Art. -Nr.: HY-A0181

Adenosine monophosphate

(Synonyms: AMP)

22 Cited Publications

Kundenbewertung

Based on 22 publication(s) in Google Scholar

Adenosine monophosphate is an adenosine A1 receptor agonist. Adenosine monophosphate has significant antiviral activity against HSV-1 and HSV-2. Adenosine monophosphate is a key cellular metabolite regulating energy homeostasis and signal transduction.

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Reinheit: 99.80%
CAS. Nr.: 61-19-8
Formel: C₁₀H₁₄N₅O₇P
Molecular Weight:347.22

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Adenosine monophosphate Chemical Structure

CAS. Nr.: 61-19-8

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (22)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

Human Endogenous Metabolite

Microbial Metabolite

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

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Alle HSV Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	EC₅₀	>100 μM Compound: AMP	Cytotoxicity against human HCT-116 cells incubated for 48 hrs by MTT assay Cytotoxicity against human HCT-116 cells incubated for 48 hrs by MTT assay	[PMID: 36518704]
HCT-116	EC₅₀	>100 μM Compound: AMP	Cytotoxicity against human HCT-116 cells overexpressing human BCRP incubated for 48 hrs by MTT assay Cytotoxicity against human HCT-116 cells overexpressing human BCRP incubated for 48 hrs by MTT assay	[PMID: 36518704]
HCT-116	EC₅₀	>100 μM Compound: AMP	Cytotoxicity against human HCT-116 cells overexpressing human MDR1 incubated for 48 hrs by MTT assay Cytotoxicity against human HCT-116 cells overexpressing human MDR1 incubated for 48 hrs by MTT assay	[PMID: 36518704]
HEK293	EC₅₀	>100 μM Compound: AMP	Cytotoxicity against human HEK293 cells incubated for 48 hrs by MTT assay Cytotoxicity against human HEK293 cells incubated for 48 hrs by MTT assay	[PMID: 36518704]
HEK-293T	EC₅₀	0.5 μM Compound: 5'-AMP	Agonist activity at human A1AR expressed in HEK293T/17 cells assessed as inhibition of isoproterenol-induced cAMP accumulation incubated for 10 mins by luciferase reporter assay Agonist activity at human A1AR expressed in HEK293T/17 cells assessed as inhibition of isoproterenol-induced cAMP accumulation incubated for 10 mins by luciferase reporter assay	[PMID: 22738238]
HepG2	EC₅₀	1.4 μM Compound: AMP	Allosteric activation of AMPK alpha2/beta1/gamma1 in human HepG2 cells by scintillation proximity assay Allosteric activation of AMPK alpha2/beta1/gamma1 in human HepG2 cells by scintillation proximity assay	[PMID: 31744674]

In Vitro

Adenosine monophosphate is an adenosine A1 receptor agonist^[1]. Adenosine monophosphate-induced cell toxicity is negligible at concentrations of 25 to 400 μM in RAW264.7 cells. Adenosine monophosphate significantly attenuates the mRNA expression of tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 in RAW264.7 cells. The dose-dependence of TNF-α and IL-6 mRNA show that at concentration of 400 μM, Adenosine monophosphate exhibits the maximum inhibition. Exposure of cells to Adenosine monophosphate significantly reduces recruitment of NF-κB p65 to TNF-α, IL-6, and IL-1β gene promoters^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

C57BL/6J mice treated with Adenosine monophosphate markedly increases hepatic adenosine level. Survival rate in PBS-treated mice (n=15) is 60% (8 h) and 33.3% (24 h), whereas survival rate in Adenosine monophosphate-treated mice (n=15) is 100% (8 h) and 93.3% (24 h). Serum aspartate transaminase (AST) and alanine transaminase (ALT) levels are significantly lowered in the Adenosine monophosphate group than in the vehicle group. The area and extent of necrosis is attenuated and the infiltration of inflammatory cells is reduced in the Adenosine monophosphate group^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 61-19-8
Appearance Solid
Molecular Weight 347.22
Formel C₁₀H₁₄N₅O₇P
Color White to off-white
SMILES

O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N2C=NC3=C2N=CN=C3N
Synonyms

AMP
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
- Microorganisms
- Endogenous metabolite
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (22)

