RO7185876
Based on 1 Customer Validation
RO7185876 is a potent and selective gamma secretase modulator as a potential research for Alzheimer's disease.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté: 99.9%
- CAS No.: 2226038-71-5
- Formule: C25H28F3N7
- Masse moléculaire:483.53
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | IC50 |
2 nM
Compound: (S)-3; RO7185876
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Inhibition of human Notch1 expressed in HEK293 cells coexpressing luciferase reporter measured after 20 hrs by chemiluminescence assay
Inhibition of human Notch1 expressed in HEK293 cells coexpressing luciferase reporter measured after 20 hrs by chemiluminescence assay
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[PMID: 32551009] |
| HEK293 | IC50 |
2 nM
Compound: (S)-3; RO7185876
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Modulation of human gamma secretase expressed in HEK293 cells assessed as reduction in total amyloid beta (1 to 42 residues) production
Modulation of human gamma secretase expressed in HEK293 cells assessed as reduction in total amyloid beta (1 to 42 residues) production
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[PMID: 32551009] |
| HEK293 | IC50 |
4 nM
Compound: (S)-3; RO7185876
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Modulation of human gamma secretase expressed in HEK293 cells assessed as reduction in free amyloid beta (1 to 42 residues) production
Modulation of human gamma secretase expressed in HEK293 cells assessed as reduction in free amyloid beta (1 to 42 residues) production
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[PMID: 32551009] |
| N2a | IC50 |
2 nM
Compound: (S)-3; RO7185876
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Modulation of mouse gamma secretase in N2A cells assessed as reduction in total amyloid beta (1 to 42 residues) production
Modulation of mouse gamma secretase in N2A cells assessed as reduction in total amyloid beta (1 to 42 residues) production
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[PMID: 32551009] |
| N2a | IC50 |
4 nM
Compound: (S)-3; RO7185876
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Modulation of mouse gamma secretase in N2A cells assessed as reduction in free amyloid beta (1 to 42 residues) production
Modulation of mouse gamma secretase in N2A cells assessed as reduction in free amyloid beta (1 to 42 residues) production
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[PMID: 32551009] |
Chemical Information
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CAS No. 2226038-71-5
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Appearance Solid
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Masse moléculaire 483.53
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Formule C25H28F3N7
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Color White to off-white
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SMILES
FC(C(F)=C(C=C1)F)=C1[C@@]2([H])C3=NC(N[C@H]4[C@@H]5CN(C6=NC=NC(C)=C6)C[C@H]4CC5)=NN3CCCC2
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
DMSO : 100 mg/mL (206.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.17 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.17 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (277 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0681 mL | 10.3406 mL | 20.6812 mL | 51.7031 mL |
| 5 mM | 0.4136 mL | 2.0681 mL | 4.1362 mL | 10.3406 mL | |
| 10 mM | 0.2068 mL | 1.0341 mL | 2.0681 mL | 5.1703 mL | |
| 15 mM | 0.1379 mL | 0.6894 mL | 1.3787 mL | 3.4469 mL | |
| 20 mM | 0.1034 mL | 0.5170 mL | 1.0341 mL | 2.5852 mL | |
| 25 mM | 0.0827 mL | 0.4136 mL | 0.8272 mL | 2.0681 mL | |
| 30 mM | 0.0689 mL | 0.3447 mL | 0.6894 mL | 1.7234 mL | |
| 40 mM | 0.0517 mL | 0.2585 mL | 0.5170 mL | 1.2926 mL | |
| 50 mM | 0.0414 mL | 0.2068 mL | 0.4136 mL | 1.0341 mL | |
| 60 mM | 0.0345 mL | 0.1723 mL | 0.3447 mL | 0.8617 mL | |
| 80 mM | 0.0259 mL | 0.1293 mL | 0.2585 mL | 0.6463 mL | |
| 100 mM | 0.0207 mL | 0.1034 mL | 0.2068 mL | 0.5170 mL |
RO7185876 Related Classifications
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