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Referencia número: HY-D1668

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Biotin-11-dCTP

Name: Biotin-11-dCTP
Brand: MedChemExpress
SKU: HY-D1668
Price: 550.00 USD
Availability: InStock
Rating: 5 (1 reviews)

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

Biotin-11-dCTP is a biotinylated deoxynucleoside triphosphate and an important DNA labeling reagent. In random primer DNA labeling reactions, Biotin-11-dCTP incorporates into newly synthesized DNA strands to generate labeled DNA probes suitable for hybridization applications. In addition, Biotin-11-dCTP can serve as a substrate for terminal deoxynucleotidyl transferase to end-label oligonucleotides for telomere sequence detection, or to label the cut ends of linearized DNA molecules, thereby supporting streptavidin-based electron microscopy analysis. For example, Biotin-11-dCTP can label the cut ends of linearized DNA molecules under the action of dGTP and avian myeloblastosis virus reverse transcriptase.

Para uso exclusivo en investigación. No vendemos a pacientes.

Pureza: 99.1%
Fòrmula: C₂₈H₄₃Li₃N₇O₁₆P₃S
Peso molecular:879.49

Almacenamiento:

Solution, -20°C, protect from light, 2 years

Biotin-11-dCTP Chemical Structure

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

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Chemical Information
Publications (1)
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Actividad biológica

Descripciòn

In Vitro

Biotin-11-dCTP (200-fold excess over oligomer 3' ends) enables effective end-labeling of (GGGTTA)₇ and (TAACCC)₇ oligomers, which then hybridize to telomeric sequences in human, Chinese hamster, and rat chromosomes^[1].
Biotin-11-dCTP (0.02 mM; 45 min at 37°C) effectively labels linearized hemimethylated circular G-T heteroduplex DNA termini to enable mapping of mismatch repair excision tracts via electron microscopy^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

Appearance Liquid
Peso molecular 879.49
Fòrmula C₂₈H₄₃Li₃N₇O₁₆P₃S
Color Colorless to light yellow
SMILES

[H][C@@]1(NC(N2)=O)[C@@]2([H])[C@H](CCCCC(NCCCCCC(NCC#CC(C(N)N3)=CN([C@H]4C[C@H](O)[C@@H](COP(OP(OP(O)(O[Li])=O)(O[Li])=O)(O[Li])=O)O4)C3=O)=O)=O)SC1
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

Solution, -20°C, protect from light, 2 years

Publications (1)

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Biol Methods Protoc

A low-input Micro-C protocol for high-resolution 3D genome mapping. [Abstract]

2026 Apr 15;11(1):bpag019. PMID: 42112493

Pureza y Documentación

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Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (266 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Moyzis RK, et al. A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes. Proc Natl Acad Sci U S A. 1988;85(18):6622-6626. [Content Brief]

[2]. Grilley M, et al. Bidirectional excision in methyl-directed mismatch repair. J Biol Chem. 1993;268(16):11830-11837. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Biotin-11-dCTP

1 Cited Publications

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Actividad biológica
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Biotin-11-dCTP

Publications Citing Use of MedChemExpress (MCE) Biotin-11-dCTP