Ganglioside GT1b (bovine) ammonium

Ganglioside GT1b (bovine) ammonium (10-40 μg/mL; 5-30 min) induces dose- and time-dependent phosphorylation of Akt and mTOR in orbital fibroblasts from TED cells^[1].
Ganglioside GT1b (bovine) (ammonium) (5-20 nM; 40 h) at 5 nM significantly increases the metaphase II nuclear maturation rate of porcine cumulus-oocyte complexes to 86.6% after 40 h of in vitro maturation, while 10 nM and 20 nM do not produce a significant change^[2].
Ganglioside GT1b (bovine) ammonium (5-20 nM; 40 h) at 5 nM and 20 nM significantly decreases intracellular glutathione levels in matured porcine metaphase II oocytes after 40 h of in vitro maturation^[2].
Ganglioside GT1b (bovine) ammonium (5-20 nM; 40 h) at 10 nM significantly decreases intracellular reactive oxygen species levels in matured porcine metaphase II oocytes after 40 h of in vitro maturation, while 5 nM and 20 nM have no significant effect^[2].
Ganglioside GT1b (bovine) ammonium (5-20 nM; 40 h) at 20 nM significantly decreases bradykinin 2 receptor expression, and at 5 nM, 10 nM, and 20 nM significantly decreases calcium/calmodulin-dependent protein kinase II delta expression in matured porcine cumulus cells after 40 h of in vitro maturation, with no significant effects on proliferating cell nuclear antigen, Bax, Bcl-2, or Caspase-3 expression^[2].
Ganglioside GT1b (bovine) ammonium (5-20 nM; 18-40 h) at 5 nM decreases intracellular calcium levels in porcine oocytes after 18 h of in vitro maturation, while 5 nM and 20 nM increase intracellular calcium levels after 40 h of in vitro maturation, with 20 nM producing a greater increase than 5 nM^[2].
Ganglioside GT1b (bovine) ammonium (10-100 μM; 5 min at 30°C) potently inhibits protein kinase C activity in cytosolic and total particulate fractions from lactating bovine mammary gland, with IC₅₀ values of 20 μM and 28 μM respectively, and this inhibition is reversed by phosphatidylserine but not by OAG or calcium ions^[3].
Ganglioside GT1b (bovine) ammonium (10-100 μM; 5 min at 30°C) selectively modulates PKC-dependent phosphorylation of cytosolic proteins from lactating bovine mammary gland, suppressing phosphorylation of 91 kDa, 89 kDa, 56 kDa, 43 kDa, and 36 kDa proteins (with varying concentration dependence) and enhancing phosphorylation of 72 kDa and 56 kDa proteins at 10 μM, while leaving 72 kDa and 21 kDa protein phosphorylation resistant at 100 μM; suppression of 91 kDa and 89 kDa phosphorylation at 10 μM is reversed by phosphatidylserine^[3].
Ganglioside GT1b (bovine) ammonium (1-100 μg/mL; 1 h pre-incubation, 80 min imaging) inhibits phagocytosis of pHrodo Red E. coli BioParticles by primary mouse mixed glial cells in a dose-dependent manner, with significant suppression observed at concentrations of 1 μg/mL, 10 μg/mL, and 100 μg/mL after 80 minutes of imaging^[4].
Ganglioside GT1b (bovine) ammonium (10 μg/mL; 1 h) significantly reduces SYK phosphorylation in primary mouse mixed glial cells, indicating inhibition of SYK-mediated signaling pathways^[4].
Ganglioside GT1b (bovine) ammonium (1-4 μM; 44 h) enhances meiotic maturation and cumulus cell expansion of porcine cumulus-oocyte complexes by upregulating mRNA levels of HAS2, TNFAIP6, and PTX3^[5].
Ganglioside GT1b (bovine) ammonium (2 μM; 44 h), alone or in combination with 10 ng/mL EGF, enhances meiotic maturation, cumulus cell expansion, and activation of EGFR-mediated ERK1/2 signaling of porcine cumulus-oocyte complexes, with the most pronounced effects observed with combined GT1b/EGF treatment^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.