HNSS peptide
HNSS peptide is a chimeric hybrid peptide composed of the neuroprotective peptide S14G-Humanin and the mitochondria-targeted antioxidant peptide SS31. HNSS peptide is readily cleared in vivo and can be combined with nanocarriers to improve its stability. HNSS can be delivered via the FGFR1-targeting nanocarrier FGL-NP (Cit), ameliorating mitochondrial dysfunction, Aβ deposition, tau hyperphosphorylation, and cholinergic neuronal damage, and alleviating memory deficits in Alzheimer's disease mice. HNSS peptide is applicable for Alzheimer's disease-related research.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Formel: C154H255N45O37S2
- Molecular Weight:3393.08
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
IC50 & Target
[1]|
p-STAT3 |
In Vitro
HNSS peptide is efficiently encapsulated into NP (Cit) nanoparticles via electrostatic interactions, with high encapsulation efficiency and drug loading capacity; after encapsulation, its stability in plasma and brain homogenate is significantly enhanced, and it exhibits pH-responsive drug release characteristics, with accelerated HNSS release under acidic pH 5.0 conditions[1].
HNSS peptide (10 μM) most effectively inhibits H2O2-induced intracellular and mitochondrial ROS production in HT22 cells[1].
HNSS peptide (10 μM; 24 h) most effectively restores mitochondrial function in Aβ25-35 (HY-P0128)-treated HT22 cells by upregulating p-STAT3, MTCO1, and MnSOD, and by improving mitochondrial membrane potential, ATP levels, and cell viability[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HT22 cells
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Concentration:25 μM Aβ25-35 (HY-P0128)
10 μM HNSS preparations -
Incubation Time:24 h
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Result:Strongly upregulated the expression of p-STAT3, MTCO1, and MnSOD.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Molecular Weight 3393.08
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Formel C154H255N45O37S2
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SMILES
O=C([C@H](N)CCCNC(N)=N)N[C@H](C(N[C@H](C(N[C@H](C(NCC(NCC(N[C@H](C(N[C@H](C(N1[C@H](C(N[C@H](C(NCC(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(NCC(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N2[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N)=O)C)=O)CCCNC(N)=N)=O)CCCNC(N)=N)=O)CCCCN)=O)C(C)C)=O)CCC2)=O)CC(C)C)=O)CC(O)=O)=O)[C@H](CC)C)=O)CCC(O)=O)=O)=O)[C@@H](C)O)=O)CC(C)C)=O)CC(C)C)=O)CC(C)C)=O)CC(C)C)=O)CS)=O)CO)=O)CC3=CC=CC=C3)=O)=O)CCCNC(N)=N)=O)CCC1)=O)C)=O)CCSC)=O)=O)=O)CC4=CC=CC=C4)=O)CCCCN)=O)CC5=C(C=C(O)C=C5C)C
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Sequence
DArg-Tyr(2,6-diMe)-Lys-Phe-Gly-Gly-Met-Ala-Pro-Arg-Gly-Phe-Ser-Cys-Leu-Leu-Leu-Leu-Thr-Gly-Glu-Ile-Asp-Leu-Pro-Val-Lys-Arg-Arg-Ala-NH2
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Sequence Shortening
DArg-Tyr(2,6-diMe)-KFGGMAPRGFSCLLLLTGEIDLPVKRRA-NH2
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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Mitochondrial membrane-potential and mitochondrial mass staining
Mitochondrial membrane potential staining measures the electrochemical polarization across the mitochondrial inner membrane in live cells using lipophilic cationic fluorescent probes; early rhodamine-based work showed that selective mitochondrial dye accumulation is lost when the mitochondrial transmembrane potential is dissipated. JC-1 reports mitochondrial polarization by shifting from green monomer fluorescence to red J-aggregate fluorescence as dye concentration increases within energized mitochondria; therefore, the red/green fluorescence ratio is used as a relative readout of mitochondrial membrane potential. TMRE or TMRM staining provides a single-channel relative readout because these cationic rhodamine esters accumulate in polarized mitochondria, and lower fluorescence indicates reduced mitochondrial polarization when acquisition and dye-loading conditions are controlled. Mitochondrial mass staining is commonly performed with MitoTracker Green FM or related MitoTracker dyes as
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Alzheimer’s Disease Modeling
Alzheimer’s Disease (AD) is a neurodegenerative disorder characterized by a progressive decline in cognitive functions and loss of specific types of neurons and synapses. Alzheimer's symptoms can be simulated in mice by injecting drugs (such as Aβ) or genetically modified.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
HNSS peptide Related Classifications
HY-P12338 Related Classifications
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