2. Others
  3. Fluorescent Dye
  4. CFSE-Tz

CFSE-Tz is a fluorescent bioorthogonal probe constructed based on the IEDDA reaction, which is mainly used for visual tracing of amino acid uptake in living cells. The tetrazine group within the CFSE-Tz molecule quenches CFSE fluorescence via photoinduced electron transfer; after the probe specifically undergoes a bioorthogonal reaction with trans-cyclooctene-modified amino acids, the quenching effect is eliminated, and a green fluorescent signal is released, thereby dynamically monitoring the intracellular amino acid uptake process. CFSE-Tz can be applied to in vitro single-cell fluorescence imaging and studies related to amino acid metabolism in tumor cells (Ex = 495 nm, Em = 519 nm)[1][2].

For research use only. We do not sell to patients.

CFSE-Tz

CFSE-Tz Chemical Structure

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

CFSE-Tz Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

CFSE-Tz is a fluorescent bioorthogonal probe constructed based on the IEDDA reaction, which is mainly used for visual tracing of amino acid uptake in living cells. The tetrazine group within the CFSE-Tz molecule quenches CFSE fluorescence via photoinduced electron transfer; after the probe specifically undergoes a bioorthogonal reaction with trans-cyclooctene-modified amino acids, the quenching effect is eliminated, and a green fluorescent signal is released, thereby dynamically monitoring the intracellular amino acid uptake process. CFSE-Tz can be applied to in vitro single-cell fluorescence imaging and studies related to amino acid metabolism in tumor cells (Ex = 495 nm, Em = 519 nm)[1][2].

In Vitro

CFSE-Tz (compound 1) (1 μM; 1 hour, up to 80 minutes) and its intracellular product mimic CFSE-Tz-amide (compound 17) exhibit a dose-independent fluorescence turn-on effect after esterase hydrolysis and an IEDDA reaction with strained alkene-modified nutrient analogs; among these, the nutrient modified with trans-cyclooctene shows the fastest reaction rate[1].
CFSE-Tz exhibits no toxicity toward HeLa cells[1].
CFSE-Tz (0-50 μM; 30 minutes, 15-120 minutes) enables concentration- and time-dependent detection of energy-dependent nutrient uptake in HeLa cells[1].
CFSE-Tz (50 μM; 30 minutes, up to 80 minutes) enables real-time live-cell monitoring of nutrient uptake in HeLa cells, and the intracellular IEDDA reaction achieves a completion rate of >80% within 15 minutes of nutrient uptake[1].
CFSE-Tz (50 μM; 30 minutes) enables live-cell, single-cell visualization of nutrient uptake in HeLa cells via confocal microscopy, with the fluorescent signal increasing over time as nutrient uptake and intracellular IEDDA reactions occur[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CFSE-Tz Related Antibodies
Molecular Weight

739.65

Formula

C40H29N5O11
SMILES

O=C1OC2(C(C=CC(OC(C)=O)=C3)=C3OC4=C2C=CC(OC(C)=O)=C4)C5=CC=CC=C51.O=C(ON6C(CCC6=O)=O)C(C=C7)=CC=C7C(N=N8)=NN=C8/C=C/C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

CFSE-Tz Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CFSE-TzFluorescent Dyeconfocal microscopyHeLa cellsflow cytometryinverse electron-demand Diels-Alder reactionglutamatestrained alkene-modified nutrient analoguesASCT2cytosolic esteraseplate-reader assaysglycansInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
CFSE-Tz
Cat. No.:
HY-D3431
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.