BOS-318
Based on 3 publication(s) in Google Scholar
BOS-318 is a potent, slowly dissociating, highly selective, and cell-permeable furin inhibitor with IC50 value of 1.9 nM. BOS-318 protects ENaC from subsequent activation by neutrophil elastase. BOS-318 can be used for the rsearch for CF lung disease.
For research use only. We do not sell to patients.
- Purity: 99.24%
- CAS No.: 2387633-15-8
- Formula: C28H32Cl2N6O3
- Molecular Weight:571.50
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) BOS-318
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Biological Activity
BOS-318 (0.001-1 μM, 72 hours) provides cytoprotection against toxicity in CuFi-1 cells induced by P aeruginosa exotoxin A (PEA)[1].
BOS-318 (U2OS cells) to be approximately 13 times more potent against furin than PCSK5 (IC50 = 25.3 nM), 24 times more potent than PCSK7 (IC50 = 45.8 nM), and 110 times more potent than PCSK6 (IC50 = 209.4 nM) [1].
BOS-318 (0.3 μM, 2 hours or 48 hours) reduces ENaC-mediated Na+ absorption in CF HBECs and protects against NE- and CF sputum-sol-mediated activation of the ENaC[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:CuFi-1 cells
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Concentration:17.4 and 263.0 nM
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Incubation Time:2 and 48 hours
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Result:Significantly inhibited ENaC-mediated Na+ transport as measured in differentiated human bronchial epithelial cells under both short- (IC50= 263.0 nM) and long-term (IC50= 17.4 nM) treatment conditions.
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Cell Line:CuFi-1 cells
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Concentration:0.3 μM
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Incubation Time:72 hours
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Result:Significantly increased cell viability in PEA-treated cells to approximately 88% of control levels.
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Cell Line:CuFi-1 cells
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Concentration:0.001, 0.01, 0.03, 0.1, 0.3 and 1 μM
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Incubation Time:72 hours
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Result:Provided protection against PEA-mediated cytotoxicity in a dose-dependent manner, over a period of 72 h, with an EC50 of 47.8 nM.
Chemical Information
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CAS No. 2387633-15-8
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Appearance Solid
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Molecular Weight 571.50
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Formula C28H32Cl2N6O3
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Color Off-white to light yellow
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SMILES
O=C(O)CC1CCN(CC2=CC(C3=CC(Cl)=CC(Cl)=C3)=NC(OC4=CN=C(N5CCN(C)CC5)N=C4)=C2)CC1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
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Journal Impact Factor
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Most Recent
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Acta Biomater
Fe3+-responsive aggregable gold nanoplatform enables siRNA-mediated chemoresistance reversal and combined photothermal-temozolomide therapy for glioblastoma. [Abstract]2026 Jun 18:S1742-7061(26)00397-1. PMID: 42315000 -
Sci Rep
2025 Aug 1;15(1):28071. PMID: 40751072 -
Npj Viruses
2026 Mar 14;4(1):18. PMID: 41826412
Solvent & Solubility
DMSO : 50 mg/mL (87.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.37 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.37 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Douglas LEJ, et al. A highly selective, cell-permeable furin inhibitor BOS-318 rescues key features of cystic fibrosis airway disease. Cell Chem Biol. 2022 Jun 16;29(6):947-957.e8. [Content Brief]
[2]. Nayak D, et al. Clearing the air: Uniquely engaging furin as an approach to cystic fibrosis therapy. Cell Chem Biol. 2022 Jun 16;29(6):927-929. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7498 mL | 8.7489 mL | 17.4978 mL | 43.7445 mL |
| 5 mM | 0.3500 mL | 1.7498 mL | 3.4996 mL | 8.7489 mL | |
| 10 mM | 0.1750 mL | 0.8749 mL | 1.7498 mL | 4.3745 mL | |
| 15 mM | 0.1167 mL | 0.5833 mL | 1.1665 mL | 2.9163 mL | |
| 20 mM | 0.0875 mL | 0.4374 mL | 0.8749 mL | 2.1872 mL | |
| 25 mM | 0.0700 mL | 0.3500 mL | 0.6999 mL | 1.7498 mL | |
| 30 mM | 0.0583 mL | 0.2916 mL | 0.5833 mL | 1.4582 mL | |
| 40 mM | 0.0437 mL | 0.2187 mL | 0.4374 mL | 1.0936 mL | |
| 50 mM | 0.0350 mL | 0.1750 mL | 0.3500 mL | 0.8749 mL | |
| 60 mM | 0.0292 mL | 0.1458 mL | 0.2916 mL | 0.7291 mL | |
| 80 mM | 0.0219 mL | 0.1094 mL | 0.2187 mL | 0.5468 mL |
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