All Flow CytometryELISACell Proliferation/Viability AssayCell Imaging/StainingWB

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Journal Impact Factor

Nature

Lithocholic acid phenocopies anti-ageing effects of calorie restriction. [Abstract]

2025 Jul;643(8070):192-200. PMID: 39695227
Cell

Adenosine kinase and ADAL coordinate detoxification of modified adenosines to safeguard metabolism. [Abstract]

2025 Oct 30;188(22):6151-6169.e24. PMID: 40840445
Exploration

2026 Jun 22. PMID: 42382697
Theranostics

Engaging natural regulatory myeloid cells to restrict T-cell hyperactivation-induced liver inflammation via extracellular vesicle-mediated purine metabolism regulation. [Abstract]

2024 Aug 12;14(12):4874-4893. PMID: 39239508
Theranostics

Histone deacetylase inhibitors promote epithelial-mesenchymal transition in Hepatocellular Carcinoma via AMPK-FOXO1-ULK1 signaling axis-mediated autophagy. [Abstract]

2020 Aug 13;10(22):10245-10261. PMID: 32929346
MedComm (2020)

Novel antibiotics against Staphylococcus aureus without detectable resistance by targeting proton motive force and FtsH. [Abstract]

2025 Jan 8;6(1):e70046. PMID: 39781293
J Exp Med

Xbp1 controls the reparative function of intestinal ILC2s during colitis. [Abstract]

2026 Jan 5;223(1):e20250440. PMID: 41165618
Stem Cell Res Ther

CD73-expressing endometrial regenerative cell-derived exosomes mitigate acute cardiac allograft rejection through regulating adenosine metabolism in mice. [Abstract]

2025 Jun 2;16(1):277. PMID: 40457389

Adenosine monophosphate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2025 Jun 2;16(1):277. [Abstract]

Flow cytometry analysis of adenosine A2aR and A2bR expression treated with Adenosine monophosphate (AMP) (100 μM).
Stem Cell Res Ther

Exosomes derived from a mesenchymal-like endometrial regenerative cells ameliorate renal ischemia reperfusion injury through delivery of CD73. [Abstract]

2025 Mar 26;16(1):148. PMID: 40140882
Stem Cell Res Ther

CD73 mediates the therapeutic effects of endometrial regenerative cells in concanavalin A-induced hepatitis by regulating CD4+ T cells. [Abstract]

2023 Sep 29;14(1):277. PMID: 37775797

Adenosine monophosphate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2023 Sep 29;14(1):277. [Abstract]

IFN-γ production was detected by ELISA in the cell culture supernatant, ERCs or NC-ERCs significantly inhibited the differentiation of activated CD4⁺ T cells to Th1 cells compared with CD73-KO-ERCs, as well as the production of IFN-γ, especially in the presence of Adenosine monophosphate (AMP) (50 μM, 72 h).

Adenosine monophosphate purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2023 Sep 29;14(1):277. [Abstract]

Adenosine monophosphate (AMP) (50 μM, 72 h) was introduced to the culture medium to ensure sufficient substrate for CD73 in all situations, the reduction of CD69 expression on CD4⁺ T cells was more apparent when cultured with NC-ERCs than CD73-KO-ERCs.
Cell Chem Biol

The cytotoxic effect of prexasertib is a consequence of dual inhibition on both CHK1 and AMPK. [Abstract]

2026 Jun 18;33(6):767-784.e7. PMID: 42061410
Anal Chem

Ultrahigh Flow Regulated Discharge Coupling with Targeted Mass Spectrometry Analysis: Real Time Capturing Biomolecules in Exhaled Breath. [Abstract]

2025 May 13;97(18):9827-9835. PMID: 40302637
Biochem Pharmacol

Exosomal CD73 from endometrial regenerative cells confers protective effects against ischemia-reperfusion injury in kidney transplantation. [Abstract]

2026 Sep;251(Pt 1):118080. PMID: 42162765
Mar Pollut Bull

Microplastics exposure affects the feeding behavior of the whiteleg shrimp Litopenaeus vannamei by impairing the appetite, olfaction and digestion. [Abstract]

