Diclofenac
Based on 24 publication(s) in Google Scholar
Diclofenac is a potent and nonselective anti-inflammatory agent, acts as a COX inhibitor, with IC50s of 4 and 1.3 nM for human COX-1 and COX-2 in CHO cells, and 5.1 and 0.84 μM for ovine COX-1 and COX-2, respectively. Diclofenac induces apoptosis of neural stem cells (NSCs) via the activation of the caspase cascade.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.92%
- CAS. Nr.: 15307-86-5
- Formel: C14H11Cl2NO2
- Molecular Weight:296.15
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months
Publications Citing Use of MedChemExpress (MCE) Diclofenac
More- Adv Funct Mater. 2026 Feb 3.
- J Hazard Mater. 2015 May 30;289:18-27. [Abstract]
- Biomater Res. 2025 Nov 7:29:0280. [Abstract]
- Int J Biol Macromol. 2026 Mar:352:151224. [Abstract]
- Chemosphere. 2019 Jun:225:378-387. [Abstract]
- Emerg Contam. 2026 Feb 23.
- Cell Rep. 2025 Feb 7;44(2):115263. [Abstract]
- Anal Chem. 2025 Jun 3;97(21):11099-11109. [Abstract]
- Ecotoxicol Environ Saf. 2025 Nov 15:307:119433. [Abstract]
- Chem Biol Interact. 2021 Apr 1:338:109425. [Abstract]
- Int J Mol Sci. 2022 Oct 11;23(20):12066. [Abstract]
- J Phys Chem Solids. 2017 October;109:117-123.
- Cell Rep Methods. 2023 Oct 23;3(10):100599. [Abstract]
- Pharm Res. 2022 Oct;39(10):2515-2527. [Abstract]
- ACS Infect Dis. 2024 Aug 9;10(8):2860-2869. [Abstract]
- Biotechnol Bioeng. 2021 Dec;118(12):4687-4698. [Abstract]
- J Pharm Biomed Anal. 2021 Jul 15:201:114105. [Abstract]
- Phys Chem Chem Phys. 2016 Jan 21;18(3):1526-36. [Abstract]
- Toxicol Mech Methods. 2019 Nov;29(9):654-664. [Abstract]
- Arch Microbiol. 2024 Jun 7;206(7):289. [Abstract]
- Eur J Drug Metab Pharmacokinet. 2022 Sep;47(5):639-652. [Abstract]
- Thai Journal of Pharmaceutical Sciences. 2025 Dec.
- bioRxiv. 2024 Apr 7.
- Federal University of Rio Grande do Sul. 2017 Nov.
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Cell Proliferation/Viability Assay
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Flow Cytometry
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WB
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Cell Imaging/Staining
Biologische Aktivität
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Human COX-2 1.3 nM (IC50, in CHO cells) |
Human COX-1 4 nM (IC50, in CHO cells) |
Ovine COX-2 0.84 μM (IC50) |
Ovine COX-1 5.1 μM (IC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CHO | IC50 |
4 μM
Compound: Diclofenac
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TP_TRANSPORTER: inhibition of Adefovir uptake in OAT1-expressing CHO cells
TP_TRANSPORTER: inhibition of Adefovir uptake in OAT1-expressing CHO cells
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[PMID: 10991954] |
| Erythrocyte | IC50 |
0.942 μM
Compound: Diclofenac
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Anti-inflammatory activity in human RBC assessed as membrane stabilization
Anti-inflammatory activity in human RBC assessed as membrane stabilization
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[PMID: 28818769] |
| HEK293 | EC50 |
56 μM
Compound: DCF
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Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by Fluo-4-AM dye based fluorescence assay
Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by Fluo-4-AM dye based fluorescence assay
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[PMID: 30878828] |
| HEK293 | IC50 |
>500 μM
Compound: diclofenac
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Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay
Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay
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[PMID: 23241029] |
| HUVEC | IC50 |
0.75 mM
Compound: Diclofenac
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Antiinflammatory activity in HUVEC assessed as inhibition of LPS-induced ICAM1 expression by cell-ELISA
Antiinflammatory activity in HUVEC assessed as inhibition of LPS-induced ICAM1 expression by cell-ELISA
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10.1039/C0MD00262C |
| L1.2 | IC50 |
>10 μM
Compound: 1
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Inhibition of CXCR1 Lys99Ala mutant transfected in mouse L1.2 cells assessed as inhibition of CXCL8-induced cell migration pretreated for 15 mins measured after 4 hrs
Inhibition of CXCR1 Lys99Ala mutant transfected in mouse L1.2 cells assessed as inhibition of CXCL8-induced cell migration pretreated for 15 mins measured after 4 hrs
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[PMID: 19560921] |
| L1.2 | IC50 |
12 nM
Compound: 1
