Tyrosinase, Mushroom

Product Information
Optimal pH: 6.0-7.0
Theoretical molecular weight: 128 kDa (tetramer)

Tyrosinase activity assay
Enzyme solution preparation
Dissolve in ddH₂O at a concentration of 1 mg/mL. Further dilute to a concentration of 200-400 U/mL.
Experimental method
Adjust the spectrophotometer to 280 nm and 25°C. Add the sample to each colorimetric tube in the following order:
1mL 0.5 M Phosphate buffer, pH 6.5
1 mL 0.001 M L-Tyrosine
0.9 mL ddH₂O
Oxygen is introduced into the colorimetric tube through a capillary for 4-5 min to oxidize the reaction mixture. Place the colorimetric tube on the spectrophotometer and record the A280 at 4-5 min as the absorbance of the blank group. Add 0.1 mL of appropriately diluted enzyme and record the A280 at 10-12 min.
Calculation
1U = the amount of enzyme that causes the absorbance at 280 nm to increase by 0.001 per minute.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.