Art. -Nr.: HY-103371

DCPIB

11 Cited Publications

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Based on 11 publication(s) in Google Scholar

DCPIB is a selective, reversible and potent inhibitor of volume-regulated anion channels (VRAC). DCPIB voltage-dependently activates potassium channels TREK1 and TRAAK, and inhibits TRESK, TASK1 and TASK3 (IC₅₀s: 0.14, 0.95, 50.72 μM, respectively). DCPIB is also a selective blocker of swelling-induced chloride current (I_Cl,swell), with an IC₅₀ of 4.1 μM. DCPIB is a useful tool for investigating structure-function studies of K2P channels.

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Reinheit: 99.89%
CAS. Nr.: 82749-70-0
Formel: C₂₂H₂₈Cl₂O₄
Molecular Weight:427.36

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

DCPIB Chemical Structure

CAS. Nr.: 82749-70-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (11)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

IC50：0.14 μM (TRESK), 0.95 μM (TASK1), 50.72 μM (TASK3)^[1], 4.1 μM (I_Cl,swell, CPAE cells)^[2]

In Vitro

DCPIB (10 μM) activates TREK1 and enhances TRAAK currents in COS-7 cells^[1].
DCPIB (10 μM) prominently and reversibly suppresses TRESK currents in COS-7 cells, with an IC₅₀ of 0.14 μM^[1].
DCPIB (10 μM) displays selectivity for I_Cl,swell and has no significant inhibitory effects on I_Cl,Ca in CPAE cells^[2].
DCPIB (10 μM, 5 min) has no effect on attenuate subsequent swelling in cardiomyocytes^[2].
DCPIB (10 μM, 3 h) inhibits LPS-induced MAPK activation in BV2 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence^[3]

Cell Line:

BV2 cells
Concentration:

10 μM
Incubation Time:

3 h
Result:

Significantly decreased Ki67 positive staining microglia and pro-inflammatory cytokine (TNF-α, IL-1β) secretion.

Western Blot Analysis^[3]

Cell Line:

BV2 cells
Concentration:

10 μM
Incubation Time:

3 h
Result:

Inhibited migratory potential of transient OGD (Oxygen-glucose deprivation) exposed BV2 cells.

In Vivo

LDN-212854 (intracerebroventricular infusion, 1 mM, 10 μL) suppresses microglial activation and ameliorates neuronal damage in rMCAO rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Reversible middle cerebral artery occlusion (rMCAO) model^[3]
Dosage:

1 mM, 10 μL
Administration:

Administered manually for 20s by intracerebroventricular infusion
Result:

Diminished Pyramidal neurons injury induced by rMCAO in the CA1 region.

Chemical Information

CAS. Nr. 82749-70-0
Appearance Solid
Molecular Weight 427.36
Formel C₂₂H₂₈Cl₂O₄
Color White to off-white
SMILES

O=C(O)CCCOC1=CC2=C(C(C(C3CCCC3)(CCCC)C2)=O)C(Cl)=C1Cl
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (11)

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Journal Impact Factor

Signal Transduct Target Ther

Malate initiates a proton-sensing pathway essential for pH regulation of inflammation. [Abstract]

2024 Dec 30;9(1):367. PMID: 39737965

DCPIB purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Dec 30;9(1):367. [Abstract]

IRF2BP2 protein abundance was visualized by immunoblotting in BMDMs isolated from Hspa5^f/f or Hspa5^f/f; Lyz2-Cre mice. These CHX-treated BMDMs were treated with/without ArA or DCPIB (DB) (100 μM; 9 h). The results showed that DB protected IRF2BP2 from degradation in a BiP-dependent manner.
J Transl Med

ML335 inhibits TWIK2 channel-mediated potassium efflux and attenuates mitochondrial damage in MSU crystal-induced inflammation. [Abstract]

2024 Aug 22;22(1):785. PMID: 39175013
Sci China Life Sci

Osmosensor TMEM63B facilitates insulin secretion in pancreatic β-cells. [Abstract]

2025 Feb 20. PMID: 39985646

DCPIB purchased from MedChemExpress. Usage Cited in: Sci China Life Sci. 2025 Feb 20. [Abstract]

DCPIB (20 μM) blocked SWELL1 activity and decreased the proportion of high-response cells to 65.9%.
Cell Chem Biol

Electrochemical sensor toolkit for simultaneous glutamate detection at edge of cleft and peri-soma. [Abstract]

2025 Jun 19;32(6):885-898.e11. PMID: 40466640
Biochem Pharmacol

A comparative in vitro analysis of pannexin1-targeting agents. [Abstract]

