Art. -Nr.: HY-12512

cGAMP

(Synonyms: Cyclic GMP-AMP; 3',3'-cGAMP)

27 Cited Publications

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Based on 27 publication(s) in Google Scholar

cGAMP (Cyclic GMP-AMP) functions as an endogenous second messenger in metazoans and triggers interferon production in response to cytosolic DNA. cGAMP activates stimulator of interferon genes (STING), which activates a signaling cascade leading to the production of type I interferons and other immune mediators.

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Reinheit: 98.47%
CAS. Nr.: 849214-04-6
Formel: C₂₀H₂₄N₁₀O₁₃P₂
Molecular Weight:674.41

Speicherung:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

cGAMP Chemical Structure

CAS. Nr.: 849214-04-6

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (27)
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
H69AR	EC₅₀	4.74 μM Compound: 3',3'-cGAMP	Activation of wild-type human STING expressed in human THP1-Dual cells co-expressing IRF3-activated ISRE assessed as IRF3 reporter activation measured after 7 hrs by luciferase reporter gene assay Activation of wild-type human STING expressed in human THP1-Dual cells co-expressing IRF3-activated ISRE assessed as IRF3 reporter activation measured after 7 hrs by luciferase reporter gene assay	[PMID: 34019405]
H69AR	EC₅₀	58.46 μM Compound: 3',3'-cGAMP	Activation of wild-type human STING expressed in digitonin treated human THP1-Dual cells co-expressing IRF3-activated ISRE assessed as IRF3 reporter activation measured after 30 mins by luciferase reporter gene assay Activation of wild-type human STING expressed in digitonin treated human THP1-Dual cells co-expressing IRF3-activated ISRE assessed as IRF3 reporter activation measured after 30 mins by luciferase reporter gene assay	[PMID: 34019405]
L929	EC₅₀	20 nM Compound: 3',3'-cGAMP	Inhibition of STING in mouse L929 cells assessed as reduction in cGAMP induce IFNbeta expression measured after 4 hrs by qRT-PCR Inhibition of STING in mouse L929 cells assessed as reduction in cGAMP induce IFNbeta expression measured after 4 hrs by qRT-PCR	[PMID: 32206239]
PBMC	EC₅₀	>300 μM Compound: 3',3'-cGAMP	Induction of TNF-alpha production in healthy human PBMC incubated for 16 hrs Induction of TNF-alpha production in healthy human PBMC incubated for 16 hrs	[PMID: 34019405]
PBMC	EC₅₀	15.14 μM Compound: 3',3'-cGAMP	Induction of INF-gamma production in healthy human PBMC incubated for 16 hrs Induction of INF-gamma production in healthy human PBMC incubated for 16 hrs	[PMID: 34019405]
PBMC	EC₅₀	17.25 μM Compound: 3'3'-cGAMP	Induction of IFNgamma secretion in human PBMC incubated for 16 hrs Induction of IFNgamma secretion in human PBMC incubated for 16 hrs	[PMID: 36201304]
PBMC	EC₅₀	18.72 μM Compound: 3'3'-cGAMP	Induction of TNFalpha secretion in human PBMC incubated for 16 hrs Induction of TNFalpha secretion in human PBMC incubated for 16 hrs	[PMID: 36201304]
PBMC	EC₅₀	26.71 μM Compound: 3',3'-cGAMP	Induction of INF-alpha production in healthy human PBMC incubated for 16 hrs Induction of INF-alpha production in healthy human PBMC incubated for 16 hrs	[PMID: 34019405]
PBMC	EC₅₀	7.65 μM Compound: 3'3'-cGAMP	Induction of IFNalpha secretion in human PBMC incubated for 16 hrs Induction of IFNalpha secretion in human PBMC incubated for 16 hrs	[PMID: 36201304]

In Vitro

cGAMP promotes the antigen-specific proliferation capacity of spleen cells in mice^[2].
cGAMP directly activates murine and human dendritic cells in vitro^[2].
On stimulation with cGAMP, fibroblasts from the patients showed increased transcription of IFNB1 but not of the genes encoding interleukin-1 (IL1), interleukin-6 (IL6), or tumor necrosis factor (TNF)^[3].
cGAMP activates the endoplasmic reticulum (ER)-resident receptor STING, thereby inducing an antiviral state and the secretion of type I IFNs^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

cGAMP (5 μg; nostril mucosal adjuvant) promotes the antigen-specific cytokine production by spleen cells of immunized mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Female C57BL/6 (H-2b) mice 6–8 weeks old^[2]
Dosage:

5 µg
Administration:

Nostril mucosal adjuvant
Result:

Higher titers of ovalbumin (OVA)-specific IgA and total IgG as well as IgG1 and IgG2c in the sera of mice immunized with cGAMP-adjuvanted OVA as compared to sera from OVA-immunized mice.

