L-012
Based on 2 publication(s) in Google Scholar
L-012 is a luminal-based chemiluminescent probe. L-012 sodium salt can detect NADPH oxidase (Nox)-derived superoxide and nitrogen species (reactive oxygen species (ROS) and reactive nitrogen species (RNS)).
For research use only. We do not sell to patients.
- Purity: 99.63%
- CAS No.: 143323-55-1
- Formula: C13H9ClN4O2
- Molecular Weight:288.69
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) L-012
More
Customer Validation & Images
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Bio/Physico-chemical Assay
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Histological Imaging/Staining
Biological Activity
1. Solution preparation[1]
1.1 Preparation of stock solution
Solvent: DMSO or ultrapure water
Concentration: 10 mM is recommended.
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 1-10 μM with PBS or serum-free cell culture medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.
2 Assay for ROS Generated by Cell-Free Systems.
2.1 In the presence of 1 mM hypoxanthine in KRP (Krebs-Ringer phosphate buffer), superoxide is generated by xanthine oxidase (6.4 mU/mlL) at 37°C.
2.2 Concentrations of L-012, luminol and MCLA used for the assay are 4 μM, 100 μM, and 4 μM, respectively.
2.3 The hydroxyl radical is generated in KRP by incubating 100 μM DTPA, 1 mM H2O2, and 50 μM FeSO4 at 37°C.
2.4 Detection using in Luminescence Reader.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Luciferase activity was monitored in vivo by injecting the substrate luciferin.
L-012 (25 mg/kg in 100 μL) is administered intravenously (iv), intraperitoneally (ip), or subcutaneously (sc).
Detection using in vivo imaging.
Image exposure times are between 5 s and 2 min, depending on the signal strength.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 143323-55-1
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Appearance Solid
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Molecular Weight 288.69
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Formula C13H9ClN4O2
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Color Light yellow to yellow
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SMILES
O=C(C1=C2C(Cl)=NC(C3=CC=CC=C3)=C1N)NNC2=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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Mater Today Bio
Multifunctional human serum albumin-crosslinked and self-assembling nanoparticles for therapy of periodontitis by anti-oxidation, anti-inflammation and osteogenesis. [Abstract]2024 Jul 22:28:101163. PMID: 39183771
L-012 purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2024 Jul 22:28:101163. [Abstract]
Live imaging of small animals and quantitative analysis of ROS stained by L012 in periodontal tissue with different treatments (7d).
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Cell Calcium
A NOX2-independent mechanism of Hv1 channel activation promotes inflammatory cytokine release from BV-2 microglia via intracellular Ca2+ mobilisation. [Abstract]2026 Mar:134:103122. PMID: 41581267
L-012 purchased from MedChemExpress. Usage Cited in: Cell Calcium. 2026 Mar:134:103122. [Abstract]
Activity of NOX2 in cells treated with 100ng/ml PMA, PMA+ 30 μM GSK2795039and 10 μM S-023–0515 was measured by L-012 (10 μM, 10 min at 37 °C) luminescence kinetics over 90 min.
Solvent & Solubility
DMSO : 100 mg/mL (346.39 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Imada I, et al. Analysis of reactive oxygen species generated by neutrophils using a chemiluminescence probe L-012[J]. Analytical biochemistry, 1999, 271(1): 53-58. [Content Brief]
[2]. Kielland A, et al. In vivo imaging of reactive oxygen and nitrogen species in inflammation using the luminescent probe L-012. Free Radic Biol Med. 2009;47(6):760-766. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4639 mL | 17.3196 mL | 34.6392 mL | 86.5981 mL |
| 5 mM | 0.6928 mL | 3.4639 mL | 6.9278 mL | 17.3196 mL | |
| 10 mM | 0.3464 mL | 1.7320 mL | 3.4639 mL | 8.6598 mL | |
| 15 mM | 0.2309 mL | 1.1546 mL | 2.3093 mL | 5.7732 mL | |
| 20 mM | 0.1732 mL | 0.8660 mL | 1.7320 mL | 4.3299 mL | |
| 25 mM | 0.1386 mL | 0.6928 mL | 1.3856 mL | 3.4639 mL | |
| 30 mM | 0.1155 mL | 0.5773 mL | 1.1546 mL | 2.8866 mL | |
| 40 mM | 0.0866 mL | 0.4330 mL | 0.8660 mL | 2.1650 mL | |
| 50 mM | 0.0693 mL | 0.3464 mL | 0.6928 mL | 1.7320 mL | |
| 60 mM | 0.0577 mL | 0.2887 mL | 0.5773 mL | 1.4433 mL | |
| 80 mM | 0.0433 mL | 0.2165 mL | 0.4330 mL | 1.0825 mL | |
| 100 mM | 0.0346 mL | 0.1732 mL | 0.3464 mL | 0.8660 mL |
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