CGN peptide

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing gene transcription or activating RNA degradation. This mechanism was discovered in plants in 1998 by Andrew Fire and Craig Mello. Today, this phenomenon can be observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals.

Cerebellar granule neuron culture is a primary neuronal culture method in which postnatal rodent cerebella are dissected, meninges are removed, tissue is enzymatically and mechanically dissociated, and neurons are plated on poly-cation-coated surfaces in medium that supports neuronal attachment, maturation, neurite-network formation, and biochemical or imaging analysis. Cultured CGNs are used to study neuronal development, survival, apoptosis, differentiation, neurotransmitter release, and toxin-sensitive synaptic proteins; mature cultures develop dense neuritic networks, neuronal activity, glutamate release, and neuronal marker expression. A common survival paradigm uses depolarizing extracellular potassium: CGNs maintained in high potassium medium survive and differentiate, whereas switching mature cultures from 25 mM KCl to 5 mM KCl induces apoptotic death that can be used as a readout of activity-dependent neuronal survival.

Alzheimer’s Disease (AD) is a neurodegenerative disorder characterized by a progressive decline in cognitive functions and loss of specific types of neurons and synapses. Alzheimer's symptoms can be simulated in mice by injecting drugs (such as Aβ) or genetically modified.