BOP hexafluorophosphate
Based on 1 Customer Validation
BOP hexafluorophosphate is a phosphonium-type condensing agent. BOP hexafluorophosphate activates the carboxylic acid component to form a highly reactive O-benzotriazole ester (active ester) intermediate, which then reacts with the amino component to form an amide bond (peptide bond). BOP hexafluorophosphate is applicable to solid-phase polypeptide synthesis and nucleoside modification.
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- Pureza: 98.0%
- No. CAS: 56602-33-6
- Fòrmula: C12H22F6N6OP2
- Peso molecular:442.28
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Almacenamiento:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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HY-Y0912
HBTU -
HY-Y1703
HATU -
HY-D0178
1-Ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride -
HY-D0177
(Benzotriazol-1-yloxy)tripyrrolidinophosphonium hexafluorophosphate -
HY-Y1092
N,N-Dicyclohexylcarbodiimide(DCC) -
HY-W010951
HCTU -
HY-Y1092R
N,N-Dicyclohexylcarbodiimide(DCC) (Standard) -
HY-Y1168
DMTMM -
HY-W011556
N,N,N',N'-Tetramethylchloroformamidinium hexafluorophosphate -
HY-Z0275
HOAT -
HY-Y1191
EEDQ
Actividad biológica
Descripciòn
In Vitro
The racemization degree of Boc-His (Tos) is minimized when BOP hexafluorophosphate is used with equimolar diisopropylethylamine, and the epimer formation rate reaches approximately 3% upon excessive addition of diisopropylethylamine[1].
BOP hexafluorophosphate activates the amide functional group of silyl-protected pyrimidine nucleosides to generate in situ O4-(benzotriazol-1-yl) derivatives for C4 modification[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 56602-33-6
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Appearance Solid
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Peso molecular 442.28
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Fòrmula C12H22F6N6OP2
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Color White to off-white
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SMILES
F[P-](F)(F)(F)(F)F.CN(C)[P+](N(C)C)(N(C)C)ON1C2=CC=CC=C2N=N1
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (226.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.65 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.65 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (272 KB)
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SDS (604 KB)
- English - EN (604 KB)
- Français - FR (604 KB)
- Deutsch - DE (604 KB)
- Norwegian - NO (604 KB)
- Español - ES (604 KB)
- Swedish - SV (604 KB)
- Italian - IT (604 KB)
- Korean - KR (604 KB)
- Portuguese - PT (604 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Forest M, et al. BOP reagent for the coupling of pGlu and Boc-His(Tos) in solid phase peptide synthesis. Int J Pept Protein Res. 1990;35(2):89-94. [Content Brief]
[2]. Akula HK, et al. Facile Modifications at the C4 Position of Pyrimidine Nucleosides via In Situ Amide Activation with 1H-Benzotriazol-1-yloxy-tris(dimethyl-amino)phosphonium Hexafluorophosphate. Curr Protoc Nucleic Acid Chem. 2019;76(1):e73. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2610 mL | 11.3051 mL | 22.6101 mL | 56.5253 mL |
| 5 mM | 0.4522 mL | 2.2610 mL | 4.5220 mL | 11.3051 mL | |
| 10 mM | 0.2261 mL | 1.1305 mL | 2.2610 mL | 5.6525 mL | |
| 15 mM | 0.1507 mL | 0.7537 mL | 1.5073 mL | 3.7684 mL | |
| 20 mM | 0.1131 mL | 0.5653 mL | 1.1305 mL | 2.8263 mL | |
| 25 mM | 0.0904 mL | 0.4522 mL | 0.9044 mL | 2.2610 mL | |
| 30 mM | 0.0754 mL | 0.3768 mL | 0.7537 mL | 1.8842 mL | |
| 40 mM | 0.0565 mL | 0.2826 mL | 0.5653 mL | 1.4131 mL | |
| 50 mM | 0.0452 mL | 0.2261 mL | 0.4522 mL | 1.1305 mL | |
| 60 mM | 0.0377 mL | 0.1884 mL | 0.3768 mL | 0.9421 mL | |
| 80 mM | 0.0283 mL | 0.1413 mL | 0.2826 mL | 0.7066 mL | |
| 100 mM | 0.0226 mL | 0.1131 mL | 0.2261 mL | 0.5653 mL |
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