CB1151
CB1151 is a 20-epi analogue of 1,25 dihydroxyvitamin D3 (VD) with potent anti-tumor effects. CB1151 inhibits MCF-7 cell growth with an IC50 value of 0.82 nM.
For research use only. We do not sell to patients.
- CAS No.: 182369-28-4
- Formula: C28H46O4
- Molecular Weight:446.66
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
IC50: 0.82 nM[1]
CB1151 (0-100 nM; 5 days) inhibits MCF-7 cells growth, this cell proliferation is accessed by [3H]-thymidine incorporation, exhibits an IC50 value of 0.82 nM[1]. CB1151 (0-100 nM; 40 hours) exhibits activation of the IP9-type VD response element with a EC50 of 1.2 nM, the activation of IP9-type VD response elements shows a good correlation with inhibition of proliferation than the activation of DR3-type elements (EC50=3.2nM) in MCF-7 cells transfected with the CAT reporter[1]. CB1151 shows a functional dissociation constant (Kdf) value of 3.6 nM. The ligand concentration that provides 50% of protease-resistant VDR fragment is defined by Kdf, this is different from that of the traditional dissociation constant (Kd)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7 cell
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Concentration:0 nM; 0.1nM; 0.5nM; 0.8nM; 1nM; 5nM; 10 nM
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Incubation Time:5 days
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Result:Inhibits MCF-7 cells growth.
Chemical Information
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CAS No. 182369-28-4
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Appearance Solid
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Molecular Weight 446.66
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Formula C28H46O4
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Color White to off-white
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SMILES
C[C@]1(CCC/2)[C@](CC[C@@H]1[C@@H](C)OCCC(CC)(CC)O)([H])C2=C\C=C(C[C@@H](O)C[C@@H]3O)/C3=C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 100 mg/mL (223.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Mørk Hansen C, et al. The potent anti-proliferative effect of 20-epi analogues of 1,25 dihydroxyvitamin D3 in human breast-cancer MCF-7 cells is related to promoter selectivity.Int J Cancer. 1996 Sep 4;67(5):739-42. [Content Brief]
[2]. Nayeri S, et al. High-affinity nuclear receptor binding of 20-epi analogues of 1,25-dihydroxyvitamin D3 correlates well with gene activation.J Cell Biochem. 1996 Sep 1;62(3):325-33. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2388 mL | 11.1942 mL | 22.3884 mL | 55.9710 mL |
| 5 mM | 0.4478 mL | 2.2388 mL | 4.4777 mL | 11.1942 mL | |
| 10 mM | 0.2239 mL | 1.1194 mL | 2.2388 mL | 5.5971 mL | |
| 15 mM | 0.1493 mL | 0.7463 mL | 1.4926 mL | 3.7314 mL | |
| 20 mM | 0.1119 mL | 0.5597 mL | 1.1194 mL | 2.7985 mL | |
| 25 mM | 0.0896 mL | 0.4478 mL | 0.8955 mL | 2.2388 mL | |
| 30 mM | 0.0746 mL | 0.3731 mL | 0.7463 mL | 1.8657 mL | |
| 40 mM | 0.0560 mL | 0.2799 mL | 0.5597 mL | 1.3993 mL | |
| 50 mM | 0.0448 mL | 0.2239 mL | 0.4478 mL | 1.1194 mL | |
| 60 mM | 0.0373 mL | 0.1866 mL | 0.3731 mL | 0.9328 mL | |
| 80 mM | 0.0280 mL | 0.1399 mL | 0.2799 mL | 0.6996 mL | |
| 100 mM | 0.0224 mL | 0.1119 mL | 0.2239 mL | 0.5597 mL |
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