Art. -Nr.: HY-15249

JZL 184

Name: JZL 184
Brand: MedChemExpress
SKU: HY-15249
Price: 42.00 USD
Availability: InStock
Rating: 5 (9 reviews)

9 Cited Publications

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Based on 9 publication(s) in Google Scholar

JZL 184 is a potent, selective and irreversible MAGL inhibitor that blocks 2-Arachidonoylglycerol (2-AG) hydrolysis in brain membranes (IC₅₀ of 8 nM). JZL 184 displays >300-fold selectivity for MAGL over FAAH.

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Reinheit : 99.46%
CAS. Nr.: 1101854-58-3
Formel: C₂₇H₂₄N₂O₉
Molecular Weight:520.49

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

JZL 184 Chemical Structure

CAS. Nr.: 1101854-58-3

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (9)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

IC50: 8 nM (2-Arachidonoylglycerol (2-AG) hydrolysis)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
COS-7	IC₅₀	0.002 μM Compound: 3, JZL184	Inhibition of human MAGL expressed in monkey COS7 cells Inhibition of human MAGL expressed in monkey COS7 cells	[PMID: 23455058]
COS-7	IC₅₀	0.008 μM Compound: 3, JZL184	Inhibition of recombinant MAGL (unknown origin) expressed in monkey COS7 cells Inhibition of recombinant MAGL (unknown origin) expressed in monkey COS7 cells	[PMID: 23455058]
COS-7	IC₅₀	0.025 μM Compound: 3, JZL184	Inhibition of rat MAGL expressed in monkey COS7 cells Inhibition of rat MAGL expressed in monkey COS7 cells	[PMID: 23455058]
COS-7	IC₅₀	2 nM Compound: JZL184	Inhibition of human MAGL expressed in COS7 cells using endogenous 2-AG substrate Inhibition of human MAGL expressed in COS7 cells using endogenous 2-AG substrate	[PMID: 22738638]
COS-7	IC₅₀	4 nM Compound: 6, JZL184	Inhibition of recombinant FAAH expressed in Cos7 cells after 30 mins by using anandamide as a substrate by LC-MS method Inhibition of recombinant FAAH expressed in Cos7 cells after 30 mins by using anandamide as a substrate by LC-MS method	[PMID: 19029917]
COS-7	IC₅₀	6 nM Compound: 6, JZL184	Inhibition of recombinant MAGL expressed in Cos7 cells after 30 mins by using 2-AG as a substrate by LC-MS method Inhibition of recombinant MAGL expressed in Cos7 cells after 30 mins by using 2-AG as a substrate by LC-MS method	[PMID: 19029917]
COS-7	IC₅₀	8 nM Compound: 3, JZL184	Inhibition of mouse recombinant MAGL expressed in african green monkey COS7 cells assessed as inhibition of 2-AG hydrolysis after 30 mins by LC-MS analysis Inhibition of mouse recombinant MAGL expressed in african green monkey COS7 cells assessed as inhibition of 2-AG hydrolysis after 30 mins by LC-MS analysis	[PMID: 21982493]

In Vitro

JZL 184 displays IC₅₀ values of 8 nM and 4 μM for blockade of 2-AG and oleamide (FAAH substrate) hydrolysis in brain membranes, respectively. Comparable inhibitory effects are observed with recombinant MAGL and FAAH when expressed in COS7 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .

In Vivo

JZL 184 (4-40 mg/kg; intraperitoneal injection; once; C57Bl/6 mice) treatment produces a rapid and sustained blockade of brain 2-AG hydrolase activity in mice, resulting in 8-fold elevations in endogenous 2-AG levels that are maintained for at least 8 h. JZL 184-treated mice shows a remarkable array of CB1-dependent behavioral effects, including analgesia, hypomotility, and hypothermia^[1].
JZL 184 prolongs depolarization-induced suppression of excitation (DSE) in Purkinje neurons in cerebellar slices and DSE inhibition (DSI) in CA1 pyramidal neurons in hippocampal slices. JZL 184 produces greater enhancement of DSE/DSI in mouse neurons than that in rat neurons^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male C57Bl/6 mice (6-8 weeks old, 20-26 g)^[1]
Dosage:

4 mg/kg, 8 mg/kg, 16 mg/kg, 40 mg/kg
Administration:

Intraperitoneal injection; once
Result:

Produced a rapid and sustained blockade of brain 2-AG hydrolase activity in mice, resulting in 8-fold elevations in endogenous 2-AG levels that were maintained for at least 8 h.