2025 Nov:220:118455. PMID: 40680651
Molecules

Seeking Correlation Among Porin Permeabilities and Minimum Inhibitory Concentrations Through Machine Learning: A Promising Route to the Essential Molecular Descriptors. [Abstract]

2025 Mar 9;30(6):1224. PMID: 40142001
Molecules

Untargeted Metabolomics Using UHPLC-HRMS Reveals Metabolic Changes of Fresh-Cut Potato during Browning Process. [Abstract]

2023 Apr 11;28(8):3375. PMID: 37110608
Genes (Basel)

AMPK Promotes Larval Metamorphosis of Mytilus coruscus. [Abstract]

2022 Dec 16;13(12):2384. PMID: 36553651

Adenosine monophosphate purchased from MedChemExpress. Usage Cited in: Genes (Basel). 2022 Dec 16;13(12):2384. [Abstract]

The Survival rate of M. coruscus larvae after being treated with Adenosine monophosphate (AMP) (0.5, 1. 2.5 mM, 72 h) The figures in the legend represent the concentration (mM).
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
Biochem Biophys Res Commun

Feedback activation of CD73-Adenosine axis attenuates the antitumor immunity of STING pathway. [Abstract]

2024 May 14:708:149814. PMID: 38531218

Adenosine monophosphate purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2024 May 14:708:149814. [Abstract]

The levels of ROS was significantly improved in the AMPD2 overexpression group after treatment with Adenosine monophosphate (AMP) (10 μM, 24 h).

Adenosine monophosphate purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2024 May 14:708:149814. [Abstract]

Lower expression of total p38, phosphorylated p38 and Gpx4 could be observed after Adenosine monophosphate (AMP) (10 μM, 24 h) treatment in the AMPD2 overexpression group detected by western blot analysis.
Res Sq

A phosphorylation switch at MRE11 links ATM-ATR and calcium signaling to safeguard stalled replication fork stability. [Abstract]

2026 Apr 22:rs.3.rs-9192680. PMID: 42078910
University of Cambridge

2023 Feb.
Research Square Preprint

2022 May.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 16.67 mg/mL (48.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 1.67 mg/mL (4.81 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8800 mL

14.3999 mL

28.7999 mL

5 mM

0.5760 mL

2.8800 mL

5.7600 mL

10 mM

0.2880 mL

1.4400 mL

2.8800 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1.67 mg/mL (4.81 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 1.67 mg/mL (4.81 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1.67 mg/mL (4.81 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 3.33 mg/mL (9.59 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Rittiner JE, et al. AMP is an adenosine A1 receptor agonist. J Biol Chem. 2012 Feb 17;287(8):5301-9. [Content Brief]

[2]. Zhan Y, et al. Adenosine 5'-monophosphate ameliorates D-galactosamine/lipopolysaccharide-induced liver injury through an adenosine receptor-independent mechanism in mice. Cell Death Dis. 2014 Jan 9;5:e985. [Content Brief]

[3]. Ayisi NK, et al. Comparison of the antiviral effects of 5-methoxymethyldeoxyuridine-5'-monophosphate with adenine arabinoside-5'-monophosphate. Antiviral Res. 1983 Sep;3(3):161-74. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	2.8800 mL	14.3999 mL	28.7999 mL	71.9996 mL
DMSO	5 mM	0.5760 mL	2.8800 mL	5.7600 mL	14.3999 mL
	10 mM	0.2880 mL	1.4400 mL	2.8800 mL	7.2000 mL
	15 mM	0.1920 mL	0.9600 mL	1.9200 mL	4.8000 mL
	20 mM	0.1440 mL	0.7200 mL	1.4400 mL	3.6000 mL
	25 mM	0.1152 mL	0.5760 mL	1.1520 mL	2.8800 mL
	30 mM	0.0960 mL	0.4800 mL	0.9600 mL	2.4000 mL
	40 mM	0.0720 mL	0.3600 mL	0.7200 mL	1.8000 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Adenosine monophosphate

22 Cited Publications

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Adenosine monophosphate

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Publications Citing Use of MedChemExpress (MCE) Adenosine monophosphate

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Alle Adenosine Receptor Isoform-spezifische Produkte anzeigen

Alle HSV Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (22)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Adenosine monophosphate Related Classifications

HY-A0181 Related Classifications