Schizokinen

Schizokinen (0-200 µM; 48 h) maintains > 95% viability of Huh7 cells up to 200 µM^[1].

Schizokinen is taken up by E. coli, P. aeruginosa, S. aureus, and S. epidermidis, with the highest uptake in S. epidermidis among the tested strains^[5].
Schizokinen has minimal effect on bacterial viability, except in S. epidermidis, where it causes a 1.7-fold reduction^[5].

Schizokinen (24 h) significantly reduces the labile iron pool in iron-loaded Huh7 cells^[1].

Schizokinen (6 min) scavenges ABTS^•+ with a TEAC of 49.8 µM trolox/mM siderophore in the ABTS assay^[1].
Schizokinen (30 min) scavenges DPPH^• with an IC₅₀ of 0.19 mM in the DPPH assay^[1].
Schizokinen (6.25-100 µM; 24 h) reduces ROS levels in iron-loaded Huh7 cells in a dose-dependent manner^[1].

Schizokinen (1 mg/mL; 0.1 M KCl; metal at approximately 42% of maximum Fe content) strongly binds Fe³⁺ and Cu²⁺ across the entire pH range, binds Co²⁺ and Zn²⁺ above pH 6, and does not appreciably complex Mn²⁺ or Mg^2+[4].
Schizokinen shortens the division lag phase of Bacillus megaterium^[4].
Schizokinen (1,000 μg/mL; ferrimycin A 160 μg/mL) reverses the inhibition of Bacillus species by ferrimycin A, but does not reverse the inhibition of Staphylococcus aureus^[4].
Schizokinen (10-50 μg; approximately 10-15 min) is radiolabeled with ⁶⁸Ga to form [⁶⁸Ga]Ga-SKN, with a radiochemical purity of ≥ 95% and a radiochemical yield of 96.91%^[5].
Schizokinen (5 min) is 100% stable after 5 min of incubation in human serum^[5].
Schizokinen (6 mM DTPA; 5-60 min) is not inert to ligand exchange; under challenge with 6 mM Pentetic acid (DTPA) (HY-B1335), it retains approximately 90% integrity at 5 min and approximately 50% at 60 min^[5].
Schizokinen (15 nM; 3-3.5 h) reduces uptake by 9.2-fold for AFS-I-iutA2 and by 7.3-11.5-fold for AFS-I-fhuD/AFS-I-fhuC relative to the wild type in iron-deficient cultures of Anabaena sp. PCC 7120, indicating that IutA2 and FhuCD are involved in uptake^[6].