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Acridine Orange base

43 Cited Publications
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Based on 43 publication(s) in Google Scholar

Acridine Orange base is a cell-permeable fluorescent dye that stains organisms (bacteria, parasites, viruses, etc.) bright orange and, when used under appropriate conditions (pH=3.5, Ex=460 nm), distinguishes human cells in green for detection by fluorescence microscopy. Acridine Orange base fluoresces green when bound to dsDNA (Ex=488, Em=520-524) and red when bound to ssDNA (Ex=457, Em=630-644) or ssRNA (Ex=457, Em=630-644), also can be used in cell cycle assays.

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  • Pureté: 96.58%
  • CAS No.: 494-38-2
  • Formule: C17H19N3
  • Masse moléculaire:265.36
  • Stockage:

    4°C, protect from light

    * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

  • Acridine Orange base Chemical Structure
    Acridine Orange base Chemical Structure
  • Experimental Validation Results for Acridine Orange base
  • Experimental Validation Results for Acridine Orange base
  • Experimental Validation Results for Acridine Orange base
  • Experimental Validation Results for Acridine Orange base
  • Experimental Validation Results for Acridine Orange base
  • Experimental Validation Results for Acridine Orange base
  • Experimental Validation Results for Acridine Orange base
CAS No.: 494-38-2
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  • Acridine Orange base Chemical Structure
    Acridine Orange base Chemical Structure
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    Acridine Orange base purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2025 Mar;22(3):282-299. [Abstract]

    Customer Validation
     Human PBMCs (H-PBMCs) and Jurkat cells were treated with OA (5, 50, or 500 μM) or 200 ng/mL PMA for 24 hours, after which the cells were stained with acridine orange (AO).

  • 1/16

    Acridine Orange base purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Jun 30;14(1):3877. [Abstract]

    Customer Validation
     Acridine Orange (AO, 5 μM) was added immediately after the ultrasound treatment and was incubated for 30 min at 37°C before confocal microscopy.

  • 1/16

    Acridine Orange base purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2021 Oct;11(10):3178-3192. [Abstract]

    Customer Validation
     The Jurkat cells were treated with LW-218 (8 μmol/L) for 0, 1, 2, and 4 h. Before collecting, the cells were stained with 5 μmol/L Acridine Orange hydrochloride solution for 15 min.

  • 1/16

    Acridine Orange base purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2021 Jan 13;12(1):80. [Abstract]

    Customer Validation
     The Jurkat cells treated with 25 μM FV-429 for 12 h were stained with Acridine Orange hydrochloride (AO, 5 μM; 15 min) before collection, and the AO fluorescence was determined by a laser confocal microscope. Scale bar: 10 μm.

  • 1/16

    Acridine Orange base purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2021 Jan 13;12(1):80. [Abstract]

    Customer Validation
     The Jurkat and Hut102 cells treated with 25 μM FV-429 for 12 h were stained with Acridine Orange hydrochloride (AO, 5 μM; 15 min) staining before collecting, and the AO fluorescences were determined by laser confocal microscope. Scale bar: 5 μm.

  • 1/16

    Acridine Orange base purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2022 Jan;55(1):e13172. [Abstract]

    Customer Validation
     CLSM images of acridine orange (AO)-stained osteoclasts cultured on PDMS substrates were taken; the cells were stained with 1 μM AO at 37°C for 20 min.

  • 1/16

    Acridine Orange base purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Jul 30;11(7):607. [Abstract]

    Customer Validation
     Depending on the acidity, Acridine Orange hydrochloride (AO) causes autophagic lysosomes to appear as orange/red fluorescent vesicles, while it caused nuclei to appear green. AO staining demonstrates that LA dose-dependently increased the numbers of intracellular acidic compartments.

  • Acridine Orange base