Chemical Information

CAS. Nr. 849214-04-6
Appearance Solid
Molecular Weight 674.41
Formel C₂₀H₂₄N₁₀O₁₃P₂
Color White to yellow
SMILES

O=P(O[C@H]1[C@@H](O)[C@H](N2C=NC3=C2N=CN=C3N)O[C@@H]1COP4(O)=O)(O)OC[C@@H]5[C@@H](O4)[C@@H](O)[C@H](N6C=NC7=C6N=C(N)NC7=O)O5
Synonyms

Cyclic GMP-AMP; 3',3'-cGAMP
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (27)

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Journal Impact Factor

Signal Transduct Target Ther

Inhibition of long interspersed nuclear element-1 by nucleoside reverse transcriptase inhibitors attenuates vascular calcification. [Abstract]

2025 Oct 1;10(1):321. PMID: 41027876

cGAMP purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Oct 1;10(1):321. [Abstract]

cGAMP accelerated calcium deposition in VSMCs.

cGAMP purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Oct 1;10(1):321. [Abstract]

cGAMP increased the calcium content in VSMCs.
Cancer Cell

Chemotherapy triggers immune evasion by fostering LEPR+ Kupffer cell differentiation in liver metastases. [Abstract]

2026 Mar 9;44(3):658-675.e12. PMID: 41687606
Cell

Divergent tumor immunity determined by bacteria-cancer cell engagement. [Abstract]

2026 Mar 19;189(6):1748-1767.e26. PMID: 41643675
Nat Commun

Oral ENPP1 inhibitor designed using generative AI as next generation STING modulator for solid tumors. [Abstract]

2025 May 23;16(1):4793. PMID: 40410143
Nat Commun

Epstein-Barr virus infection upregulates extracellular OLFM4 to activate YAP signaling during gastric cancer progression. [Abstract]

2024 Dec 4;15(1):10543. PMID: 39627192

cGAMP purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Dec 4;15(1):10543. [Abstract]

cGAMP (2.5 or 10 μM, 24 h) increased OLFM4 protein expression in HGC-27 cells.

cGAMP purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Dec 4;15(1):10543. [Abstract]

cGAMP (10 μM, 6, 12, and 24 h) increased OLFM4 mRNA expression in HGC-27 cells.
Nat Commun

Multi-antigen intranasal vaccine protects against challenge with sarbecoviruses and prevents transmission in hamsters. [Abstract]

2024 Jul 23;15(1):6193. PMID: 39043645
Nat Commun

An intranasal nanoparticle STING agonist protects against respiratory viruses in animal models. [Abstract]

2024 Jul 18;15(1):6053. PMID: 39025863
ACS Nano

Universal STING Pathway-Activating Complexes Counteract Viral Immune Evasion and Boost Antiviral Responses. [Abstract]

2025 Mar 4;19(8):8277-8293. PMID: 39988898
J Nanobiotechnology

Extracellular matrix-degrading STING nanoagonists for mild NIR-II photothermal-augmented chemodynamic-immunotherapy. [Abstract]

2022 Jan 6;20(1):23. PMID: 34991618
Adv Sci (Weinh)

Self-Propelled In Situ Polymerized Nanoparticles Activating the STING Pathway for Enhanced Bladder Cancer Immunotherapy. [Abstract]

2025 Mar 26:e2502750. PMID: 40135781
Cell Death Differ

STING directly interacts with PAR to promote apoptosis upon acute ionizing radiation-mediated DNA damage. [Abstract]

2025 Jun;32(6):1167-1179. PMID: 39939798

cGAMP purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2025 Jun;32(6):1167-1179. [Abstract]

cGAMP (0, 2, and 200 nM; 3 or 6 h) increased STING phosphorylation at 3 h with 200 nM, but not at 6 h in THP-1 cells.
Biomaterials

Regulating T-cell metabolic reprogramming and blocking PD-1 co-promote personalized postoperative autologous nanovaccines. [Abstract]

2023 Jun:297:122104. PMID: 37058898
Chem Eng J

2022: 140190.
J Exp Med

ERAdP facilitates biogenesis of dense core vesicles in Paneth cells to enhance intestinal defense. [Abstract]

2026 Feb 2;223(2):e20251051. PMID: 41474967
Chin Herb Med

Amplifying protection against acute lung injury: Targeting both inflammasome and cGAS-STING pathway by Lonicerae Japonicae Flos-Forsythiae Fructus drug pair. [Abstract]