Chemical Information

CAS. Nr. 1101854-58-3
Appearance Solid
Molecular Weight 520.49
Formel C₂₇H₂₄N₂O₉
Color White to off-white
SMILES

OC(C1=CC=C(OCO2)C2=C1)(C3CCN(C(OC4=CC=C([N+]([O-])=O)C=C4)=O)CC3)C5=CC(OCO6)=C6C=C5
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (9)

All In Vivo Efficacy StudyCell Imaging/StainingFlow CytometryWB

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Journal Impact Factor

Research (Wash D C)

Endocannabinoids Block Headache and Anxiety Comorbidity via Two-Pronged Anterior Insular Projections. [Abstract]

2025 Dec 9:8:1031. PMID: 41377021

JZL 184 purchased from MedChemExpress. Usage Cited in: Research (Wash D C). 2025 Dec 9:8:1031. [Abstract]

JZL 184 (10 mg/kg; i.p.; 2 h) markedly improved both cephalic cutaneous allodynia and anxiety-like behaviors of ISDN-injected mice.
Int J Biol Sci

Stearoyl-CoA Desaturase-1 dependent lipid droplets accumulation in cancer-associated fibroblasts facilitates the progression of lung cancer. [Abstract]

2022 Oct 18;18(16):6114-6128. PMID: 36439884

JZL 184 purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Oct 18;18(16):6114-6128. [Abstract]

JZL 184 (iMAGL) (20 nM; 12 h) significantly downregulated the number of LDs in fibroblasts (NIH 3T3 and MEF).

JZL 184 purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Oct 18;18(16):6114-6128. [Abstract]

The BODIPY staining (FITC) of fibroblasts (NIH 3T3 and MEF) that were treated with/without sodium oleate (50 µM) and JZL 184 (iMAGL) (20 nM; 12 h).

JZL 184 purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Oct 18;18(16):6114-6128. [Abstract]

The protein expression of α-SMA and COL1A2 in fibroblasts (NIH 3T3 and MEF) that were treated with/without sodium oleate (50 µM) and JZL 184 (iMAGL) (20 nM; 24 h).
Oncogene

Monoacylglycerol lipase blockades the senescence-associated secretory phenotype by interfering with NF-κB activation and promotes docetaxel efficacy in prostate cancer. [Abstract]

2024 Sep;43(38):2835-2849. PMID: 39155296
Free Radic Biol Med

Inhibition of MAGL ameliorates glucocorticoid-induced bone loss by regulating the Nrf2 signaling. [Abstract]

2025 Nov 14:243:67-81. PMID: 41242471

JZL 184 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Nov 14:243:67-81. [Abstract]

JZL 184 (16 mg/kg; i.p.; once daily for 16 d) ameliorated GC-induced bone loss in C57BL/6J mice.
Cells

An Epistatic Interaction between Pnpla2 and Lipe Reveals New Pathways of Adipose Tissue Lipolysis. [Abstract]

2019 Apr 29;8(5):395. PMID: 31035700
Cancer Metab

Carboxylesterase 1-mediated endocannabinoid metabolism in skin: role in melanoma progression in BRafV600E/Pten-/- mice. [Abstract]

2025 Feb 11;13(1):8. PMID: 39934865
Inflammation

MAGL Inhibition Relieves Psoriasiform Inflammation and Pruritus Via Modulation of ALOX12-12-HETE Axis in Mice. [Abstract]

2026 Mar 3;49(1):92. PMID: 41774275
bioRxiv

2026 Mar 16.
Research Square Preprint

2024 Oct 18.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : ≥ 35 mg/mL (67.24 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9213 mL

9.6063 mL

19.2127 mL

5 mM

0.3843 mL

1.9213 mL

3.8425 mL

10 mM

0.1921 mL

0.9606 mL

1.9213 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.80 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (4.80 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.80 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Long JZ, et al. Selective blockade of 2-arachidonoylglycerol hydrolysis produces cannabinoid behavioral effects. Nat Chem Biol. 2009 Jan;5(1):37-44. [Content Brief]

[2]. Pan B, et al. Blockade of 2-arachidonoylglycerol hydrolysis by selective monoacylglycerol lipase inhibitor 4-nitrophenyl 4-(dibenzo[d][1,3]dioxol-5-yl(hydroxy)methyl)piperidine-1-carboxylate (JZL184) Enhances retrograde endocannabinoid signaling. J Pharmacol Exp Ther. 2009 Nov;331(2):591-7. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.9213 mL	9.6063 mL	19.2127 mL	48.0317 mL
	5 mM	0.3843 mL	1.9213 mL	3.8425 mL	9.6063 mL
	10 mM	0.1921 mL	0.9606 mL	1.9213 mL	4.8032 mL
	15 mM	0.1281 mL	0.6404 mL	1.2808 mL	3.2021 mL
	20 mM	0.0961 mL	0.4803 mL	0.9606 mL	2.4016 mL
	25 mM	0.0769 mL	0.3843 mL	0.7685 mL	1.9213 mL
	30 mM	0.0640 mL	0.3202 mL	0.6404 mL	1.6011 mL
	40 mM	0.0480 mL	0.2402 mL	0.4803 mL	1.2008 mL
	50 mM	0.0384 mL	0.1921 mL	0.3843 mL	0.9606 mL
	60 mM	0.0320 mL	0.1601 mL	0.3202 mL	0.8005 mL

Bioz

Keywords

JZL 184

9 Cited Publications

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JZL 184

Publications Citing Use of MedChemExpress (MCE) JZL 184