Heterophyllin B
Based on 5 publication(s) in Google Scholar
Heterophyllin B is an active cyclic peptide isolated from Pseudostellaria heterophylla. Heterophyllin B provides a novel strategy for the treatment of esophageal cancer.
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- Pureté : 99.93%
- CAS No.: 145459-19-4
- Formule: C40H58N8O8
- Masse moléculaire:778.94
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Stockage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Heterophyllin B
More
Customer Validation & Images
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Cell Proliferation/Viability Assay
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Apoptosis Analysis
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IF
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WB
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RT-PCR
Activité biologique
Description
In Vitro
Heterophyllin B (0-200 µM; 12-48 hours) reduces cell viability in the 75, 100 and 200 µM groups, has no effect on 5, 10, 25 and 50 µM groups[1]. Heterophyllin B (0-50 µM; 6 hours) suppresses the expression levels of p-PI3K, p-AKT and β-catenin in a dose-dependent manner in ECA-109 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:ECA-109 cells
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Concentration:5 µM, 10 µM, 25 µM, 50 µM, 75 µM, 100 µM and 200 µM
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Incubation Time:12, 24 and 48 hours
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Result:Decreased cell viability at high concentrations.
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Cell Line:ECA-109 cells
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Concentration:0 µM, 10 µM, 25 µM, 50 µM
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Incubation Time:6 hours
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Result:Decreased the phosphorylation of PI3K/AKT and the expression of β-catenin.
Chemical Information
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CAS No. 145459-19-4
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Appearance Solid
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Masse moléculaire 778.94
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Formule C40H58N8O8
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Color White to off-white
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SMILES
O=C([C@@](CCC1)([H])N1C([C@](NC(CNC(CNC2=O)=O)=O)([H])CC(C)C)=O)N(CCC3)[C@]3([H])C(N(CCC4)[C@]4([H])C(N[C@@](C(N[C@H]2CC5=CC=CC=C5)=O)([H])[C@@H](C)CC)=O)=O
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Structure Classification
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (5)
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Journal Impact Factor
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Most Recent
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Int J Biol Sci
Heterophyllin B enhances transcription factor EB-mediated autophagy and alleviates pyroptosis and oxidative stress after spinal cord injury. [Abstract]2024 Oct 7;20(14):5415-5435. PMID: 39494322 -
Sci Rep
HB reduces inflammation in HUVECs via the Nrf2/HO-1 axis by inhabiting apoptosis and oxidative stress. [Abstract]2026 Jun 23. PMID: 42336938
Heterophyllin B purchased from MedChemExpress. Usage Cited in: Sci Rep. 2026 Jun 23. [Abstract]
HUVECs were stimulated with different doses of Heterophyllin B (HB) (25, 50, 75, 100 μM) for 6 hours and cell viabiltiy analysis was performed using the CCK-8 assay.
Heterophyllin B purchased from MedChemExpress. Usage Cited in: Sci Rep. 2026 Jun 23. [Abstract]
Cells were pretreated with Heterophyllin B (HB) (25, 50, 100 μM) for 6 hours prior to H2O2 exposure. Following staining with Annexin V-FITC/PI, the apoptosis rate was detected by flow cytometry.
Heterophyllin B purchased from MedChemExpress. Usage Cited in: Sci Rep. 2026 Jun 23. [Abstract]
HUVECs were pretreated with various concentrations of Heterophyllin B (HB) (25, 50, 100 μM) and were stained with DCF.
Heterophyllin B purchased from MedChemExpress. Usage Cited in: Sci Rep. 2026 Jun 23. [Abstract]
Protein expression levels of TNF-α, IL-1β, and IL-6 in HUVECs exposed to 0, 25, 50, and 100 µM of Heterophyllin B (HB) for 6h prior to treatment with or without 100 µM H2O2 for 24h, as measured by western blot analysis.
Heterophyllin B purchased from MedChemExpress. Usage Cited in: Sci Rep. 2026 Jun 23. [Abstract]
mRNA expression of TNF-α, IL-1β, and IL-6 exposed to 0, 25, 50, and 100 µM of Heterophyllin B (HB) was examined by RT-PCR.
Heterophyllin B purchased from MedChemExpress. Usage Cited in: Sci Rep. 2026 Jun 23. [Abstract]
The contents of TNF-α, IL-1β, and IL-6 in the culture supernatants were measured by ELISA assay exposed to 0, 25, 50, and 100 µM of Heterophyllin B (HB).
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Tissue Cell
Anti-neoplastic effect of heterophyllin B on ovarian cancer via the regulation of NRF2/HO-1 in vitro and in vivo. [Abstract]2024 Dec:91:102566. PMID: 39341007 -
Tissue Cell
2023 Aug:83:102129. PMID: 37406539 -
Hum Cell
2023 Mar;36(2):676-688. PMID: 36539682
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (128.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.21 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.21 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2838 mL | 6.4190 mL | 12.8380 mL | 32.0949 mL |
| 5 mM | 0.2568 mL | 1.2838 mL | 2.5676 mL | 6.4190 mL | |
| 10 mM | 0.1284 mL | 0.6419 mL | 1.2838 mL | 3.2095 mL | |
| 15 mM | 0.0856 mL | 0.4279 mL | 0.8559 mL | 2.1397 mL | |
| 20 mM | 0.0642 mL | 0.3209 mL | 0.6419 mL | 1.6047 mL | |
| 25 mM | 0.0514 mL | 0.2568 mL | 0.5135 mL | 1.2838 mL | |
| 30 mM | 0.0428 mL | 0.2140 mL | 0.4279 mL | 1.0698 mL | |
| 40 mM | 0.0321 mL | 0.1605 mL | 0.3209 mL | 0.8024 mL | |
| 50 mM | 0.0257 mL | 0.1284 mL | 0.2568 mL | 0.6419 mL | |
| 60 mM | 0.0214 mL | 0.1070 mL | 0.2140 mL | 0.5349 mL | |
| 80 mM | 0.0160 mL | 0.0802 mL | 0.1605 mL | 0.4012 mL | |
| 100 mM | 0.0128 mL | 0.0642 mL | 0.1284 mL | 0.3209 mL |
Heterophyllin B Related Classifications
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