Art. -Nr.: HY-18633

MDK83190

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MDK83190 is a potent apoptosis activator, induces Apaf-1 oligomerization, increases procaspase-9 processing and subsequent caspase-3 activation in a cyto c-dependent Manner.

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Reinheit: 98.61%
CAS. Nr.: 79183-19-0
Formel: C₁₅H₉Cl₂NO₂
Molecular Weight:306.14

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

MDK83190 Chemical Structure

CAS. Nr.: 79183-19-0

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

MDK83190 is a potent apoptosis activator, induces Apaf-1 oligomerization, increases procaspase-9 processing and subsequent caspase-3 activation in a cyto c-dependent Manner^[1].

IC₅₀ & Target

IC50: 4-9 uM (Leukemia origin cells)^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	>50 μM Compound: 1	Antiproliferative activity against human HCT-116p53KO subline assessed as growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human HCT-116p53KO subline assessed as growth inhibition measured after 72 hrs by MTT assay	[PMID: 34902732]
HCT-116	IC₅₀	38 μM Compound: 1	Antiproliferative activity against human HCT-116 assessed as growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human HCT-116 assessed as growth inhibition measured after 72 hrs by MTT assay	[PMID: 34902732]
K562	IC₅₀	20 μM Compound: 1	Antiproliferative activity against human K562 cells assessed as growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human K562 cells assessed as growth inhibition measured after 72 hrs by MTT assay	[PMID: 34902732]
MCF7	IC₅₀	>50 μM Compound: 1	Antiproliferative activity against human MCF7 cells assessed as growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as growth inhibition measured after 72 hrs by MTT assay	[PMID: 34902732]
MCF7	IC₅₀	>50 μM Compound: 1	Antiproliferative activity against human MCF7 cells assessed as growth inhibition under hypoxia conditions measured after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as growth inhibition under hypoxia conditions measured after 72 hrs by MTT assay	[PMID: 34902732]
MDA-MB-231	IC₅₀	>50 μM Compound: 1	Antiproliferative activity against human MDA-MB-231 cells assessed as growth inhibition under hypoxia conditions measured after 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as growth inhibition under hypoxia conditions measured after 72 hrs by MTT assay	[PMID: 34902732]
MDA-MB-231	IC₅₀	>50 μM Compound: 1	Antiproliferative activity against human MDA-MB-231 cells assessed as growth inhibition under normoxia conditions measured after 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as growth inhibition under normoxia conditions measured after 72 hrs by MTT assay	[PMID: 34902732]

In Vitro

MDK83190 (Compound 2) (1-50 μM; 2 hours) effects against cell lines of lymphoid origin (CCRF-CEM, MOLT-4, and Jurkat) with IC₅₀ values ranging from 4 to 9 μM^[1].
MDK83190 (Compound 2) (20 μM; 30 mins) activates procaspase-9 and procaspase-3 in a cyto c-dependent manner^[1].
MDK83190 (Compound 2) (0.1-100 μM; 22 hours) strongly induces caspase-3 activation, PARP cleavage, and DNA fragmentation, and finally killing cells with an IC₅₀ of 4 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Leukemia cell line: CCRF-CEM cells, MOLT-4 cells, and Jurkat cells
Concentration:

1-50 μM
Incubation Time:

2 hours
Result:

Was sensitive to compound 2-induced apoptosis.

Western Blot Analysis^[1]

Cell Line:

Hela cells
Concentration:

20 μM
Incubation Time:

30 mins
Result:

Induced procaspase-9 and procaspase-3 to active forms.

Apoptosis Analysis^[1]

Cell Line:

Jurkat cells
Concentration:

0.1-100 μM
Incubation Time:

22 hours
Result:

Resulted in apoptosis and finally killed cells.

Chemical Information

CAS. Nr. 79183-19-0
Appearance Solid
Molecular Weight 306.14
Formel C₁₅H₉Cl₂NO₂
Color Yellow to orange
SMILES

O=C1N(CC2=CC=C(Cl)C(Cl)=C2)C3=C(C=CC=C3)C1=O
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : ≥ 46 mg/mL (150.26 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.2665 mL

16.3324 mL

32.6648 mL

5 mM

0.6533 mL

3.2665 mL

6.5330 mL

10 mM

0.3266 mL

1.6332 mL

3.2665 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (8.17 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Nguyen JT, et al. Direct activation of the apoptosis machinery as a mechanism to target cancer cells. Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7533-8. Epub 2003 Jun 13. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.2665 mL	16.3324 mL	32.6648 mL	81.6620 mL
	5 mM	0.6533 mL	3.2665 mL	6.5330 mL	16.3324 mL
	10 mM	0.3266 mL	1.6332 mL	3.2665 mL	8.1662 mL
	15 mM	0.2178 mL	1.0888 mL	2.1777 mL	5.4441 mL
	20 mM	0.1633 mL	0.8166 mL	1.6332 mL	4.0831 mL
	25 mM	0.1307 mL	0.6533 mL	1.3066 mL	3.2665 mL
	30 mM	0.1089 mL	0.5444 mL	1.0888 mL	2.7221 mL
	40 mM	0.0817 mL	0.4083 mL	0.8166 mL	2.0415 mL
	50 mM	0.0653 mL	0.3266 mL	0.6533 mL	1.6332 mL
	60 mM	0.0544 mL	0.2722 mL	0.5444 mL	1.3610 mL
	80 mM	0.0408 mL	0.2042 mL	0.4083 mL	1.0208 mL
	100 mM	0.0327 mL	0.1633 mL	0.3266 mL	0.8166 mL

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MDK83190

Covalent Screening Library

Anti-Blood Cancer Compound Library

Mitochondria-Targeted Compound Library

Cell Death Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Apoptosis Compound Library

Anti-Cancer Compound Library

Peptidomimetic Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

MDK83190

Covalent Screening Library

Anti-Blood Cancer Compound Library

Mitochondria-Targeted Compound Library

Cell Death Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Apoptosis Compound Library

Anti-Cancer Compound Library

Peptidomimetic Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

MDK83190 Related Classifications

HY-18633 Related Classifications