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Inhibition of wild type CXCR1 transfected in mouse L1.2 cells assessed as inhibition of CXCL8-induced cell migration pretreated for 15 mins measured after 4 hrs
Inhibition of wild type CXCR1 transfected in mouse L1.2 cells assessed as inhibition of CXCL8-induced cell migration pretreated for 15 mins measured after 4 hrs
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[PMID: 19560921] |
| RAW264.7 | IC50 |
10.18 μM
Compound: Diclofenac
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Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced IL-6 production preincubated for 2 hrs followed by LPS stimulation and measured after 20 hrs by ELISA
Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced IL-6 production preincubated for 2 hrs followed by LPS stimulation and measured after 20 hrs by ELISA
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[PMID: 37611534] |
| RAW264.7 | IC50 |
10.45 μM
Compound: Diclofenac
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Antiinflammatory activity in LPS-activated mouse RAW264.7 cells assessed as inhibition of TNF-alpha production preincubated for 2 hrs followed by LPS addition measured after 20 hrs by ELISA method
Antiinflammatory activity in LPS-activated mouse RAW264.7 cells assessed as inhibition of TNF-alpha production preincubated for 2 hrs followed by LPS addition measured after 20 hrs by ELISA method
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[PMID: 37182334] |
| RAW264.7 | IC50 |
19.59 μM
Compound: Diclofenac
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Antiinflammatory activity in LPS-activated mouse RAW264.7 cells assessed as inhibition of IL-6 production preincubated for 2 hrs followed by LPS addition measured after 20 hrs by ELISA method
Antiinflammatory activity in LPS-activated mouse RAW264.7 cells assessed as inhibition of IL-6 production preincubated for 2 hrs followed by LPS addition measured after 20 hrs by ELISA method
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[PMID: 37182334] |
| RAW264.7 | IC50 |
21.34 μM
Compound: Diclofenac
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Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha production preincubated for 2 hrs followed by LPS stimulation and measured after 20 hrs by ELISA
Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha production preincubated for 2 hrs followed by LPS stimulation and measured after 20 hrs by ELISA
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[PMID: 37611534] |
| RAW264.7 | IC50 |
25.34 μM
Compound: Diclofenac
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Antiinflammatory activity in LPS-activated mouse RAW264.7 cells assessed as inhibition of NO production preincubated for 2 hrs followed by LPS addition measured after 20 hrs by DAF-FM staining based assay
Antiinflammatory activity in LPS-activated mouse RAW264.7 cells assessed as inhibition of NO production preincubated for 2 hrs followed by LPS addition measured after 20 hrs by DAF-FM staining based assay
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[PMID: 37182334] |
| RAW264.7 | IC50 |
26.26 μM
Compound: Diclofenac
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Antiinflammatory activity in LPS-activated mouse RAW264.7 cells assessed as inhibition of ROS production by DCFH-DA staining based assay
Antiinflammatory activity in LPS-activated mouse RAW264.7 cells assessed as inhibition of ROS production by DCFH-DA staining based assay
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[PMID: 37182334] |
| RAW264.7 | IC50 |
50.08 μM
Compound: Diclofenac
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Antioxidant activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced ROS production preincubated with compound for 2 hrs followed by LPS stimulation and measured after 20 hrs by DCFH-DA probe based fluorescence analysis
Antioxidant activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced ROS production preincubated with compound for 2 hrs followed by LPS stimulation and measured after 20 hrs by DCFH-DA probe based fluorescence analysis
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[PMID: 37611534] |
| RAW264.7 | IC50 |
54.66 μM
Compound: Diclofenac
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Antioxidant activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated with compound for 2 hrs followed by LPS stimulation and measured after 20 hrs by fluorescence microscopic analysis
