Arecoline hydrobromide
Based on 7 publication(s) in Google Scholar
Arecoline hydrobromide, a naturally brain-penetrant and orally active occurring psychoactive alkaloid, is a partial agonist of nicotinic and muscarinic acetylcholine receptor. Arecoline hydrobromide exhibits stimulation, alertness, anxiolysis and anti-parasitic effects. Arecoline hydrobromide also can induce oxidative stress.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté : 99.90%
- CAS No.: 300-08-3
- Formule: C8H14BrNO2
- Masse moléculaire:236.11
-
Stockage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Activité biologique
-
Chemical Information
-
Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
-
Help & FAQs
-
HY-N2364
ArecolinenAChR/mAChR Agonist -
HY-B0710
Betaine -
HY-103124
DOI hydrochloride5-HT2A/5-HT2C Receptor Agonist -
HY-100809
Guvacine hydrochlorideGABA Reuptake Inhibitor -
HY-N5016
Guvacoline hydrochlorideMuscarinic Receptor Full Activator -
HY-N2368A
Arecaidine hydrochlorideGABA transport system Inhibitor -
HY-B0489R
Arecoline hydrobromide (Standard)nAChR/mAChR Agonist -
HY-B0282
Acetylcholine chlorideCholinergic Agonist -
HY-10448
CapsaicinTRPV1 Agonist -
HY-W009724
2-Aminoethyl diphenylborinateIP3R Inhibitor -
HY-N0175
CytisiniclinenAChR Agonist -
HY-B0228
AdenosineEndogenous Autacoid
Publications Citing Use of MedChemExpress (MCE) Arecoline hydrobromide
More- Cardiovasc Diabetol. 2026 Jan 14;25(1):38.
- Ecotoxicol Environ Saf. 2025 Apr 1:294:118108. [Abstract]
- Int J Mol Sci. 2023 Mar 28;24(7):6327. [Abstract]
- Sci Rep. 2026 Mar 31. [Abstract]
- Vet Microbiol. 2026 Jan:312:110818. [Abstract]
- J Oral Pathol Med. 2022 Oct;51(9):780-790. [Abstract]
- SSRN. 2026 Jul 6.
Customer Validation & Images
-
Bio/Physico-chemical Assay
-
Bio/Physico-chemical Assay
-
Cell Imaging/Staining
-
WB
-
IF
Activité biologique
Description
IC50 & Target
nAChR/mAChR[3]
In Vitro
Arecoline hydrobromide induces the generation of reactive oxygen species and cell cycle arrest at the G1/G0 phase in HaCaT cells without affecting the expression of p21/Cip1. Arecoline hydrobromide-induced epithelial cell death at higher concentrations is caused by oxidative trauma without eliciting apoptosis. Arecoline hydrobromide upregulates the expression of the following stress-responsive genes: hemeoxygenase-1; ferritin light chain; glucose-6-phosphate dehydrogenase; glutamatecysteine ligase catalytic subunit; and glutathione reductase[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 300-08-3
-
Appearance Solid
-
Masse moléculaire 236.11
-
Formule C8H14BrNO2
-
Color White to light yellow
-
SMILES
O=C(C1=CCCN(C)C1)OC.Br
-
Synonyms
methyl 1,2,5,6-tetrahydro-1-methyl-3-pyridinecarboxylate hydrobromide
-
Structure Classification
-
Initial Source
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (7)
-
Journal Impact Factor
-
Most Recent
-
-
Ecotoxicol Environ Saf
Assessing the carcinogenic potential and molecular mechanisms of arecoline in human lungs: from in silico methods to in vitro validation. [Abstract]2025 Apr 1:294:118108. PMID: 40147173
Arecoline hydrobromide purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2025 Apr 1:294:118108. [Abstract]
Arecoline exhibited a relatively strong binding affinity to PTGS2 in vitro, with a KD value of 10.47 μM.
-
Int J Mol Sci
Oxidative Stress and Endoplasmic Reticulum Stress Contributes to Arecoline and Its Secondary Metabolites-Induced Dyskinesia in Zebrafish Embryos. [Abstract]2023 Mar 28;24(7):6327. PMID: 37047326 -
Sci Rep
Pomolic acid alleviates CCl4‑induced liver fibrosis in mice by suppressing β-arrestin 2-mediated pro-fibrotic macrophage polarization. [Abstract]2026 Mar 31. PMID: 41917130 -
Vet Microbiol
Mechanistic link between eIF4E phosphorylation and viral pathogenesis: Therapeutic insights from a porcine model. [Abstract]2026 Jan:312:110818. PMID: 41418357
Arecoline hydrobromide purchased from MedChemExpress. Usage Cited in: Vet Microbiol. 2026 Jan:312:110818. [Abstract]
Arecoline (i.p.; single dose) stimulation led to a significant decrease in hepatic and intestinal GSH, SOD, and CAT activities and a significant increase in the MDA content, which were reversed by eFT508 (i.g.; 12 h) administration in BALB/c mice.
