Rhodamine 6G

Guide (The following is our recommended experimental protocol. This protocol serves merely as a reference guide; specific procedures should be adjusted according to your actual requirements.)
1. Preparation of Rhodamine 6G Working Solution
1.1 Preparation of Stock Solution
Dilute 1 mg of Rhodamine 6G with 525 μL of anhydrous DMSO to prepare a 5 mM stock solution.
1.2 Preparation of Working Solution
Dilute the stock solution with pre-warmed serum-free cell culture medium or PBS to prepare a Rhodamine 6G working solution with a concentration of 1–20 μM.
Note: Please adjust the concentration of the Rhodamine 6G working solution according to your specific needs, and prepare it immediately before use.
2. Cell Staining (Suspension Cells)
2.1 Centrifuge to harvest the cells, then wash twice with PBS (5 minutes per wash). Adjust the cell density to 1 × 10⁶ cells/mL.
2.2 Add 1 mL of the Rhodamine 6G working solution and incubate at room temperature for 30–60 minutes.
2.3 Centrifuge at 400 × g for 3–4 minutes, and discard the supernatant.
2.4 Wash the cells twice with PBS (5 minutes per wash).
2.5 Resuspend the cells in 1 mL of serum-free medium or PBS, then observe using a fluorescence microscope or flow cytometer.
3. Cell Staining (Adherent Cells)
3.1 Culture the adherent cells on sterile coverslips.
3.2 Remove the coverslips from the culture medium and aspirate any excess medium.
3.3 Add 100 μL of the dye working solution, gently agitate to ensure the solution completely covers the cells, and incubate for 5–30 minutes.
3.4 Aspirate the working dye solution, wash with culture medium 2–3 times (5 minutes each), and observe using a fluorescence microscope or flow cytometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.