C12-200

Name: C12-200
Brand: MedChemExpress
SKU: HY-145405
Rating: 5.0 (16 reviews)

Cat. No.: HY-145405 Purity: 99.91%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

C12-200 is a type of ionizable cationic lipid and helper lipid commonly used for mRNA delivery. In the hemophilia B mouse model, mRNA encoding human erythropoietin (EPO) and coagulation factor IX (FIX) can be successfully delivered and expressed using C12-200. C12-200 is utilized in research related to hemophilia B.

For research use only. We do not sell to patients.

CAS No. : 1220890-25-4

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Based on 16 publication(s) in Google Scholar

Other Forms of C12-200:

(S)-C12-200 Get quote
(R)-C12-200 Get quote

16 Publications Citing Use of MCE C12-200

In Vivo Imaging

RT-PCR

: C12-200 purchased from MedChemExpress. Usage Cited in: Adv Mater. 2024 Oct 10:e2409812. [Abstract]; The maintenance of robust luminescence in the lung was observed when the key lipid SM-102 was replaced with either C12-200 or ALC-0315.

: C12-200 purchased from MedChemExpress. Usage Cited in: Immunity. 2023 Jan 10;56(1):180-192.e11. [Abstract]; In hepatoma-bearing mice, intraperitoneal injection of liposomes to specifically deliver IL10RA siRNA to macrophages every 3 days effectively suppressed the expression of IL10RA and CD209B. For developing siRNA-loaded liposomes, a lipid mixture composed of cationic lipid C12-200, cholesterol, DSPC, and mPEG2000-DMG were dissolved in ethanol at a molar ratio of 50:38.5:10:1.5. Thereafter, the siRNA in 10 mM sodium citrate was added to the lipid mixture at a weight ratio of 1 : 7.5 and incubated at 37 ℃ for 30 min. The unentrapped siRNA was removed by ultrafiltration centrifugation. Encapsulation efficiency of the prepared liposomes was over 95%. The siRNA-loaded liposomes were used for in vivo experiments after being diluted with PBS.

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References
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Description

In Vivo

C12-200 (0.25 or 0.5 mg/kg, i.v., single injection) delivers exogenous human mRNA, producing target proteins at supraphysiological levels. Human erythropoietin (EPO) and coagulation factor IX (FIX) demonstrate potent pharmacodynamic effects in the KO mouse model^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Hemophilia B (FIX knockout (KO)) mouse model^[2]
Dosage:	0.25 or 0.5 mg/kg
Administration:	Intravenous injection (i.v.), single injection
Result:	led to plasma FIX levels of 400 ng/ml and 1.77 μg/ml with doses of 0.25 and 0.50 mg/kg, respectively, in KO mice. And C12-200 resulted in a significant increase in activity in the treated mice.

Molecular Weight

1136.93

Formula

C₇₀H₁₄₅N₅O₅

CAS No.

1220890-25-4

Appearance

Liquid (Density: 0.948±0.06 g/cm³)

Color

Colorless to light yellow

SMILES

OC(CCCCCCCCCC)CN(CCN(CC(CCCCCCCCCC)O)CC(CCCCCCCCCC)O)CCN1CCN(CCN(CC(CCCCCCCCCC)O)CC(CCCCCCCCCC)O)CC1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (87.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 100 mg/mL (87.96 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.8796 mL	4.3978 mL	8.7956 mL
5 mM	0.1759 mL	0.8796 mL	1.7591 mL
10 mM	0.0880 mL	0.4398 mL	0.8796 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 4

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 5

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (2.20 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 6

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (244 KB) HNMR (158 KB) RP-HPLC (256 KB) MS (130 KB)

Handling Instructions (2659 KB)

References

[1]. Khare P, et al. Development of Lipidoid Nanoparticles for siRNA Delivery to Neural Cells. AAPS J. 2021;24(1):8. Published 2021 Dec 6. [Content Brief]

[2]. DeRosa F, et al. Therapeutic efficacy in a hemophilia B model using a biosynthetic mRNA liver depot system. Gene Ther. 2016;23(10):699-707. [Content Brief]

[3]. Kauffman KJ, et al. Optimization of Lipid Nanoparticle Formulations for mRNA Delivery in Vivo with Fractional Factorial and Definitive Screening Designs. Nano Lett. 2015 Nov 11;15(11):7300-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / Ethanol	1 mM	0.8796 mL	4.3978 mL	8.7956 mL	21.9890 mL
	5 mM	0.1759 mL	0.8796 mL	1.7591 mL	4.3978 mL
	10 mM	0.0880 mL	0.4398 mL	0.8796 mL	2.1989 mL
	15 mM	0.0586 mL	0.2932 mL	0.5864 mL	1.4659 mL
	20 mM	0.0440 mL	0.2199 mL	0.4398 mL	1.0995 mL
	25 mM	0.0352 mL	0.1759 mL	0.3518 mL	0.8796 mL
	30 mM	0.0293 mL	0.1466 mL	0.2932 mL	0.7330 mL
	40 mM	0.0220 mL	0.1099 mL	0.2199 mL	0.5497 mL
	50 mM	0.0176 mL	0.0880 mL	0.1759 mL	0.4398 mL
	60 mM	0.0147 mL	0.0733 mL	0.1466 mL	0.3665 mL
	80 mM	0.0110 mL	0.0550 mL	0.1099 mL	0.2749 mL

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

C12-2001220890-25-4Liposomelipidoid nanoparticlessiRNA deliveryfactor IXionizable lipidInhibitorinhibitorinhibit

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C12-200

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Other Forms of C12-200:

C12-200 Related Antibodies

16 Publications Citing Use of MCE C12-200

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References

Customer Review

Molarity Calculator

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Complete Stock Solution Preparation Table

C12-200 Related Classifications

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