Methyl nonadecanoate
Based on 1 Customer Validation
Methyl nonadecanoate is an internal standard. Methyl nonadecanoate can be used to determine the content of fatty acid methyl esters (FAME) in biodiesel.
For research use only. We do not sell to patients.
- Purity: 98.54%
- CAS No.: 1731-94-8
- Formula: C20H40O2
- Molecular Weight:312.53
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Methyl nonadecanoate serves as the internal standard for GC-FID-based determination of ester content in tallow biodiesel, which avoids interference from naturally occurring methyl heptadecanoate[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1731-94-8
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Appearance Solid
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Molecular Weight 312.53
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Formula C20H40O2
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Color White to off-white
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SMILES
CCCCCCCCCCCCCCCCCCC(OC)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
Ethanol : 100 mg/mL (319.97 mM; Need ultrasonic)
DMSO : 10 mg/mL (32.00 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (3.20 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 1 mg/mL (3.20 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (265 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Pereira E, et al. Development and validation of analytical methodology by GC-FID using hexadecyl propanoate as an internal standard to determine the bovine tallow methyl esters content. J Chromatogr B Analyt Technol Biomed Life Sci. 2018;1093-1094:134-140. [Content Brief]
[2]. Soares S, et al. A flow-based procedure exploiting the lab-in-syringe approach for the determination of ester content in biodiesel and diesel/biodiesel blends. Talanta. 2017;174:556-561. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO / Ethanol | 1 mM | 3.1997 mL | 15.9985 mL | 31.9969 mL | 79.9923 mL |
| 5 mM | 0.6399 mL | 3.1997 mL | 6.3994 mL | 15.9985 mL | |
| 10 mM | 0.3200 mL | 1.5998 mL | 3.1997 mL | 7.9992 mL | |
| 15 mM | 0.2133 mL | 1.0666 mL | 2.1331 mL | 5.3328 mL | |
| 20 mM | 0.1600 mL | 0.7999 mL | 1.5998 mL | 3.9996 mL | |
| 25 mM | 0.1280 mL | 0.6399 mL | 1.2799 mL | 3.1997 mL | |
| 30 mM | 0.1067 mL | 0.5333 mL | 1.0666 mL | 2.6664 mL | |
| Ethanol | 40 mM | 0.0800 mL | 0.4000 mL | 0.7999 mL | 1.9998 mL |
| 50 mM | 0.0640 mL | 0.3200 mL | 0.6399 mL | 1.5998 mL | |
| 60 mM | 0.0533 mL | 0.2666 mL | 0.5333 mL | 1.3332 mL | |
| 80 mM | 0.0400 mL | 0.2000 mL | 0.4000 mL | 0.9999 mL | |
| 100 mM | 0.0320 mL | 0.1600 mL | 0.3200 mL | 0.7999 mL |
Methyl nonadecanoate Related Classifications
HY-W004262 Related Classifications
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