Antioxidant activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated with compound for 2 hrs followed by LPS stimulation and measured after 20 hrs by fluorescence microscopic analysis
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[PMID: 37611534] |
| Sf21 | IC50 |
23.62 μM
Compound: Diclofenac
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Inhibition of human recombinant COX2 expressed in baculovirus infected SF21 cell
Inhibition of human recombinant COX2 expressed in baculovirus infected SF21 cell
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[PMID: 20974503] |
| Sf9 | IC50 |
0.03 μM
Compound: diclofenac
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Inhibition of human COX1 expressed in sf9 cells
Inhibition of human COX1 expressed in sf9 cells
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[PMID: 16252917] |
| Sf9 | IC50 |
0.07 μM
Compound: diclofenac
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Inhibition of human COX2 expressed in sf9 cells
Inhibition of human COX2 expressed in sf9 cells
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[PMID: 16252917] |
| THP-1 | IC50 |
143 μM
Compound: Diclofenac
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Inhibition of PMA-induced human THP1 monocytes differentiation in to macrophages assessed as macrophage metabolic activity preincubated for 6 hrs before PMA stimulation
Inhibition of PMA-induced human THP1 monocytes differentiation in to macrophages assessed as macrophage metabolic activity preincubated for 6 hrs before PMA stimulation
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[PMID: 30818268] |
| THP-1 | IC50 |
4.86 μM
Compound: Diclofenac
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Inhibition of PMA-induced human THP1 monocytes differentiation in to macrophages preincubated for 6 hrs followed by PMA stimulation by phase contrast microscopic method
Inhibition of PMA-induced human THP1 monocytes differentiation in to macrophages preincubated for 6 hrs followed by PMA stimulation by phase contrast microscopic method
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[PMID: 29289887] |
| Ventricular myocyte | IC50 |
12.89 μM
Compound: Diclofenac
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Inhibition of L-type calcium channel measured using whole-cell patch clamp in rat ventricular myocytes
Inhibition of L-type calcium channel measured using whole-cell patch clamp in rat ventricular myocytes
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[PMID: 22761000] |
Diclofenac effectively blocks COX-1 mediated prostanoid production from U937 cell microsomes, with an IC50 of 7±3 nM[1].
Diclofenac (1-60 μM; 1 day) induces neural stem cells (NSCs)death in a concentration-dependent manner[3].
Diclofenac (10-60 μM; 6 hours) increases the expression of cleaved (activated) caspase-3[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Neural stem cells (NSCs)
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Concentration:1, 3, 10, 30, 60 μM
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Incubation Time:1 day
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Result:Induction of cell death was concentration-dependent and the effect was not saturated at a concentration of up to 60 μM.
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Cell Line:Neural stem cells (NSCs)
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Concentration:10, 30 or 60 μM
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Incubation Time:6 hours
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Result:The activation of caspase-3 was increased in a concentration-dependent manner.
Diclofenac (10 mg/kg; administered via oral route just prior to induction of inflammation) shows in vivo anti-inflammatory activity in Wistar rats[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Sprague-Dawley rats (150±200 g)[1]
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Dosage:3 mg/kg
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Administration:Oral administration, b.i.d., for 5 days
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Result:Resulted in a significant increase in faecal 51Cr excretion.
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Animal Model:Wistar rats (150-175 g) bearing Formalin-induced rat foot paw edema model[2]
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Dosage:10 mg/kg
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Administration:Administered via oral route just prior to induction of inflammation
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Result:Showed in vivo anti-inflammatory activity (% edema inhibition=29.2, 1 h; 22.2, 3 h; 20, 6 h).
