KH7
Based on 7 publication(s) in Google Scholar
KH7 is a soluble adenylyl cyclase (sAC)-specific inhibitor, with IC50s of 3-10 μM toward both recombinant purified human sACt protein and heterologously expressed sACt in cellular assays. KH7 is also a cAMP inhibitor.
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- Pureté: 99.0%
- CAS No.: 330676-02-3
- Formule: C17H15BrN4O2S
- Masse moléculaire:419.30
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Stockage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) KH7
More- Mol Cell. 2020 Apr 2;78(1):42-56.e6. [Abstract]
- Cell Rep. 2021 Sep 21;36(12):109726. [Abstract]
- J Agric Food Chem. 2025 Aug 13;73(32):20235-20253. [Abstract]
- J Cell Mol Med. 2020 Apr;24(8):4736-4747. [Abstract]
- iScience. 2025 Jan 19;28(2):111833. [Abstract]
- J Cell Physiol. 2020 Dec;235(12):9510-9523. [Abstract]
- J Integr Neurosci. 2025 Aug 26;24(8):42286. [Abstract]
Customer Validation & Images
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WB
Activité biologique
IC50: 3-10 μM (recombinant sACt)[1].
KH7 (10 μM) blocks this capacitation-induced cAMP increase. At higher concentrations (50 μM), 5- to 10-fold above its IC50 but still selective for sAC relative to tmACs, KH7 resulted in a significant decrease in the basal cAMP accumulation in sperm regardless of the incubation medium[1].
KH7 prevents the generation of CaSF[2].
In the presence of KH7, the myocytes exerts a negative inotropic effect (NIE) of approximately 20%, suggesting that sAC is involved in the normal generation of basal cardiac contractility[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 330676-02-3
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Appearance Solid
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Masse moléculaire 419.30
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Formule C17H15BrN4O2S
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Color White to light yellow
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SMILES
CC(SC1=NC2=CC=CC=C2N1)C(N/N=C/C3=CC(Br)=CC=C3O)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (7)
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Journal Impact Factor
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Most Recent
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Mol Cell
Dopamine Uses the DRD5-ARRB2-PP2A Signaling Axis to Block the TRAF6-Mediated NF-κB Pathway and Suppress Systemic Inflammation. [Abstract]2020 Apr 2;78(1):42-56.e6. PMID: 32035036 -
Cell Rep
Gut microbiota-mediated secondary bile acids regulate dendritic cells to attenuate autoimmune uveitis through TGR5 signaling. [Abstract]2021 Sep 21;36(12):109726. PMID: 34551302 -
J Agric Food Chem
Sargassum fusiforme Fucoidan Ameliorates Obesity-Associated Metabolic Dysfunction via a Tauroursodeoxycholic Acid-Mediated TGR5-cAMP-PKA Signaling Pathway. [Abstract]2025 Aug 13;73(32):20235-20253. PMID: 40758868 -
J Cell Mol Med
Maresin Conjugates in Tissue Regeneration 1 improves alveolar fluid clearance by up-regulating alveolar ENaC, Na, K-ATPase in lipopolysaccharide-induced acute lung injury. [Abstract]2020 Apr;24(8):4736-4747. PMID: 32160403 -
iScience
2025 Jan 19;28(2):111833. PMID: 39949963 -
J Cell Physiol
PCTR1 improves pulmonary edema fluid clearance through activating the sodium channel and lymphatic drainage in lipopolysaccharide-induced ARDS. [Abstract]2020 Dec;235(12):9510-9523. PMID: 32529661
KH7 purchased from MedChemExpress. Usage Cited in: J Cell Physiol. 2020 Dec;235(12):9510-9523. [Abstract]
The P‐Akt protein expression is measured by Western blot analysis. The improved effects of PCTR1 on P‐AKT are partially blocked by KH7.
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J Integr Neurosci
A Low Sodium Diet Activates HSD2 Neurons in the Nucleus Tractus Solitarii to Promote Sodium Appetite Via the cAMP/MAPK Signaling Pathway. [Abstract]2025 Aug 26;24(8):42286. PMID: 40919635
Solvant et solubilité
DMSO : 250 mg/mL (596.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (5.96 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.96 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (273 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Hess KC, et al. The "soluble" adenylyl cyclase in sperm mediates multiple signaling events required for fertilization. Dev Cell. 2005 Aug;9(2):249-59. [Content Brief]
[2]. Han J, et al. Maresin Conjugates in Tissue Regeneration 1 improves alveolar fluid clearance by up-regulating alveolar ENaC, Na, K-ATPase in lipopolysaccharide-induced acute lung injury. 4.658J Cell Mol Med. 2020 Mar 11. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3849 mL | 11.9246 mL | 23.8493 mL | 59.6232 mL |
| 5 mM | 0.4770 mL | 2.3849 mL | 4.7699 mL | 11.9246 mL | |
| 10 mM | 0.2385 mL | 1.1925 mL | 2.3849 mL | 5.9623 mL | |
| 15 mM | 0.1590 mL | 0.7950 mL | 1.5900 mL | 3.9749 mL | |
| 20 mM | 0.1192 mL | 0.5962 mL | 1.1925 mL | 2.9812 mL | |
| 25 mM | 0.0954 mL | 0.4770 mL | 0.9540 mL | 2.3849 mL | |
| 30 mM | 0.0795 mL | 0.3975 mL | 0.7950 mL | 1.9874 mL | |
| 40 mM | 0.0596 mL | 0.2981 mL | 0.5962 mL | 1.4906 mL | |
| 50 mM | 0.0477 mL | 0.2385 mL | 0.4770 mL | 1.1925 mL | |
| 60 mM | 0.0397 mL | 0.1987 mL | 0.3975 mL | 0.9937 mL | |
| 80 mM | 0.0298 mL | 0.1491 mL | 0.2981 mL | 0.7453 mL | |
| 100 mM | 0.0238 mL | 0.1192 mL | 0.2385 mL | 0.5962 mL |
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