-
J Oral Pathol Med
Overexpression of DEC1 in the epithelium of OSF promotes mesenchymal transition via activating FAK/Akt signal axis. [Abstract]2022 Oct;51(9):780-790. PMID: 36053880
Arecoline hydrobromide purchased from MedChemExpress. Usage Cited in: J Oral Pathol Med. 2022 Oct;51(9):780-790. [Abstract]
Cell morphological images of HOKs after treatment with Arecoline (Arecoline hydrobromide; 150 μM; 24 h) and/or hypoxia (scale bar = 100 μm). Several HOKs treated with Arecoline (150 μM) and/or hypoxia (24 h) presented morphological alterations similar to mesenchymal cells, and were most pronounced with combination treatment.
Arecoline hydrobromide purchased from MedChemExpress. Usage Cited in: J Oral Pathol Med. 2022 Oct;51(9):780-790. [Abstract]
Western blot analysis of DEC1 expression in HOKs after treatment with Arecoline (Arecoline hydrobromide; 0-150 μM; 24 h). Arecoline dose-dependently increased DEC1 expression.
Arecoline hydrobromide purchased from MedChemExpress. Usage Cited in: J Oral Pathol Med. 2022 Oct;51(9):780-790. [Abstract]
Fluorescence triple staining of DEC1 (red), phosphorylated FAK (Y397; green), and phosphorylated Akt (Ser473; magenta) in the oral mucosal epithelium of mice after local buccal mucosal injection of Arecoline (Arecoline hydrobromide; 10 mg/kg; once daily) for 2 weeks. Arecoline-induced OSF tissues showed higher DEC1, phosphorylated FAK, and phosphorylated Akt expression than normal oral mucosa.
-
Solvant et solubilité
In Vitro:
H2O : 50 mg/mL (211.77 mM; Need ultrasonic)
DMSO : ≥ 50 mg/mL (211.77 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (8.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (8.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (423.53 mM); Clear solution; Need ultrasonic
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protocole
-
ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
Pureté et documentation
-
Fiche technique (277 KB)
-
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
-
Instruction de manipulation (2659 KB)
Références
[1]. Thangjam GS, et, al. Regulation of oxidative-stress responsive genes by arecoline in human keratinocytes. J Periodontal Res. 2009 Oct;44(5):673-82. [Content Brief]
[2]. Liu YJ, et, al. The pharmacology, toxicology and potential applications of arecoline: a review. Pharm Biol. 2016 Nov;54(11):2753-2760. [Content Brief]
[3]. Volgin AD, et, al. DARK Classics in Chemical Neuroscience: Arecoline. ACS Chem Neurosci. 2019 May 15;10(5):2176-2185. [Content Brief]
[4]. Kuo TM, et, al. Arecoline induces TNF-alpha production and Zonula Occludens-1 redistribution in mouse Sertoli TM4 cells. J Biomed Sci. 2014 Sep 9;21(1):93. [Content Brief]
[5]. Chen QY, et al. Inhibition of cortical synaptic transmission, behavioral nociceptive, and anxiodepressive-like responses by arecoline in adult mice. Mol Brain. 2024 Jun 17;17(1):39. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 4.2353 mL | 21.1766 mL | 42.3531 mL | 105.8829 mL |
| 5 mM | 0.8471 mL | 4.2353 mL | 8.4706 mL | 21.1766 mL | |
| 10 mM | 0.4235 mL | 2.1177 mL | 4.2353 mL | 10.5883 mL | |
| 15 mM | 0.2824 mL | 1.4118 mL | 2.8235 mL | 7.0589 mL | |
| 20 mM | 0.2118 mL | 1.0588 mL | 2.1177 mL | 5.2941 mL | |
| 25 mM | 0.1694 mL | 0.8471 mL | 1.6941 mL | 4.2353 mL | |
| 30 mM | 0.1412 mL | 0.7059 mL | 1.4118 mL | 3.5294 mL | |
| 40 mM | 0.1059 mL | 0.5294 mL | 1.0588 mL | 2.6471 mL | |
| 50 mM | 0.0847 mL | 0.4235 mL | 0.8471 mL | 2.1177 mL | |
| 60 mM | 0.0706 mL | 0.3529 mL | 0.7059 mL | 1.7647 mL | |
| 80 mM | 0.0529 mL | 0.2647 mL | 0.5294 mL | 1.3235 mL | |
| 100 mM | 0.0424 mL | 0.2118 mL | 0.4235 mL | 1.0588 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Arecoline hydrobromide Related Classifications
HY-B0489 Related Classifications
Powered by Bioz