Chemical Information
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CAS. Nr. 15307-86-5
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Appearance Solid
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Molecular Weight 296.15
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Formel C14H11Cl2NO2
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Color White to light yellow
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SMILES
O=C(O)CC1=CC=CC=C1NC2=C(Cl)C=CC=C2Cl
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 1 year -20°C 6 months
Publications (24)
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Journal Impact Factor
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Most Recent
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J Hazard Mater
Microwave-assisted activated carbon from cocoa shell as adsorbent for removal of sodium diclofenac and nimesulide from aqueous effluents. [Abstract]2015 May 30;289:18-27. PMID: 25702636 -
Biomater Res
Distinct Roles of Matrigel Enabled the Production of Expandable Hepatoblast and Polarized Hepatocyte Organoids from Human Embryonic Stem Cells under 3-Dimensional Suspension Conditions. [Abstract]2025 Nov 7:29:0280. PMID: 41209312 -
Int J Biol Macromol
Reticuline isomerase AKR1B1 with aldo-keto reductase activity and detoxification function from the insect Blaps rhynchopetera. [Abstract]2026 Mar:352:151224. PMID: 41791543 -
Chemosphere
Mass-balance-model-based evaluation of sewage treatment plant contribution to residual pharmaceuticals in environmental waters. [Abstract]2019 Jun:225:378-387. PMID: 30884299 -
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Cell Rep
Spatial proteomics identifies a CRTC-dependent viral signaling pathway that stimulates production of interleukin-11. [Abstract]2025 Feb 7;44(2):115263. PMID: 39921859 -
Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576 -
Ecotoxicol Environ Saf
PPARγ-responsive luciferase reporter system for high-throughput screening of chemical toxins with potential pulmonary fibrosis effects. [Abstract]2025 Nov 15:307:119433. PMID: 41273832 -
Chem Biol Interact
Regulatory effects of non-steroidal anti-inflammatory drugs on cardiac ion channels Nav1.5 and Kv11.1. [Abstract]2021 Apr 1:338:109425. PMID: 33617802 -
Int J Mol Sci
Diclofenac Sensitizes Signet Ring Cell Gastric Carcinoma Cells to Cisplatin by Activating Autophagy and Inhibition of Survival Signal Pathways. [Abstract]2022 Oct 11;23(20):12066. PMID: 36292923
Diclofenac purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 11;23(20):12066. [Abstract]
KATOIII and KATO/DDP were treated with different concentration of Diclofenac (DCF) (0–1000 µM) for 48 h, and cell viability was assessed.
Diclofenac purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 11;23(20):12066. [Abstract]
The KATO/DDP cells were treated with Cisplatin alone or cisplatin in combination with Diclofenac (DCF) at 10 or 20 µM for 24 h, at 37 °C. The apoptosis cells were detected by using annexin V and PI staining and analyzed by flow cytometer.
Diclofenac purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 11;23(20):12066. [Abstract]
The combination effect of Diclofenac (DCF) (0, 5, 10, 20 μM) and Cisplatin on the expression of cleaved PARP in KATO/DDP cells was examined by Western blot analysis.
Diclofenac purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 11;23(20):12066. [Abstract]
The formation of autophagic vacuoles in KATO/DDP cells increased from 27% to 39% and 45% in cotreatments involving cisplatin and Diclofenac (DCF) at 10 and 20 μM, respectively, when compared with treatment of cisplatin alone.