2024 Apr 30;16(3):422-434. PMID: 39072201
Sci China Life Sci

Targeting STING oligomerization with licochalcone D ameliorates STING-driven inflammatory diseases. [Abstract]

2024 Dec;67(12):2664-2677. PMID: 39190126
Cancer Immunol Res

FAM135B Deficiency Inhibits Cytotoxic T-Cell Activity in Triple-negative Breast Cancer by Blocking the IFI16-dependent STING Pathway. [Abstract]

2025 Nov 11. PMID: 41218197
Cell Rep

Nuclear translocation of cGAS orchestrates VEGF-A-mediated angiogenesis. [Abstract]

2023 Apr 5;42(4):112328. PMID: 37027305
Biochem Pharmacol

STING activation in macrophages by vanillic acid exhibits antineoplastic potential. [Abstract]

2023 Jul:213:115618. PMID: 37211172
Int Immunopharmacol

Minimolide F alleviates inflammatory diseases by specifically targeting STING and blocking IRF3 recruitment. [Abstract]

2026 May 1:176:116468. PMID: 41819671
Int Immunopharmacol

Distinct STING-IRF3 activation determines IL-35+ Breg differentiation in rodent malaria. [Abstract]

2025 Aug 6:164:115310. PMID: 40773895
Antiviral Res

Therapeutic intranasal delivery of NanoSTING provides broad protection against seasonal and highly pathogenic influenza strains. [Abstract]

2026 Mar:247:106342. PMID: 41544777
J Biol Chem

Dynamic control of bacterial antiphage defense through the CdnG-Cap5 cyclic oligonucleotide-based antiphage pathway in Vibrio cholerae. [Abstract]

2025 Dec 6;302(1):111021. PMID: 41360261
Vet Microbiol

Sec6 suppresses BoHV-1-triggered innate immunity through NDP52-mediated autophagic degradation of STING. [Abstract]

2025 Dec 10:312:110836. PMID: 41406560
bioRxiv

It's Not Rewarding for Mitochondria: Dopamine-Induced Mitochondrial Dysfunction Activates cGAS-STING to Drive IL-6 Secretion in Macrophages. [Abstract]

2026 Apr 25:2026.04.23.719926. PMID: 42079251
Patent

US20210017607A1.
bioRxiv

2020 Jul.

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : 180 mg/mL (266.90 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.4828 mL

7.4139 mL

14.8278 mL

5 mM

0.2966 mL

1.4828 mL

2.9656 mL

10 mM

0.1483 mL

0.7414 mL

1.4828 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Wu J, et al. Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA. Science. 2013 Feb 15;339(6121):826-30. [Content Brief]

[2]. Skrnjug I, et al. Cyclic GMP-AMP displays mucosal adjuvant activity in mice. PLoS One. 2014 Oct 8;9(10):e110150. [Content Brief]

[3]. Liu Y, et al. Activated STING in a vascular and pulmonary syndrome. N Engl J Med. 2014 Aug 7;371(6):507-18. [Content Brief]

[4]. Ablasser A, et al. Cell intrinsic immunity spreads to bystander cells via the intercellular transfer of cGAMP. Nature. 2013 Nov 28;503(7477):530-4. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	1.4828 mL	7.4139 mL	14.8278 mL	37.0694 mL
	5 mM	0.2966 mL	1.4828 mL	2.9656 mL	7.4139 mL
	10 mM	0.1483 mL	0.7414 mL	1.4828 mL	3.7069 mL
	15 mM	0.0989 mL	0.4943 mL	0.9885 mL	2.4713 mL
	20 mM	0.0741 mL	0.3707 mL	0.7414 mL	1.8535 mL
	25 mM	0.0593 mL	0.2966 mL	0.5931 mL	1.4828 mL
	30 mM	0.0494 mL	0.2471 mL	0.4943 mL	1.2356 mL
	40 mM	0.0371 mL	0.1853 mL	0.3707 mL	0.9267 mL
	50 mM	0.0297 mL	0.1483 mL	0.2966 mL	0.7414 mL
	60 mM	0.0247 mL	0.1236 mL	0.2471 mL	0.6178 mL
	80 mM	0.0185 mL	0.0927 mL	0.1853 mL	0.4634 mL
	100 mM	0.0148 mL	0.0741 mL	0.1483 mL	0.3707 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

cGAMP

27 Cited Publications

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cGAMP

Macrocyclic Compound Library

MCE Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) cGAMP

Biologische Aktivität

Chemical Information

Publications (27)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

cGAMP

Macrocyclic Compound Library

MCE Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) cGAMP

Biologische Aktivität

Chemical Information

Publications (27)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

cGAMP Related Classifications

HY-12512 Related Classifications