Art. -Nr.: HY-B0450

Ciclopirox

Name: Ciclopirox
Brand: MedChemExpress
SKU: HY-B0450
Price: 55.00 USD
Availability: InStock
Rating: 5 (14 reviews)

(Synonyms: HOE296b)

14 Cited Publications

Kundenbewertung

Based on 14 publication(s) in Google Scholar

Ciclopirox (HOE296b) is a synthetic and orally active antifungal agent that can be used for superficial mycoses reseaech. Ciclopirox olamine has a very broad spectrum of activity and inhibits dermatophytes, yeasts, molds, and many Gram-positive and Gram-negative species pathogenic. Ciclopirox also has anticancer and anti-inflammatory effect.

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Reinheit : 98.25%
CAS. Nr.: 29342-05-0
Formel: C₁₂H₁₇NO₂
Molecular Weight:207.27

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Ciclopirox Chemical Structure

CAS. Nr.: 29342-05-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (14)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Protokoll (2)
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Cellular Effect

Cell Line	Type	Value	Description	References
Astrocyte	IC₅₀	0.33 μM Compound: Ciclopirox	Inhibition of H2O2-induced mitochondrial metabolic dysfunction in Sprague-Dawley rat primary astrocytes by resazurin-dye based presto blue assay Inhibition of H2O2-induced mitochondrial metabolic dysfunction in Sprague-Dawley rat primary astrocytes by resazurin-dye based presto blue assay	[PMID: 28089588]
Astrocyte	IC₅₀	249.9 nM Compound: Ciclopirox	Cytoprotective activity in Sprague-Dawley rat primary astrocytes assessed as inhibition of H2O2-induced cell death by LDH release assay Cytoprotective activity in Sprague-Dawley rat primary astrocytes assessed as inhibition of H2O2-induced cell death by LDH release assay	[PMID: 28089588]
HEK293	IC₅₀	>500 μM Compound: ciclopirox	Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay	[PMID: 23241029]
Vero	CC₅₀	>100 μM Compound: 1; CPX	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 24 hrs by MTS assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 24 hrs by MTS assay	[PMID: 35635945]

In Vitro

Ciclopirox (10 μM, 18 h) inhibits HUVEC proliferation and angiogenesis^[4].
Ciclopirox (0-10 μM, 20 h) inhibits deoxyhypusine hydroxylation in HUVECs^[4].
Ciclopirox (0-40 μM, 72 h) shows anti-tumor activity in H1299 and 95D cells (decreases cell viability, with IC₅₀s of 11.13 and 4.136 μM respectively), and inhibits cell migration and invasion^[5].
Ciclopirox (0-40 μM, 48 h) arrests both H1299 and 95D cells in G1 phase, decreases Cyclin D1 and CDK4 protein level in H1299 and 95D cells^[5].
Ciclopirox (0-20 μM) induces cell aerobic glycolysis, impairs mitochondrial functions and enhances the generation of ROS in H1299 and 95D cells^[5].
Ciclopirox (0-40 μM, 48 h) activates PERK-dependent ER stress in CRC cells (HCT-8, HCT-8/5-FU, and DLD-1 cells)^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

In Vivo

Ciclopirox (20 mg/kg, i.p.) reduces tumor size in mouse H1299 xenograft model, and reduces tumor cell proliferation (Ki67 staining) and increases apoptosis (Cleaved-Caspase 3 and Tunel staining)^[5].
Ciclopirox (25 mg/kg, p.o., daily) also inhibits tumor growth in human breast cancer MDA-MB231 xenografts in mice^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 29342-05-0
Appearance Solid
Molecular Weight 207.27
Formel C₁₂H₁₇NO₂
Color White to off-white
SMILES

CC(C=C(C1CCCCC1)N2O)=CC2=O
Synonyms

HOE296b
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (14)

All WBFlow CytometryRT-PCRCell Proliferation/Viability AssayBio/Physico-chemical AssayIPIn Vivo ImagingIHC

