BN-82685
Based on 1 Customer Validation
BN-82685 is a a quinone-based CDC25 inhibitor with IC50 of 201 nM, 117 nM, 109 nM, 160 nM, 249 nM, for CDC25C, CDC25C-cat, CDC25A, CDC25B2, CDC25B3, respectively. BN-82685 plays an important role in cancer research.
For research use only. We do not sell to patients.
- Purity: 95.46%
- CAS No.: 477603-18-2
- Formula: C12H15N3O2S
- Molecular Weight:265.33
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| DU-145 | IC50 |
0.11 μM
Compound: 2a, BN82685
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Antiproliferative activity against human androgen-independent prostate carcinoma DU145 cell line by colorimetric assay
Antiproliferative activity against human androgen-independent prostate carcinoma DU145 cell line by colorimetric assay
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[PMID: 16216500] |
| MIA PaCa-2 | IC50 |
0.18 μM
Compound: 2a, BN82685
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Antiproliferative activity against human pancreatic carcinoma Mia PaCa2 cell line by colorimetric assay
Antiproliferative activity against human pancreatic carcinoma Mia PaCa2 cell line by colorimetric assay
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[PMID: 16216500] |
Chemical Information
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CAS No. 477603-18-2
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Appearance Solid
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Molecular Weight 265.33
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Formula C12H15N3O2S
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Color Brown to red
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SMILES
O=C(C(NCCN(C)C)=CC1=O)C2=C1SC(C)=N2
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Synonyms
IRC-083065
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (376.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.42 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.7689 mL | 18.8445 mL | 37.6889 mL | 94.2223 mL |
| 5 mM | 0.7538 mL | 3.7689 mL | 7.5378 mL | 18.8445 mL | |
| 10 mM | 0.3769 mL | 1.8844 mL | 3.7689 mL | 9.4222 mL | |
| 15 mM | 0.2513 mL | 1.2563 mL | 2.5126 mL | 6.2815 mL | |
| 20 mM | 0.1884 mL | 0.9422 mL | 1.8844 mL | 4.7111 mL | |
| 25 mM | 0.1508 mL | 0.7538 mL | 1.5076 mL | 3.7689 mL | |
| 30 mM | 0.1256 mL | 0.6281 mL | 1.2563 mL | 3.1407 mL | |
| 40 mM | 0.0942 mL | 0.4711 mL | 0.9422 mL | 2.3556 mL | |
| 50 mM | 0.0754 mL | 0.3769 mL | 0.7538 mL | 1.8844 mL | |
| 60 mM | 0.0628 mL | 0.3141 mL | 0.6281 mL | 1.5704 mL | |
| 80 mM | 0.0471 mL | 0.2356 mL | 0.4711 mL | 1.1778 mL | |
| 100 mM | 0.0377 mL | 0.1884 mL | 0.3769 mL | 0.9422 mL |
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