Siccayne
Siccayne, found in the marine basidiomycete Halocyphina villosa and deuteromycete Helminthosporium siccans, is an antibiotic. Siccayne interferes with nucleotide incorporation into DNA and RNA, precursor transport enzymatic reactions, and essential macromolecule synthesis. Siccayne inhibits Gram-positive bacteria and some fungi. Siccayne can be used for research on infections caused by bacteria and fungi.
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- CAS. Nr.: 22944-03-2
- Formel: C11H10O2
- Molecular Weight:174.20
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
Beschreibung
In Vitro
Siccayne inhibits the growth of select Gram-positive bacteria (with MICs from 10 to 50 μg/mL) and a small subset of fungi (at 50 μg/mL), while most tested bacteria and fungi are resistant at concentrations >50 μg/mL[1].
Siccayne (2-10 μg/2×106 cells) potently inhibits DNA synthesis (to ~10% of control) at 10 μg/2×106 cells in both normal and Rous-sarcoma-virus-transformed chicken embryo fibroblasts, with weaker inhibition of RNA and protein synthesis[1].
Siccayne (100 μg/mL) causes slight inhibition of DNA synthesis in toluene-treated Bacillus brevis cells, reducing dTTP incorporation to 62% of control at 100 μg/mL[1].
Siccayne (100 μg/mL) does not induce hemolysis of bovine erythrocytes at concentrations up to 100 μg/mL[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS. Nr. 22944-03-2
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Molecular Weight 174.20
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Formel C11H10O2
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SMILES
OC1=CC=C(C(C#CC(C)=C)=C1)O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.
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Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Siccayne Related Classifications
HY-182477 Related Classifications
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