Art. -Nr.: HY-15290

AIM-100

4 Cited Publications

Kundenbewertung

Based on 4 publication(s) in Google Scholar

AIM-100 is a potent and selective Ack1 inhibitor with an IC₅₀ of 21.58 nM. AIM-100 also inhibits Tyr²⁶⁷ phosphorylation. AIM-100 does not inhibits other kinases including PI3-kinase and AKT subfamily members. AIM-100 has an anticancer effect.

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Reinheit: 99.95%
CAS. Nr.: 873305-35-2
Formel: C₂₃H₂₁N₃O₂
Molecular Weight:371.43

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

AIM-100 Chemical Structure

CAS. Nr.: 873305-35-2

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

IC50: 21.58 nM (Ack1)^[2]

Cellular Effect

Cell Line	Type	Value	Description	References
LNCaP	IC₅₀	7 μM Compound: 1b, AIM-100	Anticancer activity against human LNCAP cells assessed as inhibition of cell growth after 72 hrs by trypan blue exclusion assay Anticancer activity against human LNCAP cells assessed as inhibition of cell growth after 72 hrs by trypan blue exclusion assay	[PMID: 25699576]
LNCaP	IC₅₀	7 μM Compound: 1; AIM-100	Antiproliferative activity against human LNCaP cells Antiproliferative activity against human LNCaP cells	[PMID: 34735773]
T-cell	IC₅₀	2.41 μM Compound: 2	Inhibition of anti-CD3/CD28-induced IL2 secretion in human T cells Inhibition of anti-CD3/CD28-induced IL2 secretion in human T cells	[PMID: 17280833]
T-cell	IC₅₀	8.95 μM Compound: 2	Inhibition of T cell activation in human mixed lymphocyte reaction Inhibition of T cell activation in human mixed lymphocyte reaction	[PMID: 17280833]

In Vitro

AIM-100 (2-10 μM; 48 hours) treatment not only inhibits Ack1 activation but also suppresses AKT tyrosine phosphorylation, leading to cell cycle arrest in the G1 phase. AIM-100 not only inhibits Ack1/AKT Tyr-phosphorylation but also suppressed growth of cell lines derived from pancreatic, breast, and lung tumors^[1].
The Ack1 inhibitor AIM-100 not only inhibited Ack1 activity but also was able to suppress AR Tyr²⁶⁷ phosphorylation and its recruitment to the ataxia-telangiectasia mutated kinase (ATM) enhancer^[2].
AIM-100 is able to suppress pTyr²⁶⁷-AR phosphorylation, binding of androgen receptor (AR) to PSA, NKX3.1, and TMPRSS2 promoters, and inhibit AR transcription activity^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

In male nude castrated mice, AIM-100 (4 mg/kg) suppresses growth of radioresistant castration-resistant prostate cancer (CRPC) xenograft tumors by decreasing ataxia-telangiectasia mutated kinase (ATM) expression^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 873305-35-2
Appearance Solid
Molecular Weight 371.43
Formel C₂₃H₂₁N₃O₂
Color White to yellow
SMILES

C1(C2=C(C3=CC=CC=C3)OC4=C2C(NC[C@@H]5CCCO5)=NC=N4)=CC=CC=C1
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (4)

All WBCell Proliferation/Viability Assay

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Journal Impact Factor

Mol Med

Improvement of ACK1-targeted therapy efficacy in lung adenocarcinoma using chloroquine or bafilomycin A1. [Abstract]

2023 Jan 16;29(1):6. PMID: 36647009

AIM-100 purchased from MedChemExpress. Usage Cited in: Mol Med. 2023 Jan 16;29(1):6. [Abstract]

AIM-100 (10, 20 µM; 72 h) significantly reduces the colony formation of A549 cells.

