Rhod-2 AM

Rhod-2 AM purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Jan 10:e2415030. [Abstract]

Customer Validation
CLSM images showing membrane integrity and calcium levels of mitochondria in 4T1 cells treated with various nanoreactors. Rhod‐2 AM was dissolved in DMSO containing 0.02% Pluronic F127 and diluted using PBS to a final concentration of 5 µM. 4T1 cells were seeded in confocal dishes and cultured for 24 h. Nanoreactors (200 µg/mL) were added to the culture for an additional 24 h. Following three washes with PBS, the cells were incubated with the probe for 30 min, followed by imaging with CLSM.

Rhod-2 AM purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 24;16(1):6821. [Abstract]

Customer Validation
To detect the level of Ca²⁺ in mitochondria, C. albicans cells were stained with Rhod-2 AM (5 μM, 30 min) at 37 °C. The images were acquired utilizing a confocal microscope. The excitation and emission wavelengths for the fluorescent probes used in this study are as follows: ER-Tracker Red (587 nm/615 nm), Rhod-2AM (549 nm/578 nm).

Rhod-2 AM purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Nov 21;11(47):eadz4126. [Abstract]

Customer Validation
RAW 264.7 cells were harvested and stained with a mitochondrial Ca²⁺ indicator (Rhod-2 AM) at room temperature for 30 min.

Rhod-2 AM purchased from MedChemExpress. Usage Cited in: J Adv Res. 2024 May 7:S2090-1232(24)00175-9. [Abstract]

Customer Validation
To detect intracellular Ca²⁺, cells were treated with 1 μM of Rhod-2 AM fluorescent probes and subsequently washed. The intracellular Ca²⁺ levels were quantified.

Rhod-2 AM purchased from MedChemExpress. Usage Cited in: Environ Sci Technol. 2017 Dec 5;51(23):13938-13948. [Abstract]

Customer Validation
Cellular staining with Fluo-3/AM, Rhod-2 and DAPI (blue) in cells upon nLa₂O₃ treatment at 20 μg/mL for 6 h. Cells are examined with confocal microscopy. Two colors are showed individually or merged together.