S19-1035
Based on 1 Customer Validation
S19-1035 is a highly potent and specific aldo-keto reductase 1C3 (AKR1C3) inhibitor. S19-1035 inhibits AKR1C3 with an IC50 value of 3.04 nM. S19-1035 can be used for the research of tumor.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 2986319-18-8
- Formula: C19H17ClN2O3
- Molecular Weight:356.80
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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AKR1C3 3.04 nM (IC50) |
S19-1035 exhibits inhibitory activity for AKR1C3 with an IC50 value of 3.04 nM[1].
S19-1035 (0-100 μM; 72 h or 96 h) has less cytotoxic and had limited antitumor effects when used alone[1].
S19-1035 (10 μM; 8 days) significantly reversed the doxorubicin (DOX) resistance in a resistant breast cancer cell line in co-administration[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MDA-MB-231, MCF-7 and MCF-7/DOX cells
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Concentration:0-100 μM
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Incubation Time:72 h or 96 h
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Result:Had weak antiproliferative effects in all three breast cancer cell lines.
Chemical Information
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CAS No. 2986319-18-8
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Appearance Solid
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Molecular Weight 356.80
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Formula C19H17ClN2O3
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Color White to off-white
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SMILES
CC1=NOC(C)=C1COC2=CC=CC=C2C(NC3=CC=CC=C3Cl)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 125 mg/mL (350.34 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (5.83 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8027 mL | 14.0135 mL | 28.0269 mL | 70.0673 mL |
| 5 mM | 0.5605 mL | 2.8027 mL | 5.6054 mL | 14.0135 mL | |
| 10 mM | 0.2803 mL | 1.4013 mL | 2.8027 mL | 7.0067 mL | |
| 15 mM | 0.1868 mL | 0.9342 mL | 1.8685 mL | 4.6712 mL | |
| 20 mM | 0.1401 mL | 0.7007 mL | 1.4013 mL | 3.5034 mL | |
| 25 mM | 0.1121 mL | 0.5605 mL | 1.1211 mL | 2.8027 mL | |
| 30 mM | 0.0934 mL | 0.4671 mL | 0.9342 mL | 2.3356 mL | |
| 40 mM | 0.0701 mL | 0.3503 mL | 0.7007 mL | 1.7517 mL | |
| 50 mM | 0.0561 mL | 0.2803 mL | 0.5605 mL | 1.4013 mL | |
| 60 mM | 0.0467 mL | 0.2336 mL | 0.4671 mL | 1.1678 mL | |
| 80 mM | 0.0350 mL | 0.1752 mL | 0.3503 mL | 0.8758 mL | |
| 100 mM | 0.0280 mL | 0.1401 mL | 0.2803 mL | 0.7007 mL |
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