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Journal Impact Factor

Cell Death Dis

A novel taxane SB-T-101141 triggers a noncanonical ferroptosis to overcome Paclitaxel resistance of breast cancer via iron homeostasis-related KHSRP. [Abstract]

2025 May 19;16(1):403. PMID: 40389408
Cell Death Dis

Inhibition of mitochondrial OMA1 ameliorates osteosarcoma tumorigenesis. [Abstract]

2024 Nov 1;15(11):786. PMID: 39487118

Ciclopirox purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 1;15(11):786. [Abstract]

Western blotting analysis of OMA1 and mitophagy-related proteins in OS cells treated with CPX (Ciclopirox, 0-20 μM) for 24 h.

Ciclopirox purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 1;15(11):786. [Abstract]

RT-qPCR analysis of OMA1 mRNA levels in OS cells treated with CPX (Ciclopirox, 0-20 μM) for 24 h.

Ciclopirox purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 1;15(11):786. [Abstract]

Western blotting analysis of OMA1 protein in OS cells co-treated with CPX (20 µM) and a serial dose of MG132 for 24 h.

Ciclopirox purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 1;15(11):786. [Abstract]

Endogenous ubiquitination levels of OMA1 in OS cells treated with CPX (20 µM) for 24 h were determined using an immunoprecipitation assay.

Ciclopirox purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 1;15(11):786. [Abstract]

Representative MRI images of nude mice with right proximal tibia injected control or OMA1-deficient 143B cells for 4 weeks were intraperitoneally injected with 0.9% NaCl or CPX (20 mg/kg) for 2 weeks (n = 3).

Ciclopirox purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 1;15(11):786. [Abstract]

Immunohistochemical analysis of Ki67 and Cleaved-caspase 3 in the OS xenograft intraperitoneally injected with 0.9% NaCl or CPX (20 mg/kg, 2 weeks, ip).
Pharmacol Res

Deoxyhypusine hydroxylase as a novel pharmacological target for ischemic stroke via inducing a unique post-translational hypusination modification. [Abstract]

2022 Feb:176:106046. PMID: 35007708
Adv Healthc Mater

Iron Drives Eosinophil Differentiation in Allergic Airway Inflammation Through Mitochondrial Metabolic Adaptation. [Abstract]

2025 Mar;14(7):e2405085. PMID: 39853900

Ciclopirox purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2025 Mar;14(7):e2405085. [Abstract]

Intervention with CPX (Ciclopirox olamine, 1 μM) on day 4 during BMDE differentiation, followed by assessment of BMDE proportions and counts on day 9.

Ciclopirox purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2025 Mar;14(7):e2405085. [Abstract]

Flow cytometry analysis of cell viability under CPX (Ciclopirox olamine) treatment using DAPI staining.

Ciclopirox purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2025 Mar;14(7):e2405085. [Abstract]

Intervention with Fe²⁺ (10 μM) and DFO or CPX (Ciclopirox olamine) during BMDE differentiation, followed by examination of BMDE counts.
Int J Mol Med

2026 Oct;58(4):273. PMID: 42535365
Clin Transl Med

Iron controls T helper cell pathogenicity by promoting glucose metabolism in autoimmune myopathy. [Abstract]

2022 Aug;12(8):e999. PMID: 35917405
Redox Rep

2026 Dec 31;31(1):2702136. PMID: 42444025
J Agric Food Chem

Zearalenone Regulates Energy Metabolism and Proliferation of Goat Endometrial Epithelial Cells through Estrogen Receptor 1. [Abstract]

2025 Aug 13;73(32):20385-20395. PMID: 40762316
Eur J Pharmacol

Ciclopirox inhibits NLRP3 inflammasome activation via protecting mitochondria and ameliorates imiquimod-induced psoriatic inflammation in mice. [Abstract]

