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Cat. No.: HY-150048

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BK50164

BK50164 is a potent CD73 inhibitor with an IC₅₀ value of 13.089 μM. BK50164 binds to CD99 with a K_D value of 1.5 μM. BK50164 shows antiproliferative activity. BK50164 induced Apoptosis and cell cycle arrest at Sub-G1 phase.

For research use only. We do not sell to patients.

CAS No.: 2204291-78-9
Formula: C₁₃H₁₃ClFN₅O₇
Molecular Weight:405.72

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (274 KB)

BK50164 Chemical Structure

CAS No.: 2204291-78-9

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Antibody-Drug Conjugates

One-stop solution for XDC (ADC/PDC/RDC/ApDC/FDC/ISAC/VDC/SMDC)

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

IC₅₀: 13.089 µM (CD73)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
143B	IC₅₀	>341 μM Compound: BK50164	Cytotoxicity against human 143B cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human 143B cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
A 172	IC₅₀	>341 μM Compound: BK50164	Cytotoxicity against human A-172 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human A-172 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
A549	IC₅₀	>400 μM Compound: BK50164	Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
HEK293	IC₅₀	>341 μM Compound: BK50164	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
HeLa	IC₅₀	>400 μM Compound: BK50164	Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
HOS	IC₅₀	>400 μM Compound: BK50164	Cytotoxicity against human HOS cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human HOS cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
PC-3	IC₅₀	>400 μM Compound: BK50164	Cytotoxicity against human PC-3 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human PC-3 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
RD	IC₅₀	>400 μM Compound: BK50164	Cytotoxicity against human RD cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human RD cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
SAOS-2	IC₅₀	>=400 μM Compound: BK50164	Cytotoxicity against human SAOS-2 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human SAOS-2 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
SJRH30	IC₅₀	>341 μM Compound: BK50164	Cytotoxicity against human Rh30 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human Rh30 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
TC-32	IC₅₀	27 μM Compound: BK50164	Cytotoxicity against human TC-32 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human TC-32 cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
U2OS	IC₅₀	>=400 μM Compound: BK50164	Cytotoxicity against human U2OS cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human U2OS cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]
U-87MG ATCC	IC₅₀	>400 μM Compound: BK50164	Cytotoxicity against human U-87 MG cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay Cytotoxicity against human U-87 MG cells assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Blue reagent based luminescence assay	[PMID: 36917882]

In Vitro

BK50164 (0-400 µM; 48 h) shows antiproliferative activity with IC₅₀s of 35.8, 34.28, 5.17, ≥400, ≥400 µM for A4573, TC-32, TC-71, Saos-2, U-2 OS cells, respectively^[2].
BK50164 (4-16 µM; 48 h) induces apoptosis and cell cycle arrest at Sub-G1 phase^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

A4573, TC-32, TC-71, Saos-2, U-2 OS cells
Concentration:

0-400 µM
Incubation Time:

48 h
Result:

Inhibited cell viability witn IC₅₀s of 35.8, 34.28, 5.17, ≥400, ≥400 µM for A4573, TC-32, TC-71, Saos-2, U-2 OS cells, respectively.

Cell Cycle Analysis^[2]

Cell Line:

TC-32, U-2 OS cells
Concentration:

4, 8, 16 µM
Incubation Time:

48 h
Result:

Induced apoptosis in only TC-32 ES cells but not in U-2 OS cell line and induces cell cycle arrest at Sub-G1 phase.

Chemical Information

CAS No. 2204291-78-9
Molecular Weight 405.72
Formula C₁₃H₁₃ClFN₅O₇
SMILES

NC1=NC(Cl)=NC2=C1N=CN2[C@@H]3O[C@H](COC(C(O)=O)C(O)=O)[C@@H](O)[C@@H]3F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Roland Joseph Billedeau, et al. Ectonucleotidase inhibitors and methods of use thereof. WO2018119284A1.

[2]. Balaraman K, et al. Design, synthesis and biological evaluation of Nucleosidic CD99 inhibitors that selectively reduce Ewing sarcoma viability. Eur J Med Chem. 2023 May 5;251:115244. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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