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Cell Rep Methods
RECOVER identifies synergistic drug combinations in vitro through sequential model optimization. [Abstract]2023 Oct 23;3(10):100599. PMID: 37797618 -
Pharm Res
Effect of Salt Form on Gelation and Drug Delivery Properties of Diclofenac-Loaded Poloxamer Gels for Delivery to Impaired Skin. [Abstract]2022 Oct;39(10):2515-2527. PMID: 36002613 -
ACS Infect Dis
Diclofenac Sodium Restores the Sensitivity of Colistin-Resistant Gram-Negative Bacteria to Colistin. [Abstract]2024 Aug 9;10(8):2860-2869. PMID: 39073350 -
Biotechnol Bioeng
An integrated biomimetic array chip for establishment of collagen-based 3D primary human hepatocyte model for prediction of clinical drug-induced liver injury. [Abstract]2021 Dec;118(12):4687-4698. PMID: 34478150 -
J Pharm Biomed Anal
A validated UHPLC-MS/MS method for simultaneous determination of lumiracoxib and its hydroxylation and acyl glucuronidation metabolites in rat plasma: Application to a pharmacokinetic study. [Abstract]2021 Jul 15:201:114105. PMID: 33991809 -
Phys Chem Chem Phys
Adsorption of sodium diclofenac on graphene: a combined experimental and theoretical study. [Abstract]2016 Jan 21;18(3):1526-36. PMID: 26671178 -
Toxicol Mech Methods
Evaluation of 3D re-cellularized tissue engineering: a drug-induced hepatotoxicity model for hepatoprotectant research. [Abstract]2019 Nov;29(9):654-664. PMID: 31345115 -
Arch Microbiol
Diclofenac sodium effectively inhibits the biofilm formation of Staphylococcus epidermidis. [Abstract]2024 Jun 7;206(7):289. PMID: 38847838 -
Eur J Drug Metab Pharmacokinet
Evaluation of an Ussing Chamber System Equipped with Rat Intestinal Tissues to Predict Intestinal Absorption and Metabolism in Humans. [Abstract]2022 Sep;47(5):639-652. PMID: 35733077 -
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Lösungsmittel & Löslichkeit
DMSO : 125 mg/mL (422.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.02 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (281 KB)
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SDS (788 KB)
- English - EN (788 KB)
- Français - FR (788 KB)
- Deutsch - DE (788 KB)
- Norwegian - NO (788 KB)
- Español - ES (788 KB)
- Swedish - SV (788 KB)
- Italian - IT (788 KB)
- Korean - KR (788 KB)
- Portuguese - PT (788 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Riendeau D, et al. Biochemical and pharmacological profile of a tetrasubstituted furanone as a highly selective COX-2 inhibitor. Br J Pharmacol. 1997 May;121(1):105-17. [Content Brief]
[2]. Labib MB, et al. Design, synthesis of novel isoindoline hybrids as COX-2 inhibitors: Anti-inflammatory, analgesic activities and docking study. Bioorg Chem. 2018 Oct;80:70-80. [Content Brief]
[3]. Chiho Kudo, et al. Diclofenac Inhibits Proliferation and Differentiation of Neural Stem Cells. Biochem Pharmacol. 2003 Jul 15;66(2):289-95. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3767 mL | 16.8833 mL | 33.7667 mL | 84.4167 mL |
| 5 mM | 0.6753 mL | 3.3767 mL | 6.7533 mL | 16.8833 mL | |
| 10 mM | 0.3377 mL | 1.6883 mL | 3.3767 mL | 8.4417 mL | |
| 15 mM | 0.2251 mL | 1.1256 mL | 2.2511 mL | 5.6278 mL | |
| 20 mM | 0.1688 mL | 0.8442 mL | 1.6883 mL | 4.2208 mL | |
| 25 mM | 0.1351 mL | 0.6753 mL | 1.3507 mL | 3.3767 mL | |
| 30 mM | 0.1126 mL | 0.5628 mL | 1.1256 mL | 2.8139 mL | |
| 40 mM | 0.0844 mL | 0.4221 mL | 0.8442 mL | 2.1104 mL | |
| 50 mM | 0.0675 mL | 0.3377 mL | 0.6753 mL | 1.6883 mL | |
| 60 mM | 0.0563 mL | 0.2814 mL | 0.5628 mL | 1.4069 mL | |
| 80 mM | 0.0422 mL | 0.2110 mL | 0.4221 mL | 1.0552 mL | |
| 100 mM | 0.0338 mL | 0.1688 mL | 0.3377 mL | 0.8442 mL |