AIM-100 purchased from MedChemExpress. Usage Cited in: Mol Med. 2023 Jan 16;29(1):6. [Abstract]

AIM-100 (10, 20 µM; 24, 48, 72 h) significantly decreases A549 cell proliferation.
Cancer Cell Int

ACK1 condensates promote STAT5 signaling in lung squamous cell carcinoma. [Abstract]

2025 Jun 28;25(1):237. PMID: 40581662
Cell Biochem Funct

Targeted inhibition of ACK1 can inhibit the proliferation of hepatocellular carcinoma cells through the PTEN/AKT/mTOR pathway. [Abstract]

2020 Jul;38(5):642-650. PMID: 32162707
Oncotarget

Ack1 overexpression promotes metastasis and indicates poor prognosis of hepatocellular carcinoma. [Abstract]

2015 Dec 1;6(38):40622-41. PMID: 26536663

AIM-100 purchased from MedChemExpress. Usage Cited in: Oncotarget. 2015 Dec 1;6(38):40622-41. [Abstract]

Ack1 Affects Ser473-phosphorylation of AKT, but not Thr308-phosphorylation in HCC Cells. A. & B. HCC cells are either untreated or pretreated with AIM-100, a Ack1-specific inhibitor, in cell adhesion assays. Upon AIM- 100 treatment, significant loss of p-Ack1 level and a concomitant decrease in Ser473-phosphorylation of AKT level is observed, whereas the effect on Thr308-phosphorylation of AKT levels is not altered.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (134.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6923 mL

13.4615 mL

26.9230 mL

5 mM

0.5385 mL

2.6923 mL

5.3846 mL

10 mM

0.2692 mL

1.3461 mL

2.6923 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Mahajan K, et al. Ack1 tyrosine kinase activation correlates with pancreatic cancer progression. Am J Pathol. 2012 Apr;180(4):1386-93. [Content Brief]

[2]. Mahajan K, et al. Ack1-mediated androgen receptor phosphorylation modulates radiation resistance in castration-resistant prostate cancer. J Biol Chem. 2012 Jun 22;287(26):22112-22. [Content Brief]

[3]. Mahajan K, et al. Effect of Ack1 tyrosine kinase inhibitor on ligand-independent androgen receptor activity. Prostate. 2010 Sep 1;70(12):1274-85. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6923 mL	13.4615 mL	26.9230 mL	67.3074 mL
	5 mM	0.5385 mL	2.6923 mL	5.3846 mL	13.4615 mL
	10 mM	0.2692 mL	1.3461 mL	2.6923 mL	6.7307 mL
	15 mM	0.1795 mL	0.8974 mL	1.7949 mL	4.4872 mL
	20 mM	0.1346 mL	0.6731 mL	1.3461 mL	3.3654 mL
	25 mM	0.1077 mL	0.5385 mL	1.0769 mL	2.6923 mL
	30 mM	0.0897 mL	0.4487 mL	0.8974 mL	2.2436 mL
	40 mM	0.0673 mL	0.3365 mL	0.6731 mL	1.6827 mL
	50 mM	0.0538 mL	0.2692 mL	0.5385 mL	1.3461 mL
	60 mM	0.0449 mL	0.2244 mL	0.4487 mL	1.1218 mL
	80 mM	0.0337 mL	0.1683 mL	0.3365 mL	0.8413 mL
	100 mM	0.0269 mL	0.1346 mL	0.2692 mL	0.6731 mL

Keywords

AIM-100

4 Cited Publications

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AIM-100

Kinase Inhibitor Library

Protein Tyrosine Kinase Compound Library

Anti-Cancer Compound Library

Targeted Diversity Library

Anti-Prostate Cancer Compound Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

Kinase Inhibitor Library Mini

MCE Bioactive Compound Library

Bioactive Compound Library

Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) AIM-100

Biologische Aktivität

Chemical Information

Publications (4)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

AIM-100

Kinase Inhibitor Library

Protein Tyrosine Kinase Compound Library

Anti-Cancer Compound Library

Targeted Diversity Library

Anti-Prostate Cancer Compound Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

Kinase Inhibitor Library Mini

MCE Bioactive Compound Library

Bioactive Compound Library

Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) AIM-100

Biologische Aktivität

Chemical Information

Publications (4)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

AIM-100 Related Classifications

HY-15290 Related Classifications