2. Cell Cycle/DNA Damage Apoptosis
  3. RAD51 Apoptosis
  4. CAM833

CAM833 is a potent orthosteric inhibitor of the interaction between BRCA2 and RAD51 with a Kd of 366 nM against the ChimRAD51 protein. CAM833 also inhibits RAD51 oligomerization. CAM833 increases the progression of G2/M-arrested cells into apoptosis.

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CAM833 Chemical Structure

CAM833 Chemical Structure

CAS No. : 2758364-02-0

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 465 In-stock
Solution
10 mM * 1 mL in DMSO USD 465 In-stock
Solid
5 mg USD 400 In-stock
10 mg USD 680 In-stock
25 mg USD 1380 In-stock
50 mg USD 2200 In-stock
100 mg USD 3580 In-stock
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Based on 1 publication(s) in Google Scholar

CAM833 Related Antibodies

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Description

CAM833 is a potent orthosteric inhibitor of the interaction between BRCA2 and RAD51 with a Kd of 366 nM against the ChimRAD51 protein. CAM833 also inhibits RAD51 oligomerization. CAM833 increases the progression of G2/M-arrested cells into apoptosis[1].

IC50 & Target

Kd: 355 nM (ChimRAD51, measured by FP), 366 nM (ChimRAD51, measured by ITC)[1]
In Vitro

CAM833 (3.125-50 μM; 24 h) causes a concentration-dependent decrease in RAD51 foci and subsequent increase in DNA damage[1].
CAM833 (25 μM) inhibits RAD51 molecular clustering at DNA damage sites and suppresses extended RAD51 filament assembly[1].
CAM833 (0-50 μM) inhibits DNA repair by homologous recombination[1].
CAM833 (20 μM; 0-72 h) potentiates radiation-induced cell-cycle arrest and increases apoptosis over time in HCT116 cells[1].
CAM833 (0.1-100 μM; 96 h) causes a dose-dependent growth inhibition of multiple cancer-derived human cell lines that is enhanced when combined with ionizing radiation[1].
CAM833 (20 μM; 96 h) potentiates the growth suppressive effect of PARP1 inhibition in BRCA2 wild-type cells[1].
CAM833 (96 h) alone inhibits the growth of HCT116 colon carcinoma cells with a GI50 (50% growth inhibition) of 38 μM, when combined with 3 Gy IR, CAM833 suppresses the growth of HCT116 cells with a GI50 of 14 μM[1].
The quinoline of CAM833 occupies a hotspot, the Phe-binding pocket on RAD51 and the methyl of the substituted α-methylbenzyl group occupies the Ala-binding pocket[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CAM833 Related Antibodies

Cell Cycle Analysis[1]

Cell Line: HCT116 cells
Concentration: 20 μM
Incubation Time: 0-72 h
Result: In the control the percentage of cells in the apoptotic subG1 fraction remains below 5% throughout, in the compound-treated cells this rises progressively to peak at 15% at 48 hours.
Molecular Weight

528.96

Formula

C26H26ClFN4O5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@H](C1=C(C=C(C=C1)OC)Cl)NC([C@H]2N(C[C@@H](C2)O)C(CNC(C3=NC4=C(C=C(C=C4)F)C=C3)=O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (236.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8905 mL 9.4525 mL 18.9050 mL
5 mM 0.3781 mL 1.8905 mL 3.7810 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.79%

COA (255 KB) HNMR (330 KB) LCMS (266 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8905 mL 9.4525 mL 18.9050 mL 47.2626 mL
5 mM 0.3781 mL 1.8905 mL 3.7810 mL 9.4525 mL
10 mM 0.1891 mL 0.9453 mL 1.8905 mL 4.7263 mL
15 mM 0.1260 mL 0.6302 mL 1.2603 mL 3.1508 mL
20 mM 0.0945 mL 0.4726 mL 0.9453 mL 2.3631 mL
25 mM 0.0756 mL 0.3781 mL 0.7562 mL 1.8905 mL
30 mM 0.0630 mL 0.3151 mL 0.6302 mL 1.5754 mL
40 mM 0.0473 mL 0.2363 mL 0.4726 mL 1.1816 mL
50 mM 0.0378 mL 0.1891 mL 0.3781 mL 0.9453 mL
60 mM 0.0315 mL 0.1575 mL 0.3151 mL 0.7877 mL
80 mM 0.0236 mL 0.1182 mL 0.2363 mL 0.5908 mL
100 mM 0.0189 mL 0.0945 mL 0.1891 mL 0.4726 mL
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CAM833 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CAM8332758364-02-0CAM 833CAM-833RAD51ApoptosisBRCA2DNA damagehomologous recombinationDNA repaircancer therapyInhibitorinhibitorinhibit

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