DMN-Tre
Based on 1 Customer Validation
DMN-Tre is a conjugate of a solvatochromic fluorescent dye and trehalose. DMN-Tre takes advantage of the substrate promiscuity of the endogenous antigen 85 protein complex in mycobacteria to be metabolically integrated into the hydrophobic mycobacterial membrane. Once entering this hydrophobic environment, the linked DMN dye fluorescence is "turned on", enabling specific labeling. DMN-Tre can be used to reflect bacterial metabolic activity and support physiological studies of Mycobacterium tuberculosis.
For research use only. We do not sell to patients.
- Purity : 98.87%
- CAS No.: 2177287-88-4
- Formula: C26H32N2O12
- Molecular Weight:564.54
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Guide (The following are our recommended solutions. These are only Guide and should be modified according to your specific needs).
1.Preparation: Prepare or obtain DMN-Tre stock solution.
2. Incubation: Incubate samples with a final concentration of 100
M (for M. smegmatis) or up to 1 mM (for M. tuberculosis (Mtb)) of DMN-Tre.
3. Time: For M. smegmatis, 30 min is typical. For Mtb in sputum or cultures, labeling may take up to 16 hours or overnight.
4. Detection/Fixation: The cells can be imaged directly. For clinical sputum samples, this is followed by fixation (e.g., 4% formaldehyde or 2.5% glutaraldehyde) before analysis with a fluorescent microscope or flow cytometer.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 2177287-88-4
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Appearance Solid
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Molecular Weight 564.54
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Formula C26H32N2O12
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Color Yellow to orange
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SMILES
O=C1C2=CC=C(N(C)C)C3=C2C(C(N1C[C@H]4O[C@@H]([C@@H]([C@H]([C@@H]4O)O)O)O[C@H]5O[C@@H]([C@H]([C@@H]([C@H]5O)O)O)CO)=O)=CC=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
In Vitro:
H2O : 20 mg/mL (35.43 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Immunoprecipitation
Immunoprecipitation (IP) is an experimental method that uses the principle of antibody specific binding to purify and enrich target proteins.
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MTT Cell Proliferation Assay
The MTT assay is a colorimetric endpoint assay for estimating viable cell number, cell growth, cytotoxicity, or cell activation in cultured mammalian cells. Living cells reduce the yellow tetrazolium salt MTT into purple/blue formazan, while dead cells do not generate the same signal; the resulting color can be quantified with a multiwell spectrophotometer. MTT reduction is commonly interpreted as a readout of metabolic activity that often correlates with viable cell number, but it should not be treated as a direct cell-counting method unless the assay is optimized for the cell type and experimental condition. Studies show that MTT reduction can involve mitochondrial and non-mitochondrial reducing systems, and formazan may accumulate in intracellular lipid droplets rather than simply marking mitochondria.
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Co-Immunoprecipitation
Co-immunoprecipitation technology can verify protein interaction based on the specific immune reaction between antibodies and antigens.
Purity & Documentation
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Data Sheet (292 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.7714 mL | 8.8568 mL | 17.7135 mL | 44.2838 mL |
| 5 mM | 0.3543 mL | 1.7714 mL | 3.5427 mL | 8.8568 mL | |
| 10 mM | 0.1771 mL | 0.8857 mL | 1.7714 mL | 4.4284 mL | |
| 15 mM | 0.1181 mL | 0.5905 mL | 1.1809 mL | 2.9523 mL | |
| 20 mM | 0.0886 mL | 0.4428 mL | 0.8857 mL | 2.2142 mL | |
| 25 mM | 0.0709 mL | 0.3543 mL | 0.7085 mL | 1.7714 mL | |
| 30 mM | 0.0590 mL | 0.2952 mL | 0.5905 mL | 1.4761 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
DMN-Tre Related Classifications
HY-D2919 Related Classifications
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