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Art. -Nr.: HY-167201

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2ccPA

Name: 2ccPA
Brand: MedChemExpress
SKU: HY-167201
Availability: PreOrder

(Synonyms: 2ccPA18:1)

2ccPA (2ccPA18:1) is a metabolically stabilized carba analog of cyclic phosphatidic acid (cPA). 2ccPA inhibits autotaxin (ATX)‑mediated lysophosphatidic acid (LPA) production, suppresses extracellular matrix biosynthesis and elevates intracellular cAMP in fibrogenic skin fibroblasts. 2ccPA protects oligodendrocytes from mitochondrial‑driven apoptosis, suppresses neuroinflammation and demyelination, modulates microglial polarization and promotes rapid‑platelet/fibrin‑dependent hemostasis and subsequent fibrinolytic transition following brain injury.

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CAS. Nr.: 633278-53-2
Formel: C₂₂H₄₁O₅P
Molecular Weight:416.53

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (277 KB)

2ccPA Chemical Structure

CAS. Nr.: 633278-53-2

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

Collagen I

In Vitro

2ccPA (1-10 μM; 48-72 h) does not reduce the viability of SSc skin fibroblasts or healthy skin fibroblasts, while 2ccPA (100 μM; 48-72 h) significantly reduces viability of SSc skin fibroblasts at these time points^[1].
2ccPA (10 μM; 48 h) inhibits the upregulation of profibrotic mRNA markers (COL1A1, COL1A2, CTGF, ACTA2) in TGF-β₁-stimulated healthy skin fibroblasts^[1].
2ccPA (10 μM; 72 h) reduces the protein expression of profibrotic markers (type I collagen, CCN2, αSMA) in TGF-β1-stimulated healthy skin fibroblasts^[1].
2ccPA (1-10 μM; 48 h) dose-dependently reduces profibrotic mRNA marker (COL1A1, COL1A2, CTGF, ACTA2) expression in SSc skin fibroblasts, and 2ccPA (10 μM; 6-48 h) maintains this inhibitory effect on additional markers (FN, TGF-β₁) for up to 48 h^[1].
2ccPA (10 μM; 48 h) reduces the protein expression of type I collagen and CCN2 in SSc skin fibroblasts, but 2ccPA (10 μM; 72 h) does not significantly decrease procollagen type I levels in culture supernatant^[1].
2ccPA (10 μM; 24 h) significantly elevates the production of antifibrotic factors HGF and PGE₂ in SSc skin fibroblasts^[1].
2ccPA (1-10 μM; 30 min) dose-dependently increases intracellular cAMP levels in SSc skin fibroblasts^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

systemic sclerosis (SSc) skin fibroblasts, healthy skin fibroblasts
Concentration:

1, 10, 100 μM
Incubation Time:

48 h, 72 h
Result:

Did not affect the viability of SSc skin fibroblasts and healthy skin fibroblasts at 1-10 μM for 48 or 72 h.
Significantly reduced SSc skin fibroblast viability at 100 μM for 48 and 72 h.
Showed a similar viability profile in healthy skin fibroblasts.

Real Time qPCR^[1]

Cell Line:

healthy skin fibroblasts treated with TGF-β₁
Concentration:

10 μM
Incubation Time:

48 h
Result:

Downregulated the mRNA expression levels of extracellular matrix (ECM) molecules COL1A1, COL1A2, CTGF, and ACTA2 in TGF-β₁-stimulated healthy skin fibroblasts.

Western Blot Analysis^[1]

Cell Line:

healthy skin fibroblasts treated with TGF-β₁
Concentration:

10 μM
Incubation Time:

72 h
Result:

Decreased the protein expression levels of type I collagen, CCN2, and αSMA in TGF-β₁-stimulated healthy skin fibroblasts.

Real Time qPCR^[1]

Cell Line:

systemic sclerosis (SSc) skin fibroblasts
Concentration:

1, 10 μM
Incubation Time:

6 h, 24 h, 48 h
Result:

Downregulated the mRNA expression levels of COL1A1, COL1A2, CTGF, and ACTA2 in SSc skin fibroblasts in a dose-dependent manner after 48 h.
Significantly reduced mRNA expression of COL1A1, COL1A2, CTGF, ACTA2, FN, and TGF-β₁ compared to untreated cells after 6, 24, or 48 h, with the antifibrotic effect detectable up to 48 h.

ELISA Assay^[1]

Cell Line:

systemic sclerosis (SSc) skin fibroblasts
Concentration:

10 μM
Incubation Time:

24 h
Result:

Significantly increased the levels of HGF and PGE₂ in the culture medium of SSc skin fibroblasts compared to untreated cells.

In Vivo

2ccPA (1-10 mg/kg; i.p.; once daily for 4 weeks) dose-dependently ameliorates Bleomycin hydrochloride (HY-17565A)-induced skin fibrosis in BALB/c mice, with the 10 mg/kg/day dose producing a significant 32% reduction in collagen content and 45% reduction in myofibroblast count^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c (6-week-old female; bleomycin-induced skin fibrosis)^[1]
Dosage:

1, 10 mg/kg
Administration:

i.p.; once daily for 4 weeks
Result:

Reduced skin thickness and collagen content by 22.5% (not significant) at 1 mg/kg/day compared with Bleomycin-only controls.
Reduced skin thickness, reduced collagen content by 32%, and reduced αSMA-positive myofibroblast count by 45% at 10 mg/kg/day compared with Bleomycin-only controls.
Showed no side effects or significant body weight reduction.

Animal Model:

Male C57BL/6J mice^[2]
Dosage:

1.6 mg/kg
Administration:

i.p.; once daily for 5 weeks
Result:

Nearly completely suppressed acute peak demyelination and reduced neuroinflammation.

Related Protocols :

Chemical Information

CAS. Nr. 633278-53-2
Molecular Weight 416.53
Formel C₂₂H₄₁O₅P
SMILES

CCCCCCCC/C=C\CCCCCCCC(OCC1COP(O)(C1)=O)=O
Synonyms

2ccPA18:1
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Higuchi T, et al. Antifibrotic effects of 2-carba cyclic phosphatidic acid (2ccPA) in systemic sclerosis: contribution to the novel treatment. Arthritis Res Ther. 2019 Apr 18;21(1):103. [Content Brief]

[2]. Yamamoto S, et al. Correction to: Protective and therapeutic role of 2-carbacyclic phosphatidic acid in demyelinating disease. J Neuroinflammation. 2018 Mar 5;15(1):67. [Content Brief]

[3]. Hashimoto K, et al. 2-carba cyclic phosphatidic acid suppresses inflammation via regulation of microglial polarisation in the stab-wounded mouse cerebral cortex. Sci Rep. 2018 Jun 26;8(1):9715. [Content Brief]

[4]. Endo M, et al. 2-Carba cyclic phosphatidic acid regulates blood coagulation and fibrinolysis system for repair after brain injury. Brain Res. 2023 Nov 1;1818:148511.

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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