chinese hamster, and rat chromosomes[1].
Biotin-11-dCTP (0.02 mM; 45 min at 37°C) effectively labels linearized hemimethylated circular G-T heteroduplex DNA termini to enable mapping of mismatch repair excision tracts via electron microscopy[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Liquid
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Molecular Weight 879.49
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Formula C28H43Li3N7O16P3S
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Color Colorless to light yellow
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SMILES
[H][C@@]1(NC(N2)=O)[C@@]2([H])[C@H](CCCCC(NCCCCCC(NCC#CC(C(N)N3)=CN([C@H]4C[C@H](O)[C@@H](COP(OP(OP(O)(O[Li])=O)(O[Li])=O)(O[Li])=O)O4)C3=O)=O)=O)SC1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Solution, -20°C, protect from light, 2 years
Publications (1)
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Journal Impact Factor
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Most Recent
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Biol Methods Protoc
2026 Apr 15;11(1):bpag019. PMID: 42112493
Purity & Documentation
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Data Sheet (266 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Moyzis RK, et al. A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes. Proc Natl Acad Sci U S A. 1988;85(18):6622-6626. [Content Brief]
[2]. Grilley M, et al. Bidirectional excision in methyl-directed mismatch repair. J Biol Chem. 1993;268(16):11830-11837. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Biotin-11-dCTP Related Classifications
HY-D1668 Related Classifications
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- Biotin-11-dCTP
- DNA Stain
- Reverse Transcriptase
- DNA labeling agent
- avian myeloblastosis virus reverse transcriptase
- random-primed DNA labeling reactions
- cytosine
- terminal deoxynucleotidyltransferase
- telomeric sequences
- DNA probes
- metaphase chromosomes
- DNA strands
- deoxynucleoside triphosphate
- Inhibitor
- inhibitor
- inhibit