C16-VVVAAAEEE
C16-VVVAAAEEE is a peptide amphiphile and biodegradable nanofiber scaffold that self-assembles into a hydrogel upon calcium ion triggering, enabling delivery of Sonic hedgehog protein to corpus cavernosum smooth muscle with prolonged release and without eliciting a significant immune response. C16-VVVAAAEEE is used in research on erectile dysfunction.
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- CAS No.: 843663-78-5
- Formule: C55H95N9O17
- Masse moléculaire:1154.39
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
C16‑VVVAAAEEE PA gel (100 mM; 1‑141 h) releases SHH protein in a time‑dependent manner, with 73% diffusion observed within 141 h in vitro[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Sprague Dawley rats (postnatal day 115-120, bilateral cavernous nerve resection)[1]
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Dosage:20; 50; 100 µL (volume optimization); 50 µL (duration study); 10 mM final concentration
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Administration:Injection into corpora cavernosa; single administration
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Result:Visualized a highly brightened region of the penis at 20 µL.
Formed a gel uniformly throughout the entire penis at 50 µL.
Did not remain localized within the penis at 100 µL.
Remained localized completely in the penis when a silk tie was used.
Showed well dispersed fluorescence throughout both corpora cavernosa when injected distally.
Did not appear to reach all of the adjacent corpus cavernosum when injected proximally.
Formed a thin layer lining the sinusoidal spaces at 1 hour.
Was still abundant and clearly visible within the corpora cavernosa at 7 days.
Was observed as a nonspecific fluorescence indicating mostly degraded at 14 days.
Chemical Information
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CAS No. 843663-78-5
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Masse moléculaire 1154.39
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Formule C55H95N9O17
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Sequence
C16-Val-Val-Val-Ala-Ala-Ala-Glu-Glu-Glu
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Sequence Shortening
C16-VVVAAAEEE
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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3D Hydrogel Synthetic Scaffold Culture
3D hydrogel synthetic scaffold culture embeds cells, spheroids, organoids, or tissue fragments inside a hydrated crosslinked polymer network so that cells receive matrix and cell-cell cues in three dimensions rather than from a flat plastic surface. A literature-supported model protocol is PEG-4MAL hydrogel culture, in which four-arm maleimide-terminated PEG is functionalized with cysteine-containing adhesive peptides such as RGD and crosslinked with protease-degradable peptides such as GPQ-W; this creates a defined, modular scaffold that supports human organoid generation and culture. The readouts are scaffold-supported growth, morphology, lumen formation, budding, viability, proliferation, lineage-marker expression, and matrix-dependent expansion or differentiation; reported assays include transmitted-light imaging, immunofluorescence, in situ hybridization, qRT-PCR, and rheological characterization.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
C16-VVVAAAEEE Related Classifications
HY-P12356 Related Classifications
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Keywords
- C16-VVVAAAEEE
- 843663-78-5
- Biochemical Assay Reagents
- Sonic hedgehog protein
- extended protein release
- self-assembling peptide amphiphile nanofiber hydrogel
- erectile dysfunction
- hydrogel
- immune response
- calcium ions
- biodegradable nanofiber scaffold
- penile corpora cavernosal smooth muscle
- peptide amphiphile
- Inhibitor
- inhibitor
- inhibit