2022 Sep 5:930:175156. PMID: 35868446
Mol Neurobiol

Stress-mediated Activation of Ferroptosis, Pyroptosis, and Apoptosis Following Mild Traumatic Brain Injury Exacerbates Neurological Dysfunctions. [Abstract]

2025 Apr;62(4):4055-4075. PMID: 39388040
mBio

2026 Sep 9;17(9):e0116826. PMID: 42545010
Front Pharmacol

Polyphyllin Ⅲ-Induced Ferroptosis in MDA-MB-231 Triple-Negative Breast Cancer Cells can Be Protected Against by KLF4-Mediated Upregulation of xCT. [Abstract]

2021 May 10:12:670224. PMID: 34040532
ACS Chem Biol

Mass Spectrometry-Based Screening Reveals Inhibitors of Cholesterol 25-Hydroxylase. [Abstract]

2026 Jun 12. PMID: 42284103
SSRN

2025 Dec 4.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (482.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.8246 mL

24.1231 mL

48.2462 mL

5 mM

0.9649 mL

4.8246 mL

9.6493 mL

10 mM

0.4825 mL

2.4123 mL

4.8246 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (12.06 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (12.06 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (12.06 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protokoll

All Histomorphology Signal Pathway Related

Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.
Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Niewerth, M., et al., Ciclopirox olamine treatment affects the expression pattern of Candida albicans genes encoding virulence factors, iron metabolism proteins, and drug resistance factors. Antimicrob Agents Chemother, 2003. 47(6): p. 1805-17. [Content Brief]

[2]. Leem, S.H., et al., The possible mechanism of action of ciclopirox olamine in the yeast Saccharomyces cerevisiae. Mol Cells, 2003. 15(1): p. 55-61. [Content Brief]

[3]. Ratnavel, R.C., R.A. Squire, and G.C. Boorman, Clinical efficacies of shampoos containing ciclopirox olamine (1.5%) and ketoconazole (2.0%) in the treatment of seborrhoeic dermatitis. J Dermatolog Treat, 2007. 18(2): p. 88-96. [Content Brief]

[4]. Clement PM, et al. The antifungal drug ciclopirox inhibits deoxyhypusine and proline hydroxylation, endothelial cell growth and angiogenesis in vitro. Int J Cancer. 2002 Aug 1;100(4):491-8. [Content Brief]

[5]. Lu J, et al. Ciclopirox targets cellular bioenergetics and activates ER stress to induce apoptosis in non-small cell lung cancer cells. Cell Commun Signal. 2022 Mar 24;20(1):37. [Content Brief]

[6]. Zhou H, et al. The antitumor activity of the fungicide ciclopirox. Int J Cancer. 2010 Nov 15;127(10):2467-77. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.8246 mL	24.1231 mL	48.2462 mL	120.6156 mL
	5 mM	0.9649 mL	4.8246 mL	9.6492 mL	24.1231 mL
	10 mM	0.4825 mL	2.4123 mL	4.8246 mL	12.0616 mL
	15 mM	0.3216 mL	1.6082 mL	3.2164 mL	8.0410 mL
	20 mM	0.2412 mL	1.2062 mL	2.4123 mL	6.0308 mL
	25 mM	0.1930 mL	0.9649 mL	1.9298 mL	4.8246 mL
	30 mM	0.1608 mL	0.8041 mL	1.6082 mL	4.0205 mL
	40 mM	0.1206 mL	0.6031 mL	1.2062 mL	3.0154 mL
	50 mM	0.0965 mL	0.4825 mL	0.9649 mL	2.4123 mL
	60 mM	0.0804 mL	0.4021 mL	0.8041 mL	2.0103 mL
	80 mM	0.0603 mL	0.3015 mL	0.6031 mL	1.5077 mL
	100 mM	0.0482 mL	0.2412 mL	0.4825 mL	1.2062 mL

Bioz

Keywords

Ciclopirox

14 Cited Publications

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Ciclopirox

Publications Citing Use of MedChemExpress (MCE) Ciclopirox