[BMIM]Cl
Based on 1 Customer Validation
[BMIM]Cl (1-butyl-3-methylimidazolium chloride) is an alkyl-imidazolium chloride compound and a persistent aquatic pollutant. [BMIM]Cl exhibits antimicrobial activity against S. aureus (MIC = 25 mM), E. coli (MIC = 50 mM), and P. aeruginosa (MIC = 100 mM). It possesses moderate membrane permeability and cytotoxicity, directly affecting microorganisms and mammalian cells at high concentrations, but can inhibit the function of complex ecosystems (e.g., anaerobic digestion) even at environmentally relevant low concentrations by disrupting microbial community structures. [BMIM]Cl can be used in research related to bacterial infections.
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- Pureté: 99.91%
- CAS No.: 79917-90-1
- Formule: C8H15ClN2
- Masse moléculaire:174.67
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Stockage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
![[BMIM]Cl Chemical Structure](https://file.medchemexpress.com/product_pic/hy-w016758.gif "Click to View [BMIM]Cl Chemical Structure")
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Caco-2 | IC50 |
18.85 mM
Compound: [BMIM][Cl]
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Cytotoxicity against human Caco2 cells after 24 hrs by MTT assay
Cytotoxicity against human Caco2 cells after 24 hrs by MTT assay
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[PMID: 26617961] |
[BMIM]Cl (0-200 mM; 18 hours) inhibits the growth of E. coli with an MIC of 50 mM, S. aureus with an MIC of 25 mM, and P. aeruginosa with an MIC of 100 mM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 79917-90-1
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Appearance Solid
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Masse moléculaire 174.67
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Formule C8H15ClN2
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Color White to off-white
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SMILES
CN1C=C[N+](CCCC)=C1.[Cl-]
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Synonyms
1-Butyl-3-methylimidazolium chloride
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Solvant et solubilité
DMSO : ≥ 200 mg/mL (1145.02 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (28.63 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (28.63 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (271 KB)
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SDS (624 KB)
- English - EN (624 KB)
- Français - FR (624 KB)
- Deutsch - DE (624 KB)
- Norwegian - NO (624 KB)
- Español - ES (624 KB)
- Swedish - SV (624 KB)
- Italian - IT (624 KB)
- Korean - KR (624 KB)
- Portuguese - PT (624 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Cook K, et al. Correlating Lipid Membrane Permeabilities of Imidazolium Ionic Liquids with their Cytotoxicities on Yeast, Bacterial, and Mammalian Cells. Biomolecules. 2019;9(6):251. Published 2019 Jun 25. [Content Brief]
[2]. Lu Q, et al. 1-Butyl-3-methylimidazolium Chloride Affects Anaerobic Digestion through Altering Organics Transformation, Cell Viability, and Microbial Community. Environ Sci Technol. 2023;57(8):3145-3155. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.7251 mL | 28.6254 mL | 57.2508 mL | 143.1270 mL |
| 5 mM | 1.1450 mL | 5.7251 mL | 11.4502 mL | 28.6254 mL | |
| 10 mM | 0.5725 mL | 2.8625 mL | 5.7251 mL | 14.3127 mL | |
| 15 mM | 0.3817 mL | 1.9084 mL | 3.8167 mL | 9.5418 mL | |
| 20 mM | 0.2863 mL | 1.4313 mL | 2.8625 mL | 7.1564 mL | |
| 25 mM | 0.2290 mL | 1.1450 mL | 2.2900 mL | 5.7251 mL | |
| 30 mM | 0.1908 mL | 0.9542 mL | 1.9084 mL | 4.7709 mL | |
| 40 mM | 0.1431 mL | 0.7156 mL | 1.4313 mL | 3.5782 mL | |
| 50 mM | 0.1145 mL | 0.5725 mL | 1.1450 mL | 2.8625 mL | |
| 60 mM | 0.0954 mL | 0.4771 mL | 0.9542 mL | 2.3855 mL | |
| 80 mM | 0.0716 mL | 0.3578 mL | 0.7156 mL | 1.7891 mL | |
| 100 mM | 0.0573 mL | 0.2863 mL | 0.5725 mL | 1.4313 mL |
[BMIM]Cl Related Classifications
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