Mudanpioside C
Based on 1 Customer Validation
Mudanpioside C is a protein disulfide isomerase (PDI) inhibitor with a human IC50 of 3.31 μM and human Kd of 3.9 μM. Mudanpioside C suppresses collagen-induced platelet aggregation, interferes with platelet activation, adhesion, and spreading. Mudanpioside C can be used for the research of cardiovascular diseases.
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- Pureté : 98.20%
- CAS No.: 172760-03-1
- Formule: C30H32O13
- Masse moléculaire:600.57
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Stockage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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HY-N2499
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HY-100433
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HY-100433R
PACMA 31 (Standard)Protein Disulfide Isomerase Inhibitor -
HY-100432R
LOC14 (Standard)PDI Inhibitor -
HY-100430R
CCF642 (Standard)PDI Inhibitor
Activité biologique
Description
In Vitro
Mudanpioside C (140 min) potently inhibits purified recombinant PDI activity with an IC50 of 3.31 μM[1].
Mudanpioside C specifically binds to the b′-x domain of human PDI with a Kd of 3.9 μM, primarily interacting with amino acids K263, D292, and N298[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:ICR mice (15−20 g)[1]
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Dosage:0.6 mg/kg
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Administration:tail vein injection; single dose
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Result:Prolonged carotid artery occlusion time compared to the control group, with a statistically significant difference (P=0.0004).
Showed no significant difference in tail bleeding time compared to the control group.
Chemical Information
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CAS No. 172760-03-1
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Appearance Solid
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Masse moléculaire 600.57
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Formule C30H32O13
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Color White to off-white
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SMILES
O[C@H]([C@H]([C@@H]([C@@H](COC(C1=CC=C(O)C=C1)=O)O2)O)O)[C@@H]2O[C@@]3(C4)[C@@](C5)(C)O[C@]6([H])O[C@@]5(O)[C@@]4([H])[C@]36COC(C7=CC=CC=C7)=O
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvant et solubilité
In Vitro:
DMSO : 200 mg/mL (333.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (8.33 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (8.33 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocole
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Pureté et documentation
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Fiche technique (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Xie X, et al. Mudanpioside C Discovered from Paeonia suffruticosa Andr. Acts as a Protein Disulfide Isomerase Inhibitor with Antithrombotic Activities. J Agric Food Chem. 2024 Mar 27;72(12):6265-6275. [Content Brief]
[2]. Wang S, et al. A novel method HPLC-DAD analysis of the Contentsof Moutan Cortexand Paeoniae Radix Alba with similar constituents-monoterpene glycosides in Guizhi Fuling Wan. Molecules. 2014;19(11):17957-17967. Published 2014 Nov 4. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6651 mL | 8.3254 mL | 16.6508 mL | 41.6271 mL |
| 5 mM | 0.3330 mL | 1.6651 mL | 3.3302 mL | 8.3254 mL | |
| 10 mM | 0.1665 mL | 0.8325 mL | 1.6651 mL | 4.1627 mL | |
| 15 mM | 0.1110 mL | 0.5550 mL | 1.1101 mL | 2.7751 mL | |
| 20 mM | 0.0833 mL | 0.4163 mL | 0.8325 mL | 2.0814 mL | |
| 25 mM | 0.0666 mL | 0.3330 mL | 0.6660 mL | 1.6651 mL | |
| 30 mM | 0.0555 mL | 0.2775 mL | 0.5550 mL | 1.3876 mL | |
| 40 mM | 0.0416 mL | 0.2081 mL | 0.4163 mL | 1.0407 mL | |
| 50 mM | 0.0333 mL | 0.1665 mL | 0.3330 mL | 0.8325 mL | |
| 60 mM | 0.0278 mL | 0.1388 mL | 0.2775 mL | 0.6938 mL | |
| 80 mM | 0.0208 mL | 0.1041 mL | 0.2081 mL | 0.5203 mL | |
| 100 mM | 0.0167 mL | 0.0833 mL | 0.1665 mL | 0.4163 mL |
Mudanpioside C Related Classifications
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