DBCO-PEG4-NHS ester
Based on 4 publication(s) in Google Scholar
DBCO-PEG4-NHS ester is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs. DBCO-PEG4-NHS ester is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
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- Reinheit: 99.00%
- CAS. Nr.: 1427004-19-0
- Formel: C34H39N3O10
- Molecular Weight:649.69
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Speicherung:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) DBCO-PEG4-NHS ester
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Customer Validation & Images
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Biologische Aktivität
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PEGs |
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 1427004-19-0
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Appearance Solid-Liquid Mixture
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Molecular Weight 649.69
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Formel C34H39N3O10
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Color Light yellow to brown
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SMILES
O=C(ON1C(CCC1=O)=O)CCOCCOCCOCCOCCNC(CCC(N2C3=CC=CC=C3C#CC4=CC=CC=C4C2)=O)=O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (4)
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Journal Impact Factor
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Most Recent
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Adv Mater
A Bio-Orthogonal Engineered Chitosan Platform for Enhanced Mesenchymal Stem Cells Delivery and Function in Peripheral Nerve Repair. [Abstract]2026 Mar;38(13):e23237. PMID: 41608909
DBCO-PEG4-NHS ester purchased from MedChemExpress. Usage Cited in: Adv Mater. 2026 Mar;38(13):e23237. [Abstract]
FTIR of DBCO–Chitosan prepared with varying doses of DBCO-PEG4-NHS ester.
DBCO-PEG4-NHS ester purchased from MedChemExpress. Usage Cited in: Adv Mater. 2026 Mar;38(13):e23237. [Abstract]
Representative fluorescence imaging of DBCO-PEG4-NHS ester-modified chitosan membranes incubated with N3–PEG–FITC.
DBCO-PEG4-NHS ester purchased from MedChemExpress. Usage Cited in: Adv Mater. 2026 Mar;38(13):e23237. [Abstract]
Representative stress–strain curves of chitosan membranes with/without DBCO-modified.
DBCO-PEG4-NHS ester purchased from MedChemExpress. Usage Cited in: Adv Mater. 2026 Mar;38(13):e23237. [Abstract]
Scanning electron microscopy (SEM) image of DBCO–Chitosan membranes. Scale bar, 500, and 100 µm (magnification).
DBCO-PEG4-NHS ester purchased from MedChemExpress. Usage Cited in: Adv Mater. 2026 Mar;38(13):e23237. [Abstract]
A live/dead staining assay revealeda decrease in fibroblast viability when the DBCO-PEG4 -NHSester concentration reached 2.5 µm.Cellular toxicity of chitosan materials before and after DBCO modification. a) Live/dead staining of chitosan membranes with or without DBCO modification with Calcein-AM (1:1000) and PI (1:800) staining at 48 and 72 h. Scale bar, 50 μm. b) The cell viability of L929 cells in each group at 48 h (n = 3). Data are expressed as mean ± SD. ****p < 0.001, and ns represent p﹥0.05. Data were analyzed by Two-tailed Student’s t-tests for pairwise group comparisons.
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Adv Sci (Weinh)
Functionalized Biomimetic Nanoparticles Targeting the IL-10/IL-10Rα/Glycolytic Axis in Synovial Macrophages Alleviate Cartilage Degeneration in Osteoarthritis. [Abstract]2025 May 2:e2504768. PMID: 40317692 -
Int J Biol Macromol
Inhibition of ESCRT-independent extracellular vesicles biogenesis suppresses enterovirus 71 replication and pathogenesis in mice. [Abstract]2024 Apr 6;267(Pt 1):131453. PMID: 38588842 -
Lösungsmittel & Löslichkeit
DMSO : 70 mg/mL (107.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.20 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (3.20 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (270 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5392 mL | 7.6960 mL | 15.3920 mL | 38.4799 mL |
| 5 mM | 0.3078 mL | 1.5392 mL | 3.0784 mL | 7.6960 mL | |
| 10 mM | 0.1539 mL | 0.7696 mL | 1.5392 mL | 3.8480 mL | |
| 15 mM | 0.1026 mL | 0.5131 mL | 1.0261 mL | 2.5653 mL | |
| 20 mM | 0.0770 mL | 0.3848 mL | 0.7696 mL | 1.9240 mL | |
| 25 mM | 0.0616 mL | 0.3078 mL | 0.6157 mL | 1.5392 mL | |
| 30 mM | 0.0513 mL | 0.2565 mL | 0.5131 mL | 1.2827 mL | |
| 40 mM | 0.0385 mL | 0.1924 mL | 0.3848 mL | 0.9620 mL | |
| 50 mM | 0.0308 mL | 0.1539 mL | 0.3078 mL | 0.7696 mL | |
| 60 mM | 0.0257 mL | 0.1283 mL | 0.2565 mL | 0.6413 mL | |
| 80 mM | 0.0192 mL | 0.0962 mL | 0.1924 mL | 0.4810 mL | |
| 100 mM | 0.0154 mL | 0.0770 mL | 0.1539 mL | 0.3848 mL |
DBCO-PEG4-NHS ester Related Classifications
HY-140272 Related Classifications
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