2026 Mar:245:117688. PMID: 41506545
Biochim Biophys Acta Mol Basis Dis

LRRC8A as a central mediator promotes colon cancer metastasis by regulating PIP5K1B/PIP2 pathway. [Abstract]

2024 Feb 11;1870(4):167066. PMID: 38350542
Cell Calcium

The Ca2+ channel TRPV4 is dispensable for Ca2+ influx and cell volume regulation during hypotonic stress response in human keratinocyte cell lines. [Abstract]

2023 May:111:102715. PMID: 36933289
Oncol Rep

Paclitaxel induces pyroptosis by inhibiting the volume‑sensitive chloride channel leucine‑rich repeat‑containing 8a in ovarian cancer cells. [Abstract]

2023 Jun;49(6):115. PMID: 37083067
Biochem J

LRRC8A is responsible for exosome biogenesis and volume regulation in colon cancer cells. [Abstract]

2023 May 17;480(9):701-713. PMID: 37129855
Mol Pain

Upregulation of LRRC8A in the anterior cingulate cortex mediates chronic visceral pain in adult male mice with neonatal maternal deprivation. [Abstract]

2025 Jan-Dec:21:17448069251324645. PMID: 39962353
bioRxiv

Cytoskeletal activation of NHE1 regulates mechanosensitive cell volume adaptation and proliferation. [Abstract]

2024 Apr 18:2023.08.31.555808. PMID: 37693593

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (233.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3399 mL

11.6997 mL

23.3995 mL

5 mM

0.4680 mL

2.3399 mL

4.6799 mL

10 mM

0.2340 mL

1.1700 mL

2.3399 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.08 mg/mL (4.87 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.08 mg/mL.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (4.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (641 KB)
- English - EN (641 KB)
- Français - FR (641 KB)
- Deutsch - DE (641 KB)
- Norwegian - NO (641 KB)
- Español - ES (641 KB)
- Swedish - SV (641 KB)
- Italian - IT (641 KB)
- Korean - KR (641 KB)
- Portuguese - PT (641 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Lv J, et al. DCPIB, an Inhibitor of Volume-Regulated Anion Channels, Distinctly Modulates K2P Channels. ACS Chem Neurosci. 2019 Apr 17. [Content Brief]

[2]. Decher N, et al. DCPIB is a novel selective blocker of I(Cl,swell) and prevents swelling-induced shortening of guinea-pig atrial action potential duration. Br J Pharmacol. 2001 Dec;134(7):1467-79. [Content Brief]

[3]. Qingdong Han, et al. DCPIB, a potent volume-regulated anion channel antagonist, attenuates microglia-mediated inflammatory response and neuronal injury following focal cerebral ischemia. Brain Res. 2014 Jan 13;1542:176-85. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3399 mL	11.6997 mL	23.3995 mL	58.4987 mL
	5 mM	0.4680 mL	2.3399 mL	4.6799 mL	11.6997 mL
	10 mM	0.2340 mL	1.1700 mL	2.3399 mL	5.8499 mL
	15 mM	0.1560 mL	0.7800 mL	1.5600 mL	3.8999 mL
	20 mM	0.1170 mL	0.5850 mL	1.1700 mL	2.9249 mL
	25 mM	0.0936 mL	0.4680 mL	0.9360 mL	2.3399 mL
	30 mM	0.0780 mL	0.3900 mL	0.7800 mL	1.9500 mL
	40 mM	0.0585 mL	0.2925 mL	0.5850 mL	1.4625 mL
	50 mM	0.0468 mL	0.2340 mL	0.4680 mL	1.1700 mL
	60 mM	0.0390 mL	0.1950 mL	0.3900 mL	0.9750 mL
	80 mM	0.0292 mL	0.1462 mL	0.2925 mL	0.7312 mL
	100 mM	0.0234 mL	0.1170 mL	0.2340 mL	0.5850 mL

Keywords

DCPIB

11 Cited Publications

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DCPIB

Membrane Transporter/Ion Channel Compound Library

Mechanoreceptors Compound Library

Targeted Diversity Library

Potassium Channel Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Ion Channel Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) DCPIB

Biologische Aktivität

Chemical Information

Publications (11)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

DCPIB

Membrane Transporter/Ion Channel Compound Library

Mechanoreceptors Compound Library

Targeted Diversity Library

Potassium Channel Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Ion Channel Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) DCPIB

Biologische Aktivität

Chemical Information

Publications (11)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

DCPIB Related Classifications

HY-103371 Related Classifications